🔬 How to do focus stacking | Amateur Microscopy

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Focus stacking combines several images of a different focus into one final image that has all parts in focus. Here I show you how to do this using stereo microscopy.
Focus stacking with Picolay: • 🔬 Quick Focus stacking...
Camera used: Summit SK2-5.2X 5.0MP PC/MAC
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🔬 How to do focus stacking | Amateur Microscopy

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15 авг 2020

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Комментарии : 33

@MicroscopyShorts 24 дня назад

Literally every time I look up a new microscopy technique I find one of your friendly videos, have a good day!

@MicroAddict1 20 дней назад

nice video I used Picolay many years ago when I was working as a naturalist at a local salt marsh nature center, performing many microscope classes and training sessions - it worked very well

@DB2023Space День назад

Thank you, loved the video and the process! With something such as a focus motor control, you could make it more consistent.

@THATHATSALLFOLKS 3 года назад

HELICON FOCUS!

@niramaygogate7904 3 года назад

Hey, nice video. I had tried image stacking for astrophotography. I had a good feeling that it could be used in microscopy as well. Thanks for the clarification : )

@emmanuelblieck4826 3 года назад

excellent tutorial. Thank you

@akosszilagyi4504 3 года назад

Thank you Oliver!

@bradxwx 3 года назад

good job with this channel. my microscope arrived and I (mostly) knew what to do to get started. bout to try stacking some photos I made

@TenesseeTuxedo1 2 года назад

Great stuff

@Spartan1-1 3 года назад

Can you make a video on gooseneck illuminaters?

@johnrobinson4445 3 года назад

This is my next challenge.

@MageMath 3 года назад

How did you get the blueish background behind the wasp? Thanks for the great video.

@MicrobehunterMicroscopy 3 года назад

It's actually a white piece of paper that looks blue because of the low light.

@christopherbivins7459 3 месяца назад

Hi, great video! I was wondering if you know of any other options that can achieve a similar result for the Digital Photo Professional 4 software? I use a Canon Rebel d6 attached to my microscope and have been exploring different options for photo stacking. I was looking at WeMacro's micromate option for automating adjustment of the focus knobs on my microscopes, but then I found this video and this seems like a much cheaper and easier option. However, I can't seem to find a similar feature in DPP4 that allows an continuous capture process like the one you demonstrated here with the Time-lapse (auto capture) feature you demonstrate in ToupView. I've also tried finding a way to install ToupView, but it seems like the only option to download it on my mac is from the Apple Store where it says it is designed for iPad. Long story short, I'm looking for a similar feature in DPP4. Do you know if that exists and if so, how to utilize it?

@glasslinger 3 года назад

Too many pictures. Sometimes as few as 5 from the list (every fifth picture) will give better results than 60 images! I have never seen where over 20 images are needed. Some of the best microscope images on the net were made with only 5 frames combined. 10 to 20 seems to be the sweet spot. Very nice video to help getting started.

@gunner0446 3 года назад

can you please help me with toupview. I see when you click on exposer, that a green box appears, just like the one in your video. would you know why do I NOT see this box come up. I'm trying to get it to work on white balance, but it don't show up for me. the only time I ever see any kind of box is if I go up to the top menu bar, and click on grey calibration. that is the only spot in the entire program where I do get a box to appear for calibration. I am using the latest version of ToupView, and ZZcat 5.0MP camera, SCMOS05000KPA usb 2.0 and 1/2.5" aptina cmos I am new to microscopy, and I just want to learn... thank you. and keep them videos of your coming, I love watching many of these.

@darylwhelan7966 2 года назад

hello is the combine zp apart of the toup app or is it a free app somewhere else

@whalebone01 Год назад

Hi,, The Toupview program I downloaded will not detect my camera??

@nyksiex 3 года назад

Which one is better, sw380b or sw380t? And do they have darkfield, phrase contrast etc.?

@MicrobehunterMicroscopy 3 года назад

the b is binocular and the t has a phototube. So this is better for taking pictures. They do NOT have phase constrast (this would be much more expensive and other brands) and darkfield can be done by making a filter yourself.

@C-by 3 года назад

hy I'm from Indonesia. I would like to ask you. what microscope lens (objective) is suitable for macro photography?

@MicrobehunterMicroscopy 3 года назад

For macro you have two options: get a macro photography lens (lower magnifications) or a stereo microscope (higher magnifications). For photomicrography you need a compound microscope (still higher magnification of transparent objects)

@burtonkeeble670 3 года назад

I'm trying to download CombineZP. What I keep getting is File name: combinezp.msi, which is a word document. How can I get the program for stacking? btw, I had no trouble getting the Picolay installation program.

@MicrobehunterMicroscopy 3 года назад

the msi document is actually an installation file. Word should not open it.

@burtonkeeble670 3 года назад

@@MicrobehunterMicroscopy -- Thank you Oliver. It downloaded as "combineZP.msi.doc", which seemed quite strange. Word *did* open the file, which was mostly gibberish. :D I've got it working fine, now. ;)