Basics of lab centrifuges - rpm vs. rcf, swinging bucket vs. fixed angle, ultra vs. "normal", etc.

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Lab centrifuging can be kinda confusing. So here’s a guide to the basics of what you should be using!
And here are some nice resources
A primer from Beckman: A CENTRIFUGE PRIMER - Beckman Coulter
media.beckman....
For calculations and conversions: www.beckman.co...
I will work on some text and that practical post
But in the meantime, as always, hope this helps!
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbioc...

Опубликовано:

8 сен 2024

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Комментарии : 15

@merajj Месяц назад

great work as always

@funny11744 Год назад

Great work.Many explanation, a real teacher.

@thebumblingbiochemist Год назад

Thanks so much!

@kevintotimeh6648 Год назад

Excellent video as always, it makes sense why Newtonian forces are applied to the centrifuges' rate of motion. Also, interesting units, I never heard of rcf.

@thebumblingbiochemist Год назад

Thanks!

@CheMisTcLuB 6 месяцев назад

thanks a lot, you have no idea how much you help with this explanation

@thebumblingbiochemist 6 месяцев назад

Glad to hear that!

@IlmiMulhid Год назад

Thanks 🙏

@funny11744 Год назад

I have a 10 ml centrifuge with fix angle and a minimum radius and a maximum radius . I do not find the radius in the centrifuge manual but it is written RCF =1150 g for 3200 rot /min. What would be the (average) radius for calculation of RCF ? Is it 10 cm ? Could the radius be artificial increased by decreasing the volume in the tube from 10 ml to 3 ml for example ?

@thebumblingbiochemist Год назад

I don't know sorry but changing the volume won't change the radius

@funny11744 Год назад

@@thebumblingbiochemist IT depends on the radius .If it is the maximum radius - the RCF does not change but If it is about an average radius ( for fixed angle centrifuge only) between the minimum and maximum, then RCF is changing once with decreasing the minimum radius ( meaning decreasing of the level of the liquid in the tube).

@thebumblingbiochemist Год назад

Gotcha. Sorry I misunderstood your question

@funny11744 Год назад

Could the Amicon 100 be used with fixed angle centrifuge ?

@thebumblingbiochemist Год назад

Yes - but you may need to adjust the speed and volume - the product manual will have more information.

@funny11744 Год назад

@@thebumblingbiochemist thank you.