Four Quadrant Streak procedure - How to properly streak a Petri plate for isolated colonies

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In this video you will learn how to use proper lab technique to streak a Petri plate for isolated colonies using the four quadrant streak method. To learn more about Hardy Diagnostics visit our new website. 👉 go.hardydiagnostics.com/yt-ha...
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00:00 Intro to streaking an agar plate
00:21 What to know before beginning
01:01 Preparation
01:50 Four quadrant streak diagram
02:29 Types of loops
03:28 Collecting a sample
03:43 How to do a four Quadrant Streak
04:36 Using a swab
05:28 Incubating the plate
05:43 Using a plastic loop
06:24 Close and ordering info
Do you prefer reading when learning? Here is our blog:
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Filmed and Edited by Brian Tom
#Streakplate #Bacteria #Microbiology #HardyDiagnostics #4quadrant
Tags:
quadrant streak plate, streaking agar plate, agar plates, streaking agar plates, quadrant streaking, how to streak on agar, what is a four quadrant streak, four quad streak, how to streak a petri plate, petri plate streaking, streak plate method english, quadrant streak, spread plate technique, streek agar plate, what is a four quadrant streak, what is a four quad streak, help doing a four quadrant streak, study four quadrant streak, exam four quadrant streak

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28 май 2024

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Комментарии : 108

@Ms1101997 Год назад

I’m a new microbiology student and I’ll do this for my tomorrow’s lab 😅 I was confused but thanks god I found this video

@HardyDx Год назад

We love helping new micro enthusiasts! Feel free to browse our other tutorials for your class too!

@winispecial Год назад

"Thank God you found this video "

@CrankyRayy Год назад

@@winispecial " " Thank God you found this video " "

@mdraihan1582 2 месяца назад

What's your country name?

@Ms1101997 2 месяца назад

@@mdraihan1582 I’m from the US, California

@notjeb_9409 Год назад

its so wild how a lot of ppl commenting here r college students or professionals n here i am watching this for our lab activity in my high school biology class. cool video, helps a lot

@nina-ge6mn Год назад

I love microbiology and honestly its just so satisfying to watch people streak plate I'm doing a microbiology project that involves e. Coli K-12 plating and im so excited

@HardyDx Год назад

Nina, we love projects for the tiny humans! Please feel free to reach out to your local sales rep for product, we'd love to help out the next generation of micro-microbiologists!

@Faceless_Chat Месяц назад

Very helpful. I have a "unknown bacterium" assignment that starts today. This really helped bc I already forgot you have to sterilize the loop between streaking...

@daliadivicpuskarski7453 Год назад

Yesteday I streaked a Petri dish for the first time in my life. I've watched this video before doing it and it really helped me 🧫 Thank you 😊

@HardyDx Год назад

That's wonderful. Glad it helped!

@sloane.2747 Год назад

This was so much fun when I did it, microbiology was my literal favorite class my first semester of college

@HardyDx Год назад

Glad to hear it! Keep on learning!

@clairem290 Год назад

I'm a college instructor and I'm sending this video to my students before they do their first-ever cultures!

@HardyDx Год назад

We love our instructors and our students! Feel free to reach out to us should you need materials for your students as well!

@Flowers12660 3 месяца назад

Well as an instructor why don't you show your students this yourself? Isn't this supposed to be your job to teach? It would be alot better. I really dislike and hate how college so called instructors and lecturers are relying so much on youtube videos instead of doing the work themselves.

@clairem290 3 месяца назад

I did streak some plates myself, which came in handy later. However, a nice RU-vid video they could watch the night before was also really helpful. To make up for your previous instructors who didn't explicitly show you enough things, I will now show you how to spell the phrases "so-called" and "a lot."

@africachamber6596 19 дней назад

thank you for saving my ee broski 🙏🙏

@jessical4866 Год назад

I’m not a teacher or a student… why am I so fascinated with this? 😂

@HardyDx Год назад

I mean we can't blame you, we do this for a living. Our whole lives are this, welcome to the obsession!

@GaneshKunayak Год назад

Who are you 🤣😂

@affiliatemarketinginnigeri7617 Год назад

I'm a passer-by as well and I am entranced ❤️

@DanTheisen 2 года назад

This is an excellent video. Thanks, Hardy for showing us how this is properly done. Much more to it that a squiggle of bugs on a petri plate!

