Agarose Gel Electrophoresis

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This video demonstrates how to load and run DNA samples on an agarose gel. Basic information about the charge of DNA and how it will run in an horizontal electrophoresis cell is explained.
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Опубликовано:

10 окт 2012

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Комментарии : 252

@waowwaow8239 Год назад

Hop Barış hocamdan geldik buradayız

@kerimbeyazt4175 8 месяцев назад

barış hoca da olmasa geleneksel eğitim metotlarına maruz kalmaya devam edeceğiz

@bencekomigim 6 месяцев назад

ben de ordan geldim sdkfsdmögs

@mer.2024yks 5 месяцев назад

Ben de chhdj

@sevvaltann Год назад

barış hocam yolladı bizi buralara

@noth3285 Год назад

o kim

@sevvaltann Год назад

@@noth3285 barış hoca biyoloji

@tyt-ayt6142 Год назад

Evetttt

@doktorolucam Год назад

Dr biyoloji

@mer.2024yks 5 месяцев назад

Ayn

@sumeyye_nur_esin Год назад

BARIŞ HOCAM SAĞOLSUN BİZİ DENEYLERE ORTAK ETTİ

@nisan-m Год назад

barış hocam sağ olsun

@polkadots7792 4 месяца назад

EVET EVET!

@mezunbiri Год назад

Barış hocadan gelenler yazsın buraya yks savaşçısiyizzz

@ercan.. Год назад

Barış hocadan geldik.

@hedeftp6934 Год назад

Barış hocamdan buraya transfer olduk

@esmaartunay9558 Год назад

barış hocadan gelenler

@yavuzsolak 6 месяцев назад

We brought the greetings of our valuable teacher Barış M. Kapan

@berin2004 6 месяцев назад

barış hocam sağ olsun deneyleri görebiliyoz

@tanjat123 5 месяцев назад

Barış Hocadan gelen arkadaşım sana da selam olsun

@rascalriley Год назад

Barış Hoca'dan gelenler

@mstftrk2843 5 месяцев назад

Barış Hocam'dan geldim.❤

@blairwaldorrf Год назад

barış hocadan geldim

@afiyetolsunyks 3 месяца назад

Barıs hoca kalitesiyle buradayız

@Yks_maratoncusu_boom 4 месяца назад

@DR.BİYOLOJİ HOCAMDAN SELAMLAR

@DrSohaAli786 Год назад

How many are there after studing biotechnology 12 class

@DrSohaAli786 8 месяцев назад

@DoktorOnia nice

@k47mc60 7 месяцев назад

MSC microbio

@Toluene26 7 месяцев назад

Me too

@user-mz6hi7uh5t 7 месяцев назад

@sumitroy5204 7 месяцев назад

Nah , I'm here for college lab exam

@Ksjdjdjjf Год назад

Barış hocadan gelenlere sa

@hamitmertoguz5980 Год назад

DR Biyoloji den gelenler

@gulsumbalc3786 Год назад

Barış hocam da barış hocam

@MehmetTugrull Год назад

aşwldşlawdşşad

@fadentunc578 6 месяцев назад

Dr. Biyoloji 💐

@firemeetgasoline8502 Год назад

Barış hocadan gelenler ses verin 🤝

@againstepbystep Год назад

ben ben ben

@Shyeers Год назад

ben dee 🤘

@YKS2006 9 месяцев назад

Kazandınız mıı

@bencekomigim 6 месяцев назад

asla cevap vermezler... @@YKS2006

@AslgulSert-ii8tx 9 месяцев назад

Barış hocam bir tane 😊😊

@YKS2006 9 месяцев назад

Dr. Biyoloji Dı dım tı tıs 🥁

@elenielenaki6673 5 лет назад

I just realized why I was doing this in lab because the instructor did not explain a thing! Thanks soo much! God bless 🙏🏻

@xxTAARGUS 4 года назад

Thankfully my instructor is a boss and covered all of this and used this to show the process, hope you get a better teacher next semester.

@onyebuchiblog 3 года назад

I'm interestingly interested in this

@yksascisi 3 месяца назад

Barış hocam 🤟🤟

@esra.793 4 месяца назад

Barış Hocamda Barış Hocam

@caganyavuzer 6 месяцев назад

Barış hocam gönderdi O7

@busra9257 2 месяца назад

Came from "DR. BİYOLOJİ" chanel 🤙

@hakanyigit8901 5 месяцев назад

Barış hocamda barış hocammmmm

@poetrylover5561 3 года назад

Excellent description on GEL ELECTROPHORESIS...MANY THANKS FOR UPLOADING THIS LECTURE ❤️

