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Epithelial MDCK (Madin Darby Canine Kidney) cells were cultivated and imaged for 48h under physiological conditions.
Material and Methods:
Passage 12 MDCK cells were cultured in DMEM supplemented with 10% fetal calf serum (FCS) and seeded in the ibidi µ-Slide VI 0.4 (ibidi.com/chan....
Phase contrast, time lapse microscopy was performed on a Nikon Eclipse TiE inverted microscope with 20x objective for 48h with a time intervall of 10min and a total of 289 frames. Temperature (37°C) and Humidity (75%) were controlled using the ibidi Stage Top Incubator (ibidi.com/78-s....
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29 авг 2024