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Acid Fast Bacteria (AFB) Stain Kit (Microorganism Stain) (ab150660)，It is not heat fixation after carbol fuschin staining. Could you please explain this method.
Thankyou da for this beautiful demonstration. I am curious to know why this process of Acid fast staining is named so? And one more thing daa, which tool do you use to prepare your ppt of this kind where you get cartoon like beautiful images?
Acid-fastness is a physical property of certain bacteria, specifically their resistance to decolorization by acids during staining procedures. Once stained, these organisms resist dilute acid and or ethanol based decolorization procedures common in many staining protocols. Thus, the name ‘acid fast’ is given to those organisms.
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You mentioned heat fixation after carbol fuschin staining. Is heat fixation always done after primary staining or heat fixation is done first followed by primary staining and so on just like other staining procedures?
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You mention that acid fast bacteria do not take gram stain. However, are all acid fast bacteria gram positive? This was something that confused me in the past. They often appear gram negative.
Acid-fast bacteria could be gram-positive in terms of structure because they contain peptidoglycan in the cell wall. However, they stain poorly with Gram stain procedure, appearing weakly Gram-positive.
@@animatedbiologywitharpan OK but are ALL acid fast gram positive or just some of them? I don't even know if this is a known fact or not but thought I would ask you out of all the people on the planet.
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