I want to personally thank you for the quality of these videos and both their general, universal applicability and their specificity. My labs on genotypic identification were cut short by COVID-19 and we cannot do 'wet labs' for this topic anymore. I was disappointed by the dearth of virtual labs on the entire sequence of how ID by DNA occurs (from start to finish), or that they were heavily watered down or not specific enough for sophomore/junior level microbiology courses I teach. But I feel confident I can show some of your videos and give them their sequencing data, and they will understand the procedures and importance. Please do more educational videos!
What I don't like about using PCR for the Covid is they use this in reverse to get RNA from DNA after which they still have to guess at what they see Because Exosomes which are abundant in humans also give off RNA and are a part of our immune system As far as virus though they cannot be sure what it is they are testing for afterwards which something this small afterwards they are simply guessing after sequencing for a virus if they believe they find one but again are they?
Enzymes are difficult/expensive to produce, and most would become denatured at the temperatures used in the thermal cycler. Heating it to 94 is cheap, easy, and dependable!
wait I'm confused, if the optimal working temperature of polymerase is 72 degrees (4:41), then why would it denature at "these high temperatures" (4:56)?
72 degrees is the optimal working temperature for Taq DNA polymerase, the one found in hot springs. The polymerases that would denature at 72 degrees are the ones from humans or typical bacteria. She mentions that a few seconds before the 4:56 mark
I'm watching this for a microbiology class wondering if in fact some people are just better able to understand this stuff than others. I'm not able to retain this information because it just doesn't make any sense to me. It's discouraging and it's by no means the fault of the person creating the video. The video was just fine. I get really down on myself about this stuff though. Things would be a lot easier if I could actually understand it better.
Great video. Im at novice in behavioural genetics, and still struggle to understand how the sequencing is helpful? Can you really from having just a few nucleotides to start with, work out the rest of the whole sequence through this PCR process? If I give you A, C, A, T, can you with the help of these enzymes figure out the rest?
So ...... as I understand it. In case that no DNA piece matches the primer no multiplication will happen? Does that mean that fx. PCR-test fpr covid-19 does not produce false positives?
In a well-designed PCR, the primers will be specific to the sequence that is being amplified. This makes false positives from a PCR test very unlikely.
PCR is a method of amplifying and replicating DNA in vitro! It's similar to the process that happens in living cells, but reproduced in a controlled lab setting.
Wow, when physicists talk about forces, momentum, acceleration etc they always drop Newton's name somewhere along during their presentation. Engineers drop names like Faraday, Watt, Babbage etc. Not once throughout this presentation did the learned doctor mention Kary Mullis, whose body, at the time of her upload (Feb 5, 2020), was barely decomposed. The poor man was screwed over by Cetus, so often got banned from speaking to make some money, and now his lifetime's work is being talked about without even a mention of his name as its inventor. That's quite disrespectful, in my opinion.
Mullis did indeed invent it, and a great deal of work would not have been possible without his work. The trouble is that Mullis later in his life started taking some decidedly non-scientific views. He's entitled to whatever he wants to think of course, but it did mean that he fell out of favor in many scientific circles.
pcr is not a test, its a production (multiplication) process, it does not tell you anything about the nature of the entity that you are making copies of.
@@jcezary scuse me... all the government use pcr to tell people that are infected or not these days. is that possible that you use a pcr to find a covid 19 genetic target but the technnique amplify another target ? that's my question. are the tests precise ?
@@jcezary scuse me ... how does it work ? if i know the target of covid... and i develop a primer for covid 19, can the primer attach to the normal influenza virus and amply another dna sample ?.... the question is are the PRIMER specific only for covid ? or there's a risk that i've ifluenza and i test positive for covid ?