@HardyDx 2 года назад

Glad it helped!

@DanTheisen 2 года назад

As required by law, I’m disclosing that I was employed by Hardy Diagnostics at the time of this comment.

@Sean-ur4cb Год назад

@@DanTheisen what did you sign that told you to disclose this info?

@DanTheisen Год назад

I don’t recall where I saw it but this is, as far as I know, meant to prevent large companies from having their employees promote their products as if they are customers. I have not signed anything from the company compelling either a comment or a disclaimer about the comment. The law as I read it made sense to me from a consumer protection point of view, which is why I happily added it.

@Sean-ur4cb Год назад

@@DanTheisen ahh I see, thanks for the reply. Have a good day!

@johnson1262 Год назад

My universities professor , didn’t tech to put the petri disk , up side down when going into incubator. Thank you for sharing.

@HardyDx Год назад

Oh no! When media is incubated "upside down" like that, with the media facing upwards, condensation can accumulate on the face of the agar and inhibit proper growth and developing colonies. If you invert your plates, you'll definitely have better-looking plates than the rest of your class!

@Dananq98 Год назад

So useful description, thanks a lot 👏🏻

@HardyDx Год назад

Glad it was helpful!

@AL_ayyubi1137 Год назад

I'm medical laboratory technician(MLT) and 11 lecture is included for simple technique in tomorrow thank you for this video I appreciate it 🤩

@HardyDx Год назад

You are welcome, I am glad the video was helpful. Keep up the great work as a MLT. You are important.

@user-ou9kq6xf7k 3 месяца назад

It's self explanatory and the visuals, helps for easy remembrance. Thanks.

@HardyDx 3 месяца назад

You are very welcome. I am glad our video was useful for you.

@leannajulien4175 Год назад

I didn't know I have place it in the heat everything I learned something new. Thanks

@HardyDx Год назад

We're always here to help you learn! We have a great Technical Services team should you ever have questions!

@KHALILLABSKILLS Год назад

I really appreciate you sir.

@ayesha-it5yw Год назад

I learnt this method today in my microbiology lab.

@HardyDx Год назад

That's great Ayesha! Keep up the proper four quadrant streak method, you will be a pro in no time!

@ruchika_899 Год назад

Gorgeous explanation 💥❤️

@HardyDx Год назад

Thank you, glad you liked it!

@anycamenzind 6 месяцев назад

Will there be a video available that talks about the microscope and Khoeler illumination? Unfortunately, part of the practical microbiology and laboratory sessions were taken in times of pandemic. Likewise, I would greatly appreciate if you could explain how the UFC colony counter works. Your explanations are great! Thank you 🙌🙌🙌

@tayyabirshad3029 Год назад

Today may microbiology practical exam it's help alot!!🥰🥰🥰🥰

@HardyDx Год назад

Glad to hear the video was useful. You are very welcome. Good luck with your exam. You will do great!

@jennisagala1263 Год назад

thanks a lot, i hope, my thesis will be finisih

@KHALILLABSKILLS Год назад

It's absolutely Amazing sir.

@HardyDx Год назад

Thanks, it's our pleasure.

@Manthab Год назад

Don’t know how I got here at 1am but it was fascinating and informative if I ever need to know this I’m covered😁

@HardyDx Год назад

Welcome down the rabbit hole of microbiology, Mantha! We've got a whole channel of videos if you ever need more obscure information to get you even more hooked 😃!

@ruchika_899 Год назад

Excellent explanation ❤

@HardyDx Год назад

I am glad our video was useful for to you.

@KHALILLABSKILLS Год назад

It's well explained.wow

@vennelagovindugari5420 2 года назад

Perfect!!

@todddillerson4053 Год назад

I do this every day for the hospital I work at :)

@HardyDx Год назад

That's great! Heroes wear lab coats not capes. Which hospital do you work at?

@Hellamoody Год назад

@@HardyDx boundaries Dwight!

@nithinmuluka7987 Год назад

Thank you sir very nice video... Pls upload more videos

@HardyDx Год назад

Yes, stay tuned. More video are on the way!

@1alex92 Год назад

Wish I seen this 2 weeks ago when I had a test! ...nevertheless.. I just subscribed ☺️

@HardyDx Год назад

Welcome to the online Hardy family! Stay tuned for more great content soon to come!

