Instruct-ERIC and ARBRE-MOBIEU Workshop, February 2020. Analysis of protein folding by CD spectroscopy. Presented by Arthur Sedivy of the The Vienna BioCenter, Vienna, Austria.
Dear Cardon, we are glad that you found the video helpful. Please can you send your email address to support@instruct-eric.eu with your question, and I will pass it on to the presenter. Many thanks, Instruct-ERIC
We have a protein sample, which is being used at different temperatures and pH and we are seeing vertical (i.e., molar ellipticity magnitude) shifts in the CD spectrum. It cannot be due to concentration because it is all the exact same sample. We did a 222nm/208 and for the most part there is linearity across temperatures at pH 7. However, at lower pH the 222nm/208nm plots show kinks at the higher temps, but there is no "directionality"/correlation between the magnitude of the vertical shift and temperature (or pH). The shifts seem quite random. Any advice? Suggestions? Should we do WSD to be able to say "There is no significant change in secondary structure?"