Amazingly Explained but I have to watch 3/4 times to grasp all the ideas, It's actually deep, Would you please make a video on bacterial engineering using arabinose operon?
The type of content that higher studies require, amazing explanation. Can you please see if you can post a detailed explanation on cell cycle regulation.
Great content! If I could (somewhat rudely) make a request it would be for enhancer activity and cohesin looping explanations. Anyway thank you for putting in all of this effort, it's appreciated.
Absolutely! I have been planning a series on genome organization (3D and otherwise) with TADs, compartments, loops, and other interesting things. Just need to find some time to actually produce stuff :)
Very nice explanation sir. I have a small doubt Arabinose when present needs to bind with the dimer of ara C for proceeding the expression of the structural genes. Now since ara c dimer is needed for arabinose to bind, so why does it needs to get regulated by blockage of the promoter of ara c since ara C protein is a necessity.. 😬😬
Hello. I don't think I said that araC promoter is blocked. Refer to 18:10 in the video. It is simply that unlike araBAD, the araC is not upregulated when arabinose is present. This is simply because, you don't need a lot of araC protein to overexpress araBAD - basal levels are sufficient. Note that the araC is somewhat repressed when arabinose is not present but when arabinose is present it is not repressed, rather it returns to a basal expression.
Can u explain how viral promoters or other plants promoters expressed in other organisms totally As we know, activation of promoters of different genes required different sigma factors?
araC gene i.e. CDS (not drawn for most of the video but see 9:42) is downstream of the operator. The O2 does not interrupt the CDS; the operator is between araC promoter and the araC CDS.
