Aseptic Techniques: Cell Culture Basics 

Thank you for the suggestion! We'll let our lab scientists know to think of some good icebreakers for future experiments.

Lmfao

I have a question please Why we should not set down the inoculations in any surface? Although we've already sterilized the surface.

True🤣🤣🤣

@@julianarose9399 because immediately after wiping clean there's a chance for airborne contaminates to settle on surfaces.

Oftentimes as you pour, no matter how quickly and deliberately, a tiny amount of media can accumulate in the lipped area of the flask and possibly drip down. This creates a pathway into the flask for contamination, and also can contaminate the flask lid when you screw it on. Thus, it is not considered proper aseptic technique to dump liquid from flasks. The only thing we dump liquid directly out of are deep Petri dishes, which can easily have any media on the lip of the plate wiped off with 70% ethanol.

@@paigejohnson3291 Thank you. A good reason indeed.

Hello, Thanks for your question. Can you please reach out to our technical support team at thermofisher.com/askaquestion. They would be the best team to assist. Thank you!

Because using same pipette for different samples will mix the two samples

Are you asking for a friend?

@@thermofisher no, i dont think anybody wants to passout while wearing a bio suit in a lab or throw up

Can you please tell the source of this information?

typically the packaging itself of the ethanol will state this. I have seen some bottles where it says it should even be left for 5mins.

@@pratika.ambekar8778 read. Alcolhol rub

@@LaurinSchaller some microbs 5 Min lther 2 min

Snap.recent graduate looking for a job. Miss being in a lab so much.

@@paulreynolds8632 Did you find a job?

No, face lid down, not up. The surface area is already sterilised with alcohol so there should not be contaminated. Whereas if you face the lid up, you are more likely to accidentally touched the inside of the lid with your fingers or hands or even other items. Also, the inside of the lid is more exposed to the air, which risk further contamination

"Lids up" is more of a chemistry practice. In biology, having the lid up exposes the inside of it to the downward flow of air, and passing one's hands over the exposed surface can blow contaminants into the lid, and subsequently into the main bottle or flask. I can understand the concern about having the rim touching the BSC surface, but ultimately it is the lesser of two evils. "Lids down" has served me very well for 2 decades as a cell culture professional.

Question: Why we just do not empty the flask by turning it upside down in place of media aspiration ? What could possibly go wrong ?

What is the composition of the liquid used for discharge cells that are not used anymore in the process ?

The woman is working within a biological safety cabinet, which is an enclosed workspace. Class II BSCs are recommended for use in cell culture. If you look close enough, you can see the clear sash is pulled down in the front and covering the workers face and body allowing only her hands and arms to work inside the cabinet. To understand how the BSC works and protects the cell culture from the outside environment, the customer can reference this video, also by Thermo Fisher Scientific: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-oIuWQqzw324.html

research does not have to follow GLP and especially not GMP