Work on cleanliness, a SAB works just as good if you use them right. I have both a SAB and FFU and I'll be honest I use my SAB way more because it provides way more space. FFU's are usually just for commercial growers or growers on a larger scale because it's alot quicker. Alls ik is be super clean and you won't have a problem.
I make steril Rye in Bags....frozen! -30Grad celsius.All Bakterian life died.The Question is...the Myzelium love the frozen Rye...havr Scar,s and colonisation very quickly!!!
You can try breaking it up and shaking it but when it stalls out I haven't seen them come back to life. I noticed mine stalling when the grain didn't absorb enough water before sterilization.
Remember to work a bit higher as your plates are directly on the desk bu the flow hood blows turbulent air in this area. Just use a wire rack, that should do the trick :)
Remember kids, bleach + rubbing alcohol = chloroform. If your SAB is all bleachy inside, don't be spraying alcohol in there! Also, bleach & water isn't very effective at sanitizing plastics if you're just going to spray and wipe it around. It beads up on the plastic and therefore doesn't provide the necessary contact time. Instead, use a bleach based disinfectant that has the surfactants necessary to wet-out the surfaces you're trying to disinfect. Something like Clorox Bleach Foamer, or Clorox Bathroom with Bleach. And don't be cheap and simply add dish soap to a bleach solution- the two will react and release poisonous chloromine gases.
This is a good point. I use star san which is specifically meant for surface sanitizing using phosphoric acid. When you spray, it doesn't just bead up but clings and spreads on the surface. It is even food safe and good to use in kitchen
Well that explains why I feel like I have pneumonia my lungs are shot from cleaning with both bleach and alcohol I didn’t know it created chloroform, thanks for the info!
Great video, quick note (a bit nit picky but you may find it interesting) you can usually tell the difference between spore prints and transfers because there will be a raised surface(where you dropped your agar)- and likely more Rhizomorphic growth (only the case with transfers, let’s say you use 10 agar for a spore syringe you will have a hell of a time distinguishing them unless only one shows Rhizomorphic growth). Rhizomorphic growth is seen as ideal because it will inoculate spawn faster, however some claim it has the chance to weed out great genetics because the colonies DNA pool shrinks with each transfer. TLDR- Probably not a super useful comment, but if your a mushcult nerd like myself you might find it interesting.
Likely you were sold some very old agar plates, the cohesive material can become “glued” if left on waaaay too long, if this is the case the agar is likely dry and unsuitable for cultures anyhow. Did you ever fix this problem? Or did you just purchase new agar? If you have not seen Philly Golden Teachers Ez as “123” agar recipe give that a view and a shot, agar is not as difficult as people make it out to be and learning it early on is the key to success with this hobby. Mush luck! Edit- If you do PGT 123 Tek DO NOT use Oven Tek for the agar pour, you might get lucky if your in a new house but you will likely have a much higher contamination rate than with SAB.
I've been cultuvating for a little over a year now and have made several hundred plates. I have never used a laminar flow hood and have only ever had one plate contaminated. Proof if you take your time and keep everything clean, you don't really need one.
Nice! How many CFM is your pc fan? Is it a 120mm pc fan? Second question, did it works well (i mean, no contaminations)? And last question do you think it is better to use a larger box than your or not ( i mean the space between the filter and the fan) ? Thank you!
This is the fan, amzn.to/3B87mJI its adjustable speed. I put a flame to the front and power it up and adjust the fan speed until the flame is bending but stays lit across the entire surface. Personally, I prefer more space. It works great as long as you don't leave the clean air. With the size being as small as it is, it's easy to let your hand get out of the clean air. I never had any contam show up on the plates I tested. I left the plates uncovered for 2 minutes sitting directly on the table (not up in the air like is recommended for the cleanest air) and I kept them for over 4 months.
Thank you for your risponse, i will probably build one like this, very cool design! I just don't know if i have to let more space between the fan and the Hepa filter or not. I see that lots of flowdood have like 30cm of space beetween the back and the front (filter). I dont know it will maybe creat à more laminar homogenous flow than puting the fan just directly the filter? And also if it's better to put the fan on the top or on the side or on the back? I just don't know... maybe you know? But very Nice design! Looks great!😊 and very cool to hear that it's effective!
this is not a laminar flow hood. This does not provide laminar flow, nor does it have a hood apparatus. Cool little design though, if it works well. Dont need actual laminar airflow, nor the hood to get some good work done. Could possibly help out a lot of people!
Yeah, it works as intended but you're right about the laminar part. I'm going to make another video and make that a little more clear. Appreciate the feedback.
@@WhiteBeardHashtag1205 shoot me an email, I'm down to connect. I've got a few questions since you've got a cultivating channel as well. dj@mushlovebewellfarms.com
I keep seeing people everywhere saying this about lots of flowhoods not being laminar flow,but so far I haven't seen what actually makes it laminar flow that these hoods don't meet?
@@mushlovebewellfarms hell yeah bro, Ill shoot you an email. Even though you tagged me, I didnt see this message till now when I saw Relic Boxing responded to me. That happens a lot for some reason. Weird, right?
I bought "rye berries" and took them to the feed store, the guy showed by whole wheat oats and they looked the same to me. Perhaps I was given bad info?
@MushLoveBeWellFarms Yeah there are a few grains out there, My guess is they got it wrong as a one-off. There are Whole Oats, Millet, Milo, Popcorn Kernels(Food grade), Rye berries, WBS (Wild Bird Seed), and Brown Rice. There's more but those are the main ones used as grain spawn
