Definition
different forms of the same enzyme catalyzing same reaction
How they are formed
1. Different genes- true isozymes
2. all variants are present in same individual
3. isoforms- due to post translational modification
4. allozymes- from different alleles
characteristics
1. electrophoretic mobility
2. substrate specificity and Km
3. Heat stability
4. Inhibitor
5. Coenzymes
6. Antibody sensitivity
7. Tissue of origin
examples
LDH- 1 to 5
CK 1 to 3
ALP-6
clinical significance
24 сен 2024