Performing Thin Layer Chromatography (TLC)

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We've learned a few separation techniques, so how about one more? Chromatography separates components of a mixture by virtue of their differing polarities, and thin layer chromatography, or TLC, is an invaluable technique that is used almost every day by any laboratory chemist. Let's run through the details of this indispensable tool!
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Опубликовано:

16 фев 2023

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Комментарии : 111

@the_most_stable_alkene6666 Год назад

As a research chemist tlc is an important step in determining a reactions success. I actually learned tlc from your channel back a few years ago

@jameshahollomon4893 9 месяцев назад

THANK YOU SO MUCH! This is way more helpful than reading a lot of techniques from the lab manual!

@SaadAli-qv9nf 6 месяцев назад

Thank you so much Mr. Dave for explaining so well and simple.

@syruponcereal Год назад

Great timing. I’m doing this lab next week

@arpita5517 5 месяцев назад

im watching your videos during my JEE prep to get better clarity for certain topics…thank you professor!! much love from india :)

@anubhutisingh5920 3 месяца назад

I'm here for neet as well😂

@mohammadmanhar8839 Месяц назад

@@anubhutisingh5920 Get it on guys 💪

@heavenlysongs7307 Год назад

Yesterday was my chemistry practical exam And I did chromatography very well

@RashedMA_TKO Год назад

Man ur such a god send. I literally have a TLC write up due tomorrow

@veronicaaguiniga8496 Месяц назад

As someone who has already performed multiple TLC experiments, I feel that this video will definitely help students who want to watch how to perform this experiment before actually doing it in class. This has the upside of the student focusing on the understanding part instead of focusing on how to simply perform the experiment. I also appreciate that this youtube channel highly recommends watching an in-depth video of TLC before watching how to perform it in lab.

@kaushikumarihami1982 8 месяцев назад

marvelous!!! simple, but has all the elements needed

@salmonorange Год назад

Great video! Hope you can teach us how to use different stains to find out compounds that cannot be seen under the UV light.

@seyedmostafahosseipourmash9168 3 месяца назад

Perfect and very simple while teaches all necessary info

@user-nj4rc5qn3y 9 месяцев назад

amazing video, thank you professor

@holgerharild1638 Год назад

Very good and easy to understand

@Thaumius Год назад

In addition, if you wish to reuse a capillary you can dip it in acetone to clean it between spottings

@s.9417 2 месяца назад

What about ethanol or methanol instead?

@dharanireddy1806 7 месяцев назад

you are awesome professor Dave thank you for this video

@henna_nenna 4 месяца назад

oh my god you're actually my savior thank you

@shereea1823 Год назад

Another fantastic video loving this series

@niloofarkh4779 2 месяца назад

thank u so much , it is perfect

@hatlessjet7802 Год назад

I love these videos. Thank you. This helps to understand things a lot. I found out that j tolan lied about how far away he was on the 1000 mile video.

@bendfocuspro Год назад

These are great. I am a sys admin for a lab and these videos allow me to ask informed questions of the chemists that work there.

@vidcoyoyo3156 Год назад

TLC can be used quantatively by using different concentrations of the same standard, and comparing the sample spot size to the standards.

@sherlackholmesholmes8374 Год назад

Thank you sir

@apoenzyme8085 2 месяца назад

Great job man

@beverlylapierre9894 2 месяца назад

SO HELPFUL!!!!

@mahdinajm9310 10 месяцев назад

Keep the good work

@qzh00k Год назад

Worked for the Jarrel-Ash division of fisher scientific for a bit. OES is interesting

@apurva845 Год назад

Reminds me of my Botany practical in college

@Bludgeoned2DEATH2 Год назад

Can’t wait to get to HPLC and GC, my specialty 😊

@mahmoudsanad6447 Год назад

Excellent, but you should make many examples for differnt sustances,polar or non polar,in addtion you can explain column chromat. and HPLC.

@theh4sh 7 месяцев назад

Thank youu professor

@KJT_izumi Год назад

0:29 Slight glitch in the edit there! The intro popped in there for a couple frames. ...But it's something that doesn't take anything away from the video at all. Good work! :)

@M4RC90 Год назад

And at 6:28 I thought my GPU was dying.

