48h Phase Contrast Live Cell Imaging of Epithelial MDCK cells 

Watch these happy healthy cells grow and proliferate.
Epithelial MDCK (Madin Darby Canine Kidney) cells were cultivated and imaged for 48h under physiological conditions.
Material and Methods:
Passage 12 MDCK cells were cultured in DMEM supplemented with 10% fetal calf serum (FCS) and seeded in the ibidi µ-Slide VI 0.4 (ibidi.com/chan....
Phase contrast, time lapse microscopy was performed on a Nikon Eclipse TiE inverted microscope with 20x objective for 48h with a time intervall of 10min and a total of 289 frames. Temperature (37°C) and Humidity (75%) were controlled using the ibidi Stage Top Incubator (ibidi.com/78-s....
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