@LeylaKonyal 2 месяца назад

I'm coming from Barış hoca youtube chanel❤

@walidnadirulahnaf3978 3 месяца назад

1. siapkan sub sel mini 2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif 3. tempatkan egl agarosa ke ruang gel 4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm 5. tempatkan sampel sesuai urutan yang benar 6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang 7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah) 8. sambungkan elektrode ke catu daya 9. nyalakan catu daya 10. atur tegangan konstan sebesar 100V 11. atur timer menjadi 60s 12. amati perubahan yang terjadi

@eylulvehayatindakiguzellikler 2 месяца назад

barış hocamdan geldikkk

@roseofheaven_ 4 месяца назад

Dr Biyoloji🫡

@nredirul5069 Месяц назад

Bütün barış hoca yorumlarını beğendim👍

@Arpnasingh20 3 года назад

Amazing...to see the DNA fragments...1. Smaller the fragment size ,the farther it moves. 2.Agarose is natural polymer extracted from Sea weeds. 3.Separated DNA fragments can be visualised after staining with ethidium bromide.

@aleena8065 2 года назад

NCERT! 😂👌

@Mego1031 Год назад

Who the hell still uses ethidium bromide!??!? SMH...SYBR Safe has been around for like a couple of decades now...there's absolutely no need to keep using the HIGHLY carcinogenic EtBr...wow...

@bluegenes2273 4 года назад

1:56 oh, that is so damn satisfying.

@sam_khan_1995 4 года назад

When he pippet sucked the entire sample I said fukkkk so perfect

@shayisbored 3 года назад

How nostalgic. I did this as a practical exam when I was in college and completely ruined the agarose gel 😂😭😭. My prof got really mad.

@walterwhite4699 3 года назад

Damn can't wait till I go to college...

@timecode37 3 месяца назад

What happened exactly? How does one ruin the agarose?

@Sexettin Год назад

Barış Hoca sayesinde geldik

@onyebuchiblog 3 года назад

Lovely, I'm interestingly interested in this. I just finished running the three practicals now, on center for molecular bioscience and biotechnology lab in SCH

@potatoboi9298 Год назад

Hi can i ask you some questions maybe in the future for academic purposes only. thank you. is it okay if i can contact you through email?

@arresteddevelopment2158 4 года назад

So exciting, this!

@sondos8928 4 года назад

Watching it during quarantine 😭😭😝

@buba_Dukz 3 года назад

haha

@sondos8928 3 года назад

Omg i passed that semester and still corona didn’t ended

@nutallergy420 3 года назад

@@sondos8928 hahahaha I’m doing it for mine right now

@walterwhite4699 3 года назад

@@sondos8928 "ended"

@irishtaco9056 3 года назад

im sorry quarantine was a year ago-? holy shit-

@jasminanil5411 8 лет назад

very informative . thanks a lot.

@HayDayEveryday 3 года назад

Thanks

@punamchand9461 6 месяцев назад

Nomesh sir PW show this in class 😎😎 rewatching this video after class ❤❤

@papoutsothiki 3 года назад

Please DO NOT stick your pipette tip in the well while loading,you might puncture it, not to mention the sample might hit the well and spurt outside the well.pour enough buffer so that your tip is immersed but is still right above the well and release sample slowly. The loading buffer in your sample, in this case the blue stuff, contains glycerol which is heavy and 'leads' your DNA safely in the well.

@whatevervlogs9663 11 месяцев назад

I do what I want

@papoutsothiki 11 месяцев назад

@@whatevervlogs9663 you can choose to learn or not. your call mate 🤷

@srividhyanarayanan7337 8 месяцев назад

Actually I'm curious to know how to load the sample.if I simply put the sample in the well doesn't it get mixed with buffer.or u directly put the sample inside the agarose??

@papoutsothiki 8 месяцев назад

@@srividhyanarayanan7337 your sample should already have buffer in it because you use the Loading Buffer LB I use a 1 microliter of 6x loading buffer (trisborate a couple of dyes and glycerol) and 5 microliters sample therefore your LB is diluted to 1x. If you are using 1x TBE or TAE the liquids in theory are close isotonically even though the actual samples vary. do not worry. the glycerol in your LB is heavy enough it will pull the sample into your well because gravity. Just practise a bit - put the tip above the well IN THE BUFFER and slowly release sample (no bubbles) you will see your sample-dye sink to the bottom of well. Maniatis protocols explain this nicely. Let me know if you need a recipe for LB .

