🍄 Shroom Academy how to make a liquid mushroom culture or liquid mycelium 

Where did u get the spores in india from?

Mycelium can survive underground with very little oxygen. The amount of oxygen in the vial will be sufficient for the tiny amount of mycelium growing there, especially if it's constantly cooled

@@shroomacademy4367 thanks

Too much nutrition might be bad. Too little nutrition is not always good. The amount of nutrients should be optimal. Every species and even strain has a specific type of nutrients and their optimal concentration range.

You should not allow any caramelization to occur. Such substrate is know to be unviable at least for most fungi.

Any updates

Your best chance is that you will catch some spores. So go for the spore gills :)

No it's not possible. Dried tissue is dead tissue. If the sample tissue happens to contain spores - they will most likely germinate however.

@@shroomacademy4367 Are you sure about that?

We are pretty positive about that. Dry fungi matter is dead. But there is a chance that one could get some spores on dried fruiting bodies so it can still germinate. However the sterility is very much compromised in this case

Liquid culture solution (already viable mycelium) or liquid spore solution (spores will germinate and produce mycelium, spores are generally slower to colonize the liquid culture Medium)

It should be kept in dark place at the temperature suitable for spawning/incubation that corresponds to your fungi species.

Leave it in an area that is undisturbed, not too wet, you dont want any contam in the proximity of your Operation. Remeber that alot of contam is on the floor, alot airborne, always disinfect ur hands, any surfaces you are working on with Alcohol and any Equipment you use that directly Touches agar, liquid culture, spawn bag etc such as knifes and syringes should be Flame sterilized. Flame sterilization is best done with a "clean" Flame like from a butane torch. You can use a lighter to, the Black stuff "soot" thats left behind is completely sterile too so dont worry. Anything you flame sterilize should glow a bright Red, after Flame sterilization do NOT wipe down with alcohol, the thing you sterilized will go from sterile to sanitary (there is a slight difference)

But how? :)

@@shroomacademy4367 you can get small piece of metal into vial and use a rotating magnet at the bottom, it's very common in laboratories.

Magnetic stirrer? Sure we know that. The question is, how would you benefit from using it... :)

Ah I see what you mean. Still though, we're not sure what magnetic stirrer would do. It might interfere with the development of the mycelium, or it might help it. My guess is it would make no difference. But of course, its all just guesswork until someone does an experiment with a control group to check it. It would need to be a monoculture grown on the same batch of nutritious liquid media.

@@shroomacademy4367 I think it would be bad for mycelium is like an animal that has all of its stuff outside stirring it constantly would be damaging it

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There called vials

​@@hatsapp197 how to hit up !?

we take NaCl(or you can take 100ml Water for Injection)

Did you understand? I don't..

@@orion9k These are bottles of either sterile Normal Saline (water with 0.9% sodium chloride) or pure water for injection.

we have used all methods? but LC is the best!

I'd u put I. Fridge to lower temp it's gonna slow down growth a lot and can be kept for weeks or a couple months in not longer, as long as it's still looking good

1.5-2% better

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It's a basis for drugs that are administered via intravenous infusions in medicine. Saline solution can be used on its own for hydrating patients intravenously.

U can boil without pressure cooker

Yup, you just need to sterilize the substrate in a sufficiently large pot. The boiling should be for no longer than 20 minutes

@@shroomacademy4367 if i wanted to use 300ml mason jars instead what measurements of honey and water would i need and would the 20 minutes still be enough for that size

Having a chemical background we do know that a 100ml solution will not weight 100g. But it is close enough :) We found that particular proportion to be effective.

100ml H2O = 1801.528mol Honey (C6 H12 O6) = 180.1577g/mol 4% of 1801.528mol = 72.0611mol so we need 72.0611mol of honey which are about 0.4g. (keep in mind honey varies, so this is not 100% accurate)

Any of these will do: distilled water, tap water, saline solution

You can use plain fructose (not so difficult to get). We haven't tried sugar but we heard that it's better to avoid. Honey is most preferable for it's high fructose content as well as micronutrients

Yes, you can use jaggery/raw sugar or even plain sugar (dextrose). However the growth rate (since dextrose takes longer time to break down compared to fructose) will be slower thus it will take longer for the mycelium to form.

Never tried it, Tell us how it goes :)

Vegans 🥱

Honey has antibacterial properties that help out. I’d stick with honey

We don't think that antibacterial properties of honey are strong enough to make any difference. Not in such concentrations of honey anyway. However honey is preferable because it's much more rich in nutrients than plain fructose, which has no micronutrients whatsoever.

You can use corn syrup

it's not possible for any methods(((

I've read no one has successfully done with that mushroom

Yes, You should keep your honey percentage in distilled/sterilised water from 3-4% not more than that. For example you are using 100ml of distilled/sterilised water then you have to use 3-4 gm of honey. If you use more than that then it can might destroy your mycelium.

Yes, 4g for every 100mg of water, using oat based mixure cuts the dwell time like (dried or ground oats/ light malted yeast or ultra light M.Y.) mean it will start colonizing faster, using sugar based like honey, could be a couple week before u see growth in the grain

@@indibro1909 Is there a vegan option to use instead of honey? There should be, looking at the properties of honey, right?

@@Spasstiful You can Also use Light Malt Extract (LME) , but you should filter the solution two times using a coffee filter. 😇

@@indibro1909 thank you ✌️

yes

The culture can't breathe through vials, but it doesn't need to at this point.

We wouldn't post it if it wouldn't work in the end. While sterile techniques are always preferable, beginners can still can take advantage of this simple method before trying more advance things. Cheers.

I agree

@@shroomacademy4367 Not with you though shroom academy, lol. Just follow the tek.

Mushroom academy, you should post a video on how to make a contaminant free spore solution, then this video wouldn't seem like such horseshit. Creating a lc is the advanced thing that you're showing people how to do wrong. If you can't master agar, lc is out of your league.

@@trevorholliday7020 Actually LC is rather easier then agar, on our opinion. We used to do things a lot more strict in the past. Maybe we are just lucky but what we are doing actually works. But you are right, maybe we should make a video with a more strict adherence to aseptic practices.

Yes

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some people can't afford it. i tried this method and it worked! :D

If you store it in the fridge, then it's good for at least 6 months. We have successfully used a liquid culture which was much older than 12 months.

@@shroomacademy4367 For how many days can it be used at room tempreture. What is the temperature required for it to last for 12 months?🙏Reply

We never tested the shelf life in room temperature. I guess it would vary depending on fungi species that you use, but at a room temperature anything beyond several weeks is a wild guess.

The techniques described here can be applied to most mushrooms. Edible or otherwise.

As i understand it, you have to use the liquid mycelium to make mushroom spawn, with either grain, wood fibers or coffee waste. Then you can start growing the shrooms when you have a strong spawn going.

It may work, way higher risk of contamination, longer grow time and less yields are to be expected Edit Would only ever try this with gourmet mushrooms, dont do this with medicinal mushrooms itll probably go to waste

Look for the odd mycelium colors (i.e. that are not natural for mushroom species you are growing). You can do a test run on agar.

Its hard to Spot contam compared to agar. Always test the liquid culture for contam on a few agar plates or you might Ruin a fine grain bag/ whatever ur using with contam