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2.9 Separation of Photosynthetic Pigments by Chromatography (Practical 4) 

Stephanie Castle
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25 авг 2024

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Комментарии : 60   
@rondayaram5792
@rondayaram5792 3 года назад
Incredibly detailed. Well done. Thanks for sharing
@nguyenkienam2331
@nguyenkienam2331 3 года назад
this helped me a lot in lab mixture separation report
@37KA4
@37KA4 3 года назад
Hi please can I contact you via email ..
@anantmalik17
@anantmalik17 Месяц назад
Absolutely helpful and on the point. Thanks. My lab practical is on it's way. It helped a lot
@breeze3430
@breeze3430 2 года назад
Thank you! This is a helpful guide for our Plant physiology laboratory activity
@teacherjayzeelynvanessacor6889
9 ⁹k
@drewhenderson8223
@drewhenderson8223 10 месяцев назад
Your welcome
@idkhahahaha
@idkhahahaha Год назад
but what does the Rf value actually tell you about the pigment?
@DeepikaSharma-io7bl
@DeepikaSharma-io7bl 2 года назад
Well done mam it's really very helpful video for us keep create video for us
@deminigamage9438
@deminigamage9438 2 года назад
Thank you so much! I didn't understand anything given in my lab manual. But this is very clear.
@peneloppedjeakoua1570
@peneloppedjeakoua1570 9 месяцев назад
Very useful for my experiment tomorrow
@Kwork1104
@Kwork1104 Год назад
Helpful video Must all bsc students watch this before exam❤❤
@phenyotshabang6394
@phenyotshabang6394 3 года назад
Can someone please explain why acid washed sand and anhydrous MgSO4 are used in the preparation of the extract of this kind of chromatography.
@hotpepper7782
@hotpepper7782 3 года назад
Well my bio teacher said the acid washed sand was just there to make the grinding process easier about the anhydrous MgSO4 idk y it's there.
@kennyyong7694
@kennyyong7694 4 года назад
Excuse me, it is the same procedure for thin layer chromatography right?
@Palak..15
@Palak..15 10 месяцев назад
Yup:)
@sabiha23467
@sabiha23467 Год назад
Thank you madam I understand very well
@khushiparmar7444
@khushiparmar7444 6 месяцев назад
Solvent system which solution use?? Can you please tell me
@misskhan8328
@misskhan8328 2 года назад
your english is too good
@niloypaul34
@niloypaul34 7 месяцев назад
thank you
@shristiasylum
@shristiasylum 3 года назад
Good job dear👍
@climax7260
@climax7260 3 года назад
Thank you it helps
@TheHexbugfan
@TheHexbugfan 4 года назад
Ms. Webber sends her hellos!
@pawsitivebhumika6462
@pawsitivebhumika6462 3 года назад
Thanku so so much❤
@zmazn
@zmazn Год назад
what should I do or what does it mean if the carotene keeps ending up being the last one below? I tried it 2 times and its always the same and im afraid the school lab wont let me do it again and i have to make a lab report about it with my picture in it.. I used spinach leaves btw
@siddikpk8385
@siddikpk8385 3 года назад
😊 🙏 thanks
@sashaart781
@sashaart781 2 года назад
Can I ask what solvent did she use? Please answer me 🙏
@ayodelebello1145
@ayodelebello1145 4 года назад
How much acetone is used for each leaf sample?
@detss3795
@detss3795 4 года назад
not just acetone 5 ml solvent which contain 9 part Petroleum ether and 1 part acetone
@detss3795
@detss3795 4 года назад
@Elijah Tam-od Ok
@roshnimathew3209
@roshnimathew3209 2 года назад
Instead of ether which solvent can be used?
@Idk-ij1yy
@Idk-ij1yy 2 года назад
Is there any way to improve this method
@afolabifakanbi7782
@afolabifakanbi7782 3 года назад
Tanks
@Leen-ih8ql
@Leen-ih8ql 3 года назад
what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help
@videoteaching47
@videoteaching47 2 года назад
u can do through chemical method as well.
@Anki1243
@Anki1243 4 года назад
Which solvent used?
@kiransharma-oo2wq
@kiransharma-oo2wq 4 года назад
I think acetone
@detss3795
@detss3795 4 года назад
5 ml solvent 9 part petroleum and 1 part acetone sis
@roshnimathew3209
@roshnimathew3209 2 года назад
Instead of ether which solvent can be used?
@anishakumarisingh581
@anishakumarisingh581 3 года назад
Plss give me the exact amount of solvent
@geetaikalpande8471
@geetaikalpande8471 2 года назад
AND WHAT HAS SHE ADDED???
@evelynt.britos2863
@evelynt.britos2863 4 года назад
What’s the significance and use of Rf value?
@evelynt.britos2863
@evelynt.britos2863 4 года назад
After determining Rf, what’s next? Does it end in finding the Rf? Thanks.
@martajackson11
@martajackson11 4 года назад
Hi, biology student here! From what I know, the Rf value is one of the methods you have to distinguish between the several photosynthetic pigments you can find on a leaf. You can also identify the pigments just by looking at their color. Basically if you have a Rf value close to 1, that means that the pigment moved quite a lot and it was abel to do that since it a polar pigment. If on the contrary you have a low Rf value, that means that the pigment didn't move that much and thus has non polar groups in its constitution. Please correct me if I am wrong
@xyrildanmanuel783
@xyrildanmanuel783 2 года назад
hi chemistry student here. the Rf value is one of the information we use to pinpoint what the pigment might potentially be. chemists around the world do this chromatography on initially known compounds and they share and confirm the Rf values they have obtained in a database (or multiple databases like the one presented at the end of the video) so that future analysts would have a reference when repeating the same chromatography on the same compound. think of it like a pseudo-fingerprint of a certain compound although i believe not all compounds have unique Rf values
@ahappycoder2925
@ahappycoder2925 2 года назад
RF value is used to identify the pigment
@jimsamson7934
@jimsamson7934 Год назад
It allows you to get a measurement for 5G or 3G radiation frequencies. That way, you can gauge how thick your tinfoil needs to be in order to block those signals
@daphnesaipelekomiti7254
@daphnesaipelekomiti7254 3 года назад
Can someone please my lab
@judeigho7254
@judeigho7254 3 года назад
Is the eluting solvent polar of non polar?
@ammarsyah267
@ammarsyah267 10 месяцев назад
thanks .. "a few drops of acetone" 🗿
@realism6793
@realism6793 7 месяцев назад
wat the pigments i got 10 mins
@randomgaming9869
@randomgaming9869 3 года назад
Result
@keerthicreations2153
@keerthicreations2153 4 года назад
Hii
@keerthicreations2153
@keerthicreations2153 4 года назад
FF
@keerthicreations2153
@keerthicreations2153 4 года назад
FF
@keerthicreations2153
@keerthicreations2153 4 года назад
FFFFFFFFFFFFFFF
@detss3795
@detss3795 4 года назад
@@keerthicreations2153 hi yes What u want to ask
@ranaumararshad3351
@ranaumararshad3351 8 месяцев назад
Tare pan nu lun
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