@AliyuNanaaishat Месяц назад

Please can I see a video that will teach me more on how to carry out indoor air quality using SDA and PDA

@apokalypthoapokalypsys9573 Год назад

I'm in an IT MSc but this is perfect ASMR

@HardyDx Год назад

Love it! That's a whole new industry we should break into!

@user-ot7km4px5n 6 месяцев назад

How do you report colony growth on a 4 way streaking? along with its size, shape, transparency, consistency, and color.. Thanks

@Szaisthatyou Год назад

I love my major ❤

@FarzadSalim Год назад

thank you

@HardyDx Год назад

You're welcome.

@RaiSaysS 2 года назад

Thank you

@HardyDx 2 года назад

You're welcome.

@nangsanbhalangblah333 Год назад

Great vid, I've a question though. Why is the media red in color? Is there a different type of agar used?

@918mounika7 Год назад

It's probably blood agar medium, blood added to regular nutrient agar medium

@HardyDx Год назад

Nangsanbhalang Blah, Great question about the coloration of the agar! This was streaked on our Blood Agar, an all-purpose growth agar with hemolytic properties. Some organisms will hemolyze the blood in the agar, leaving clear zones around the colonies, something we won't see on traditional TSA. Hope that helps!

@ceciliaovansa2738 3 месяца назад

Nice one

@HardyDx 3 месяца назад

Thanks for watching

@joannadevung4358 Месяц назад

If i accidentally tore the surface of the agar using the loop during streaking, will the bacteria colonies still able to grow?

@HardyDx 15 дней назад

Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

@sunny4483 Год назад

Can we not streak circular on plate instead of streaking zig zag? I'm curious

@MrDubbindan 7 месяцев назад

There is an instrument that does streak with a plastic "comb" after pipetting a volume on a plate and it does make circles ... The Previ Isola

@user-bw1uz7oy1w 6 месяцев назад

Quand utiliser la methode en stries?

@pastmonster446 Год назад

Watching this after I took my lab practical exam, and I definitely fucked it up

@mohanzm12 Год назад

What do we call the thing she used to flame the loop ? 😅

@DadaClaire Год назад

I'm curious too. I'm working with unicellular fungi and bacteria since time, streaking them to isolate but it's the first time ever I'm seeing that instrument.

@vanessadang4674 Год назад

It is called a bacticinerator :)

@DadaClaire Год назад

@@vanessadang4674 Thank you!

@HardyDx Год назад

@Mohan The tube the loop was inserted into is a special type of sterilizer. Placing non-sterile metal loops into this tube for a few seconds will sterilize these loops so you can re-use these again and again. Hope that answers your questions!

@mohanzm12 Год назад

@@vanessadang4674 wow .thank you

@GaneshKunayak Год назад

Bdw i think this must be carried out inside a laminar air flow

@chrisk6469 Год назад

Watching them tear up the agar like that was a bit painful ngl

@hebahariri9936 11 месяцев назад

Hello, I'm a microbiologist and a content creator on Instagram educating microbiologists in Arabic, can I translate your video and post it on my account? with sharing your video link of course. thank you.

@HardyDx 11 месяцев назад

Yes, that would be fine as long you tag and credit Hardy Diagnostics in your video description and link it back to the original video. Keep up the great work in microbiology.

@M313_1 3 месяца назад

*ثانكيو* 🦦

@akritikuikel8506 7 месяцев назад

how to get a job or internship of microbiology in europe. I have done bachelor in microbiology and working in a restaurant since 4 years. Did not even get any inernship. Already forgot all the techniques and skills in these 4 years . sad sad

@BlueFox61 Месяц назад

Build a portfolio of your interests and maybe your thesis paper too. It's hard to get an internship now

@SOPHIA-yf7ws 8 месяцев назад

A++++

@lilianmumbo880 2 года назад

5 method of preparing specimen

@kasisfile Год назад

😮

@alvinxyz7419 Год назад

I thought the title said steak

@sriningsih4808 Год назад

kangen haha

@user-me1hh5po6h Год назад

عربي وين

@Behejrbrbrjrj Год назад

هنا موجودين 🙂🙂

@John-fr6wo Год назад

For God so loved the world that He gave His one & only Son, that whoever believes in him shall not perish but have eternal life. For God did not send His Son into the world to condemn the world, But to save the world through Him. (John 3:16-17)✝️