Channel prepared for certain presentations related to certain topics in the interest of the students of the global requirements and certain nonacademic videos (censored and rightful) for all age audiences. We are committed to giving pure content prepared by us as videos/presentations. The topics can be of any stream/field.
Nice detailed illustration of method .I have some questions .can we use fresh cut vegetable sample for extraction or only dried sample is used ? should we dry the sample in oven ?some dilute FCR to 2 times only.20 % Na2CO3 is mentioned in some articles. which is correct concentration ?In one book Instead of gallic acid pyrocatechol is suggested for standard preparation .is it right ?total concentration of gallic acid is calculated in which volume ? 1 ml or 11 ml ?from y=mx + b x is obtained by x=(y-b)/m formula but how x=(y+b)/m derives from y=mx+b ?
Thank you so much for the useful details. Is the Scavenging Ability (%) the same as RSA(%)? For example, the Scavenging ability of Trolox after calculation using [(A0-A1)/A0] formula is 15.16%, so can I write it as "RSA(%)=15.16%"?
Hello Sir, your videos are truly helpful with great explanations. It's like having a guide through all the analyses methods. Thank you so much Sir ❤. I hope you'll upload again soon. 🙏
thank you so much for sharing this! I want to ask, we are doing ABTS test in our lab, however. it was quite confusing for us, cuz when we dissolved our sample with ethanol, we got a negative value of %RSA. Is there anything that we could do to improve our results? some of our samples are partially dissolved in water.
Dear sir, I'm confused how taking 1,2,3,5ml from stock solution and adding into 50ml v.flask and adding distilled water upto ml will give conc. of 50,100,150,500 mcg???
Yes, correct... When you observe the slide, 'reagents required' under this section, ' Preparation of standard gallic acid solution' clearly mentions preparing the concentration of the solution at 50 mcg/ml. (Don't get confused between signs - mcg/ml and μg/ml both are 'microgram per ml'). So, now standard 1ml of gallic acid contains 50 mcg/ml (μg/ml) of gallic acid. Now in the slide - 'procedure' the 1st point mentioned is - different concentrated solutions as 1, 2, 3, 5, 7 & 10 ml of 6 test tubes each containing Gallic acid of 50μg, 100μg, 150μg, 250μg, 350μg and 500μg respectively in 50 ml std volumetric flask - VF. Here, 1, 2, 3, 5, 7 & 10 ml (50μg, 100μg, 150μg, 250μg, 350μg and 500μg) are the respective concentrations taken from the standard gallic acid are the multiples of 50mcg/ml (μg/ml). I hope I have answered your query.
You have considered different dilutions od same sample and therefore you took average but whaht if i have 16 different sample types of same dilution? Can you please explain the calculation for that case.
We have used two different control preparations to mark the difference that also comes under the control. Hence, we have noted the same values. And you don't try the same. Note the control absorbance value for ASA acid and sample should be the same. Thank you for the feedback.
We have used two different control preparations to mark the difference that also comes under the control. Hence, we have noted the same values. And you don't try the same. Note the control absorbance value for ASA acid and sample should be the same. Thank you for the feedback.
when i added 5 ml DW at last. The color changed from dark green to brick red. and the absorbance was totally different as follows. Glucose -working standard(10 ml stock sol in 100 ml DW)-Absorbance at 620nm 1.Blank-0.971 2.0.2ml-2.147 3.0.4ml-2.474 4.0.6ml-2.660 5.0.8ml-2.663 6.1ml-2.800 7.Sample 1-1.354 8.Sample 2-1.233 9.Sample 3-1.459 10.Sample-4-2.055 11.Sample-5-1.685
I think u didn't get the results.. because the recorded absorbance readings are greater than 2.. As you have missed the steps somewhere.. please repeat the experiment..
In this experiment, distilled water is the diluent.. you can decrease the intensity of color; if it is highly dark green, then try by adding extra distilled water of 3 ml in each test tube.
Can you explain about genetic engineering to make enzymes that are tolerant of oxidation, such as by adding antioxidant groups or domains to enzymes? I would be grateful if you can
Thank you, and will develop slides for asked topic. Kindly share some links you refering for it.. So we can add both your references and our references also on slides..
Sir , What is the use of standard solution and working solution in this dilution of standard curve if we directly dissolved 0.18 mg of glucose in 100ml.
if we are using replicates of each dilution of samples then how to calculate Net AUC? Can we take average of AUC of each dilution? Thank you so much for your video
