Does Caramelization Effect Liquid Culture Growth? 

One of, if not the most informative mycology channels on YT. Every expirement is done presicely and backed by actual results. Not just regurgitating the same stuff we hear from everyone else.

this was straight up scientific method. legend gary as always

I too have caramelised my LC by mistake, there was no noticeable difference except for a less clear solution, we sterilise our LC for 25 minutes at 15psi. It works for us!!!

Great experiment . Thanks for sharing this. I almost threw my LC out recently when I saw the particulates,but decided to inoculate as an experiment too. And then here's your timely video, giving me this good news. Thanks Gary .

beautiful experiment bro, thank you very much for your time !

Great experiment...but a more interesting one would be with different medias like dextrose, fructose, corn syrup, honey, and malt extract just to name a few, and mix in some peptone to make it interesting. Thanks again for your content.....LC videos are major views.

Nice work, real science 🎉

Perfect timing. I was about to throw out a batch of LC because of caramelization, I'm definitely keeping it!

a sample size of "one" is not a truly scientific experiment. I PC my nutrient solutions for 35 to 45 minutes @15psi using clear, high fructose corn syrup. i never get solid caramelization, only a colour change. during my experiments with caramelization using honey, lme or dextrose I've found no concernable differences if caramelization happens or not in the 40 strains I have tested with a sample size of 6 with caramelization & 1 without caramelization of each of the 40 strains. we are all aware unless you are using 2 monokayorons to make your master culture you are getting many different genetics in a single culture which will lead to highly erratic experiment results. even using 2 monokayorons to make your master culture can lead to erratic experiment results because not every culture grows at the same rate in or on the same medium.

Agreed, I'v always figured since caramelization is just the burt sugars and protiens and the myco consumes both then its going to consume it reguardless of it's form, but i did note that some strains/species do really well in caramelized lc and some dont...bottem line..da mushys WILL grow in a caramelized LC period...by the way @Felix Merz..I copy and pasted ur "caramelization" cuz I was'nt sure how to spell it..😆🤣😅

Thanks for the info I was looking for this information because I had this exact problem not on a large scale like yourself, although I am trying to maximize my lions mane production so all the help I can get is appreciated. I’d really like to know how to make a tincture from different fungi do you have a video of that?

Such an important experiment for any grower, glad someone is talking the time to do it!

I do mine for fifteen mins in my PC. But i only do one or two jars at a time. When it's empty like that you don't need to leave it in for all that long i have found. Also i fill my water line to match almost match the water level inside the jars and that helps a lot.

Master Gary the one who taught me about turbidity!!! Always top notch mush love ❤️

have you tried to use your grain water for cultures and agar? I collected a bit of popcorn grain water from making spawn bags to run some side be side tests with 4% corn syrup solution.

Keep dropping these bangers🍄 this information makes my brain tingle🧠⚡️I think it but you do it❤.

I'm really liking the malt extract liquid culture I made it's faster than Karo ime

Don't forget, just like canning food....the more volume (bigger jars) are gonna require more time which could lead to more caramelization. Thanks, great video Gary.

What's the word on the morchella grow?

Thanks for doing this. I've caramelized accidentally and thought about throwing them out but gave them a shot anyways and they worked fine. The mycology community has a ton of these "an engine needs backpressure" type of old-wives tales and this is one of them. BTW, how do you attach the luer-lock filters to the lids? I've been using the filter stickers - one on each side - for LC and not really a fan. I haven't had problems but the luer-locks look like a more appropriate solution.

I love you Gary! Mush love!

Cubes love it

thanks for this information

Have you tried using glucose syrup?

Tha camera did you dirty brother.😅😅😅 looking a lil chunky in this vid

I am absolute beginner, this is my first LC but: I can use a snickers as "external stiring bar" because my Lion's Mane swims after it. Every other day it asks for KFC or some McD's. Fried chiccan AND caramelized topping for the icecream. Maybe brown rice instead of french fries - because it wants to remain lean. To still fit in a trunk. But no diet coke, yeah?!

Hey Gary! what is that pad you have in front of your flowhood @ 7:00?

As always great content Gary!!! Are your syringe filters 25mm? I have some 11mm, same size as the SHIPs. Thanks!! Mush ❤

Oh and btw, which jars are those smaller ones that im assuming are pints?

Hello how do you store the liquid culture jars ?

lc recipe question-Should i use karo and MEA or is this too much sugar?

My last dme lc I made I used allot of dme. It carmelized really bad. It was full of sediment. Within two weeks a point jar was half full of mycelium, it is chowing down on the sediment and fast. I always heard that allot of sediment would allow growth, but it didn't it grew really fast

Hey Garry. Great Video as always! Why don't you use Corn Syrup? Some people like it because the LC turns out very clear (if it doesn't caramelize).

What is that heat coil thing you're using to sterilize your scalpel between cultures?

Hi, im a beghinner grower i have problem whit liquid colture transparency , i see the acacia honey is more more clear of the bee honey and i wonder if is possible use plant honey and not bee honey , maybe is too contam? i can try filter normal honey liquid colture before sterilize it? Thk alot for sharing ur experience and the wonderfoul lab (sorry for my eng) . I try sob whit rise syrup and was a total fail of turbidity so now im scared make experiment

i accidentally carmalized my lc a few months ago but innoculated it anyway. its doin just fine. particulates make it look a little dirty but theres a lot of mycelium in the jar.

Hey I'm having some trouble with contams I've only been doing this for a couple months now can I get a little advice from you?

I would wonder if lack of clarity is more from protein breaking out of solution like happens with malt extract. I might try pre-boiling the mixture and then filter. This is what I have been doing with my malt extract before adding agar to get nice clear plates, and I could see it working here too.

thanks for this experiment, I was just getting ready to make some LC but using an instant pot. From what I see on YT they are saying 30 min is enough time. If anyone knows if that is correct Please reply. thanks

Do you prefer the Nylon syringe filter vs ptfe? I thought ptfe would be better because it is hydrophobic not really allowing moisture to permeate the membrane.

Which syringe filters you using?

that geeky sterilizer still flexin i see

i dont kno if any of you have seen yeast/fungi colonies that grow on top of mashes,, but they can form pretty cool stuff.

Sıvı karışımı neyle yapıyorsunuz ve kaç günde hazır oluyor

What kind of blender do you use!

This is a good one I think there's plenty more myths to bust in mycology to

Gary looking a little rich there buddy! Or it's the camera angle.

Affect ;-; love your videos tho

Dont use fructose (in general imo, plenty alternatives).. I think can also help to avoid mixing too much nitrogen compounds like amino acids (altho temp should be too low for Maillard reactions) and ofc malt extract can have its own problems, i think prob because some of the compounds present are just *barely soluble. In general in chem, very tiny amounts of impurity can discolor a product... In which case it would not be likely to have much effect. Also bit more richness in chemical variety might keep more different enzymes busy.

🪄

very nice