【4K】-- Column Chromatography (Purification)🌟 

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explanation is excellent and Dr is helpful, this video is the best on youtube.

thank you so much Kevin!

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Thanks a lot 😊

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wow what a racist!

+SixTough John?

+FunMan Fung hi no we dont know each other but grat videos man

+FunMan Fung Thanks, lad, from the Czech rep. ;)

Most Welcome, mate.

+SixTough LOL you didn't get it, but hey thanks!

Thank you very much!

Try to test various solvents to dissolve, such as acetone, acetonitrile, ethyl acetate, hexane , etc !

Yes we can

Why do you want to store?

@@chemfunman Thank you for the reply.. I am not sure, I need to purify enough amount, like around 20-30 litres of bacterial culture, so I was wondering if we can store the resin and reuse it. So is it possible?

The product is probably a solid - so after you purify it by flash column, and you evaporate off all of the solvent (the hard vacuum step), you'll get a solid.

plz doctor i need your answer,tq

No need

I will use the same method to isolate CBD from a THC/CBD oil. Hexane is used as a solvent in this case. You can use isopropyl alcohol or ethanol for your experiment with CBD, that's common, and it works the same as hexane as a suitable solvent. Mention that isopropyl alcohol is as toxic as hexane. Please make sure to dissolve all solvent by heating it above 80°C. Good Luck!

​@@jonasschweiger8938 No you can not simply exchange hexane with isopropyl alcohol, unless you move to reverse phase. Alcohols are very strong eluents, much stronger than esters. I would start with an ether first, but keep in mind, that it is somewhat more polar and an hydrogen bond acceptor, but not a donor, so the selectivity might be off. I think diethyl ether might be convenient. You should never underestimate the toxicity of n-hexane. It is neurotoxic and for some complicated reason much more so than other light alkanes. Modern medical grade petroleum spirit is often explicitly hexane free because of this. Good luck with your projects. And always keep in mind, that natural product separations are often on a different level than simple synthetic workup. This is the real deal for which chromatography was invented and not the harmless stuff encountered in undergraduate organic courses :3 Also one has to keep in mind, that solvents dissolve in your terpenoids (cbd and thc) very well, so they will not be removed by simply heating to their normal boiling point. To get entirely rid of them will need high vaccum and long time periods. Traces are really, really hard to remove even at high temperatures that would harm your products. I am not joking, this is a real problem and most laboratory workers ignore it, because in an organic laboratory, solvent traces do not matter that much. We learned it on an industry course about separation problems some months ago.

Two spoonfuls

It's my pleasure

Sand will protect the silica layer from being disturbed, so that the solute mixture will move as a uniform band (doing its best to). If there are no sand, during the transfer of eluent into the column (while topping up), the silica may experience a 'dent' and not be levelled.

not to disturb the silica layers. sand is denser, it will provide a firmer support.

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Fern Sutiyawan can't use water!! 0.3cm thick sand

Sorry 😞 I don’t understand

Probably carotenoids

try?

good solubility

Yes, soon

@@chemfunman thank you Dear doctor

Buy fine sand from the lab?

yup

I will have to visit the lab and ask. Can u see from the video? at the beginning

Please give information about the air pump that you have used

plz doctor i need your answer,tq

the solid would not dissolve sometimes, and it is OK! When I performed purification using column chromatography, i had many solid particles (insoluble) and did a successful separation any way!!

@@chemfunman thank you doctor for reply. sorry you mean just add the solid that insoluble direct above sand after that you got nice separation

@@chemfunman also i habe this question .if i did some experiments in the lab and my product was (liquid or oily) here how can run column,? i mean add it direct over sand or also must add solvent to product, (this solvent is the same mobile phase or any solvent just until dissolve?)

non polar solvents

to block the silica from fleaking down.

@@chemfunman Is cotton not enough

@@chemicalsociety9094 Nein

positive interaction (bond formation) is an exothermic process. giving out heat

@@chemfunman hmmm... I have some Googling to do. Thanks for the quick reply/answer! Earned my subscription (WITH notifications!) Haha

@@chemfunman thanks for the reply

Can you specific what chemicals you need advice on their quantity?

Fun Man FUNG the n-hexane, silica gel, sand and cotton wool.

@@erika_890 I thought i mentioned in the video. cotton wool, a small glob. silica gel, depends on your diameter of the column, n-hexane, prepare 250 mL

Still the same technique, use a locating agent to identify

NITROGEN / air

@@chemfunman May I ask what brand/model? Thanks again 😊

@@nocturnalrph1678 hah! That I am not sure because there's no brand I noticed:(

@@chemfunman Oh. It's okay. Thanks :)

brand?

Fun Man Fung I mean a name that company uses for its products (trademark) , I want to buy a small pump like yours

@@henrythierry1778 now we under lockdown, no access to lab. we will find after we can go back to work

Fun Man Fung thanks a lot

thanks for the note, Yes! Absolutely right.

normal air would do.

@@chemfunman thank you sir 🙏

Thanks for your support!

@@chemfunman sir, can I get your mail ID? . I have some quarries as I am doing column in my PhD.

@@shravankumbhare5785 let me think about it as I am very occupied with projects

Better do it! if not too many actions at the same time will be distracting. thank you! do share these videos and subscribe.

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Help us translate please?