Circular Dichroism Spectroscopy for Protein Structural Analysis

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Circular Dichroism Spectroscopy is a useful technique for characterization of secondary structure of proteins. Additionally, it can be used to monitor protein structural stability, cofactor binding sites in proteins, and to study protein folding. I discuss the theory and applications of CD Spectroscopy in this video.

Опубликовано:

28 апр 2021

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Комментарии : 34

@debesray Год назад

Describing using animation was fantastic for a clear understanding, which we miss in the papers or articles. Thank you.

@SpartanTutorials Год назад

Most welcome, Debes. Glad to know you found it helpful.

@viktorija400 Год назад

This really helped me understand this study. I was reading it before I watched the video and I was very confused, and now that I watched the video it is very clear how it all works. Thank You !!

@SpartanTutorials Год назад

My pleasure Viktorija. Glad to know you found it helpful.

@mthakur1987 2 года назад

nice information gathered to talk on CD. Keep it up

@ryoichitaguchi232 Год назад

Thank you so much for this!

@user-zb5zk2yp2k 7 месяцев назад

found this video very helpful, since there are no studies on CD in my native language and reading an english article provided by my prof (chemistry student here) didn’t make sense to me. thanks a lot! hopefully i’m gonna pass my test thanks to your video

@SpartanTutorials 7 месяцев назад

Glad to know it helped; Best wishes!

@khushalithaker9507 Год назад

Helped a lot... Thank you sooooooo much sir!

@SpartanTutorials Год назад

Most Welcome Khushali!

@maksimnovikov4238 Год назад

Thanks a tonn!! Are there any other CD resourses you recomend? Especially for cofactor binding?

@SpartanTutorials Год назад

Thank you Maksim. Here are a few resources for studying cofactor binding using CD: www.researchgate.net/publication/7304807_Probing_Protein_Binding_Sites_by_Circular_Dichroism_Spectroscopy www.sciencedirect.com/science/article/pii/S0005273613002083 ctrstbio.org.uic.edu/manuals/kelly.pdf

@kajalguleria7615 3 года назад

Thank u so much sir..this was best explaination

@SpartanTutorials 3 года назад

Most welcome, Kajal. Glad you found it helpful.

@matildeermanni7790 6 месяцев назад

thank you

@SpartanTutorials 5 месяцев назад

Glad you found it helpful.

@hanumanthpanuganti5185 2 года назад

Thank you sir

@SpartanTutorials 2 года назад

Most welcome Hanumanth.

@atul281979 5 месяцев назад

great job very nice

@SpartanTutorials 5 месяцев назад

Thank you!

@shraddhabose3330 Месяц назад

very nice! well explained. which buffer do we need for CD run? I think 20-50uL of 0.2-0.5mg/mL concentrated protein is required right?

@SpartanTutorials Месяц назад

Thanks! You'd need at least 150 uL for a 1 mm path length cuvette. Regarding protein concentration, 0.2-0.5 mg/mL is a good start. However, it may vary depending on the secondary structure content. You can start with a concentrated protein solution and dilute accordingly. Check out the following links for more details: 1. www.ncbi.nlm.nih.gov/pmc/articles/PMC2728378/ 2. cmi.hms.harvard.edu/files/cmi/files/cmi_cd_getting_started.pdf 3. structbio.vanderbilt.edu/wetlab/cd.sample.prep.php

@shraddhabose3330 Месяц назад

@@SpartanTutorials Thanks a lot! Truly appreciate your help.

@ABR_83 2 года назад

Great video. How do I normalise my data by the number of peptide bonds?

@SpartanTutorials 2 года назад

Thanks Andrea. I'm not quite sure, but here is a resource that may be helpful: Greenfield, N. J. (2004). Analysis of Circular Dichroism Data. Numerical Computer Methods, Part D, 282-317. doi:10.1016/s0076-6879(04)83012-x

@samarthkumar5198 2 года назад

Sir, How can we study conformational changes in a protein structure?(Except the conventional techniques we have)

@SpartanTutorials 2 года назад

Well, we can study them using techniques like FRET and doing crystallography of different conformations. Additionally, there are more specialized techniques. Take a look here - en.wikipedia.org/wiki/Conformational_change

@manishachauhan7046 2 года назад

👏

@SpartanTutorials 2 года назад

Thanks Manisha.

@umarhasnain7369 10 месяцев назад

15:31, why is this dotted line inactive?? How will I understand that??

@SpartanTutorials 10 месяцев назад

The dotted line is not inactive. It is the CD spectrum of an inactive protein. The protein mutant used in the analysis is inactive and has lost its activity. This loss of activity is confirmed by other assays.