AS Biology - Enzymes topic. Description of how to use vmax to calculate Km (the substrate concentration at which 1/2 Vmax is reached) and what it tells us in terms of the affinity of an enzyme for its substrate.
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Vmax is steady because when enough substrate is present, enzyme becomes fully saturated (all of its active sites are bound by substrate) and thus it is operating at its highest rate - it cannot bind more substrates so the rate cannot increase. The rate does not change anymore no matter how much more substrate you add