@ridhafaizal2620 8 месяцев назад

Very useful👍

@pranavsingh5235 3 месяца назад

thanks ❤❤❤

@user-oq7kn8py4d Год назад

You are great❤

@redvelvet3479 11 месяцев назад

I have a qustion. What if the extract sample we have is semi-solid, how do we dissolve it? and how to choose the appropriate solvent for a sample that has never been studied?

@marizarm 3 месяца назад

thank you so much, it helped me a lot!!!

@jpnye Месяц назад

Anybody know why the jar needed to be shut? I assume to prevent evaporation during the experiment, but I was wondering if there was a more specific reason. Thanks for the great video!

@sciencenerd7639 Год назад

good ol' thin layer chromatography thanks!

@Akaeru 7 месяцев назад

What solvent would you need for flavonoids from cabbages, professor Dave?

@KilledInUkraine Год назад

great video, hope you do one one column chromatography (adsorption or flash chromatography) and explain how tlc can be used to effectively create an efficient solvent system for any purification work-up using the more common column chromatography. Can a standard UV-C light (~253nm, for disinfecting) be used for visualizing plates at home? they are only like $10, compared to the mineral lights or light boxes used in labs which are hundreds of dollars. when creating reference samples for the tlc spotting of known or unknown samples, do they have to be in aqueous solution or in a solvent, and does it matter how dilute the sample is? How do you create an Iodine chamber for developing tlc plates?

@_soyel786 3 месяца назад

My teacher told me to watch this video 😅

@munavathtanuja4401 2 месяца назад

😅😅😅

@Joemamahahahaha821 Год назад

After Jesus turned water into wine he did a TLC to test its polarity against a store bought wine.

@fukpoeslaw3613 Год назад

That's not in the Bible! You're probably an atheist.

@keypo790 Год назад

lmao

@atifashhabatif8391 Год назад

💀💀💀💀💀

@whydidtheballooneatthefox282 Год назад

😂😂

@notveryrea1 Год назад

🤣🤣

@draganmirkovic9377 Год назад

Thanx, can You show elisa?

@rassimsimou1594 Год назад

Good

@jenniferwinkel4588 Год назад

Hi!! I was wondering why you should cap the chamber for a few minutes before using it? Is there a specific reason for that?

@mnemonicallysathyan4339 8 месяцев назад

IT WILL EVAPORATE TO THE SURROUNDINGS

@hanniehmar8885 Год назад

Ahh fisher my number 1 MSDS source

@infinitivez Год назад

What's the purpose of capping the solvent and letting it sit, before introducing the plate?

@colinprice712 Год назад

Letting the tlc tank become saturated with the solvent vapour means that the solvent doesn’t evaporate from the leading edge as it rises up. This slows down the rate of movement and the components of the mixture separate into better defined spots rather than streaks. There are some times when this isn’t needed, so you can run the separation without tank equilibration. Also, many of the solvent mixes used are not “nice” chemicals to have in the air…

@waelfadlallah8939 Год назад

Thermofischer is my favorite chemicals supplier. In my lab setting i always order from them, they provide the highest quality amongst the different chemicals that i work with.

@waelfadlallah8939 Год назад

@@bugsbunny852 funny bunny 🐰 😆

@ProbusMihraban Год назад

Cool

@hananl.3147 8 месяцев назад

Is that a fly in the solvent?

@Danthebestman Год назад

My guy you still make videos??? You are a determined man Respect

@Sidistic_Atheist Год назад

Why couldn't you have been my Chemistry teacher 45 years ago.. All this made total sense to me. Proof, that there is NO such things as, bad students, only bad teachers.

@jwl796 6 месяцев назад

Nice video!! I was so confused when I was doing this lab😢

@heehee4454 Год назад

thank you chemistry jesus

@LucasPlay171 5 дней назад

Yo i could maybe use this to test for NBOMe/LSD/something else, cool

@ilmacademy5735 Год назад

Sir among Be and Ga which is more metallic element

@ilmacademy5735 Год назад

@@bugsbunny852 yes

@ilmacademy5735 Год назад

@@bugsbunny852 i know the answe...but list night a professor said something different...that is why....nd mind ur own business.....

@-JA- Год назад

👍👏

@glennpearson9348 Год назад

How does this experiment work when gravity doesn't exist? LOL! Just kidding! I'm enjoying these practical lab videos. They're taking me back to my organic and inorganic chemistry days in college.