@k47mc60 7 месяцев назад

@@papoutsothiki I don't think that's a thing, the pipette is supposed to puncture through the gel to allow the plasmid dna/ genomic dna to enter the electrophoresis casette

@arresteddevelopment2158 5 лет назад

Thank you for this

@yessycueva2600 9 лет назад

I love it =) It's so interesting and beautiful at the same time =)

@MrHeatAz 4 года назад

So are you :p

@HemantSharma-ro1dk Год назад

This is very nailed and good knowledge thanks 🙂

@MrRisha100 3 года назад

Thank you.. Well explained

@katarina6587 7 лет назад

thanks my dude

@mehmethanyildirim1353 4 года назад

Thank you so much. I learned lessons but i never applied and i am still scared 😅

@anjalichaudhary5985 5 лет назад

osmmm video! plzz upload more more dis kind of video so that it is to understand

@charliesun1304 5 лет назад

Very cool video, well done!

@Jemiller2012 7 лет назад

very well done video

@meghanaakshaya1915 3 года назад

Very good information tq it is useful of my lesson .

@harkamanghag6877 9 лет назад

Very informative.

@hemantanayak3997 7 лет назад

excellent videos

@HussainAli-xf6bk 7 лет назад

very Informative

@sudiptachanda3486 Год назад

What should we do if there more bubble in red electrode? Please make a video on sample preparation also

@abigailtsado8720 3 года назад

Very understanding , thank you

@pisser98 8 лет назад

instead of pushing the pipette all the way through when loading the wells, you could release pressure to suck the bubble back in. that way you avoid marked sample spilling out of the wells - not that it makes a huge difference, i just like to keep the buffer clean.

@Ronnicus 7 лет назад

Or you could not push to the second stop

@HayDayEveryday 3 года назад

@@Ronnicus lol

@KarthiKeyan-kq9yu 6 лет назад

really good

@mehulmakvana8583 4 года назад

That's my favourite experiment

@sciencequizzes6641 3 года назад

Thank you so much!

@mattmeza6825 Год назад

This was awesome

@aycelllaaa Месяц назад

Barış hocam yollarsahoop buradayız

@anamikapushkar222 10 лет назад

thanks .. its good to learn the exact technique.

@medicinestudent8349 Год назад

Thank you!

@pabodhaweerasinghe9331 7 лет назад

Thanks

@boyinalabcoatboyinalabcoat393 3 года назад

for the blanck I use the leader + load dye. for samples of dna you need load dye + dna + enzime + enzime buffer, I am missing something?

@NIPUNICHATHUNIKA 6 лет назад

Thanks. It’s very clear

@hemo2458 Год назад

fantastic

@sevvalesi28 Месяц назад

barış hocadan geldikk

@qa14tusharnaiknaware25 3 года назад

Very Interesting 👍🙏

@Aevan248 Год назад

that was great thank you

@pharmasolutionpakistan7668 2 года назад

Good effort

@saradarvishvand5653 6 лет назад

very very good

@nagatsatti8426 2 года назад

Excellent thank you too much

@i.kamalesh1930 2 года назад

For the bands to be visible…. No need for ethidium bromide and uv light exposure?

@faizaquddus2848 3 года назад

A Clear video..

@Icecube88 7 лет назад

i messed this up in lab. wasted 3 weeks preparing my cheek cells to see if i was a taster or not. smh.

@d.playerallrounder2407 2 года назад

🙏thank you 🙏

@iclalcim Год назад

Harika bi sey

@unknownpitch Год назад

Thank sir, I got it❤❤❤

@adanqureshi2425 2 года назад

What do we need the buffer for ?

@Ripjaws3199 2 года назад

When we did it we couldn't really find the wells and struggled for almost an hour and a half to find the wells.

@raveeshasathsarani9182 8 лет назад

It's very important.thanks.

@HindustanKarakshak 6 месяцев назад

Nomesh sir jindabad

@youssoufoumouri8787 7 лет назад

gooood informations for less learn some choose and thanksyou

@kaifanwar6085 6 лет назад

great!!

@salazardelmar7235 Год назад

Why do we see more bubbles at the black electrode ?

@aditikaple6958 10 лет назад

thanks helpful

@cemreozturk3137 6 месяцев назад

@raviph80 7 лет назад

its nice

@rameshdubey832 6 месяцев назад

Thanks ❤

@dannieel1327 5 лет назад

Tysm sir

@maryamsalih3801 Год назад

how I can label the product of gel electrophoresis by which program if my gel image was taken by JPA please kindly for you recommend me thanks

@dlhawari5086 2 года назад

Hello, any distributor of this instrumentation and consumable in Indonesia? Thank you

@amritas2400 5 лет назад

Thank you! This helped a lot! But hey, where's ethidium bromide and UV rays? And bright orange bands of DNA?

@Gayu_raje 5 лет назад

Yes.. Atlast we visualize clear bands by uv rays .. They didn't mentioned it