Some times when sequencing on an ion torrent machine, there is a barcode that takes up a lot of data space, causing the sample data to be insufficient for analysis. But still on this Chip sequencing, re-process the chip: wash the chip, then denature again with primer sequencing and wash the chip again and add the sequencing enzyme and then run the sequencing again, then the No barcode is almost reduced. . Is there any way to reduce the No barcode at the beginning of sequencing after loading the denatured sample onto the sequencing chip?
Omggggggg i can't thank you enough!!!! I have a major test day after tomorrow and was so confused about the adaptors and emPCR. THIS IS THE BEST VIDEO ON PYROSEQUENCING ! You deserve more views girllll ❤
After 4 youtube videos that really lacked explaining a lot of the major details, I found this video. Great explanation and annotation. Thank you so much for the work, effort, and knowledge you present to us!
this video should have so many more clicks! like I watched every single video on yt about the chain termination method and this was the only one that explained it clearly but enough detailed, great work on this
Fantastic video, super clear, easy to follow, great visuals. You could barely even call me a biology student and I still had no problem understanding a technology that would normally be incomprehensible to me 😂
Im Working in laboratory Where we Doing Only Coivd RNA amplification using ( BIORAD ) to identify whether it is present or not . But Im fully interested To learn molecular techniques. now I am job seeker like Project assistant/Project associate/Junior Research Fellow etc ... Is there any chance get jobs like these in Canada I'm ready but I don't know how to execute if u don't mind help me .
Duration 0.13 l have template DNA tha I'm curious to know the sequence Duration 0.30 Primer Annealing My Doubt 1. If want to know the sequence mean u don't know the sequence so u need to know 2. At 0.30 show the primer which attached to the SSDNA. You don't know the seq of your template 🤔🤔🤔then how your primer recognizes it
you can clone the DNA into a vector and design primers (because we know the sequence of the vector) at the beginning and ending of our insert (our unknown DNA). now the polymerase will start at the beginning of our insert and amplify it.
Thank you so much that was a lot easier to understand!! If you don't mind can u send me the presentation you worked with I'd like to use the figures but I couldn't find them anywhere on the internet...
Thanks it was great! But I don't understand how using a capillary gel electrophoresis would help not making separate reaction mixtures. I would love an explanation on that. Thanks again!
this is the first time that I write a comment on yt, but wow! what an amazing video! super explanatory from creating libraries to pyrosequencing! thanks!!
Question about the emulsion-PCR: in the illustration, there is only ONE single DNA-strand which is not linked to the bead. That would mean: 60 cycles -> only 60 copies. Do they additionally use primers for the red adapter to get more copies?
Thank you for your videos! They are really needed! Great choice of subjects, I really appreciate your effort. You helped me understand or revise subjects that I really find important for my study. Thank you!
The PPi that is released from apyrase when no light is detected, does that react with sulfurylase which converts it to ATP? and do we still see light? (Apologies for bad English)
I am not 100% sure but I believe Apyrase releases 2Pi molecules instead of a single PPi molecule when degrading the dNTPs in a well. These Pi molecules are not sufficient for the sulphyrase reaction that makes ATP. Subsequently, the reaction with luciferase does not occur since ATP has not been made.
Here's a link showing all the reactions that take place during pyrosequencing: www.researchgate.net/figure/Enzymatic-reactions-involved-in-pyrosequencing_fig3_268048875
I am impressed with the clarity of the presentation. The drawings mesh perfectly with the carefully chosen wording really get at the "Why" of how this works.
Thank you! Working on transitioning into animating the videos so hopefully they do a better job in visualizing than a static image. I just uploaded one!
