In this video I show you how to make a Still Air Box out of a 65 Quart plastic tub, and I go over how to use the new SAB. You can find more of my content as mushlovebewellfarms.com
Great video, quick note (a bit nit picky but you may find it interesting) you can usually tell the difference between spore prints and transfers because there will be a raised surface(where you dropped your agar)- and likely more Rhizomorphic growth (only the case with transfers, let’s say you use 10 agar for a spore syringe you will have a hell of a time distinguishing them unless only one shows Rhizomorphic growth). Rhizomorphic growth is seen as ideal because it will inoculate spawn faster, however some claim it has the chance to weed out great genetics because the colonies DNA pool shrinks with each transfer. TLDR- Probably not a super useful comment, but if your a mushcult nerd like myself you might find it interesting.
Likely you were sold some very old agar plates, the cohesive material can become “glued” if left on waaaay too long, if this is the case the agar is likely dry and unsuitable for cultures anyhow. Did you ever fix this problem? Or did you just purchase new agar? If you have not seen Philly Golden Teachers Ez as “123” agar recipe give that a view and a shot, agar is not as difficult as people make it out to be and learning it early on is the key to success with this hobby. Mush luck! Edit- If you do PGT 123 Tek DO NOT use Oven Tek for the agar pour, you might get lucky if your in a new house but you will likely have a much higher contamination rate than with SAB.
Remember kids, bleach + rubbing alcohol = chloroform. If your SAB is all bleachy inside, don't be spraying alcohol in there! Also, bleach & water isn't very effective at sanitizing plastics if you're just going to spray and wipe it around. It beads up on the plastic and therefore doesn't provide the necessary contact time. Instead, use a bleach based disinfectant that has the surfactants necessary to wet-out the surfaces you're trying to disinfect. Something like Clorox Bleach Foamer, or Clorox Bathroom with Bleach. And don't be cheap and simply add dish soap to a bleach solution- the two will react and release poisonous chloromine gases.
This is a good point. I use star san which is specifically meant for surface sanitizing using phosphoric acid. When you spray, it doesn't just bead up but clings and spreads on the surface. It is even food safe and good to use in kitchen
Well that explains why I feel like I have pneumonia my lungs are shot from cleaning with both bleach and alcohol I didn’t know it created chloroform, thanks for the info!
