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Bioprinting 101: How to make Microfluidic Chips 

Bioprint Academy (previously SE3D)
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25 окт 2024

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Комментарии : 47   
@rock3tcatU233
@rock3tcatU233 3 года назад
Is it advisable to use a vacuum pump in order to quickly remove the air bubbles in the PDMS?
@erwinz5926
@erwinz5926 2 года назад
looks like they used one. propably it is. i thought the very same watching this.
@khaledfreij6586
@khaledfreij6586 5 лет назад
Can i have more info about the dimensions of the channels?
@danielayala646
@danielayala646 5 лет назад
cool! how many more times can the printed design be casted? or doesit disinttegrate aftyter first cast?
@SE3Dedu
@SE3Dedu 5 лет назад
It's one print per cast. Once you cast, it solidifies over time. Then you can wash off the channels.
@RuyRuiz
@RuyRuiz Год назад
Love it, omg you really make sounds so easy. New Subscriber.
@weitingLin66
@weitingLin66 5 лет назад
nice introduction! make it pretty clear
@mayasarilim3611
@mayasarilim3611 5 лет назад
Thank you for the feedback. Glad this was useful.
@carlosvelazquez6440
@carlosvelazquez6440 3 года назад
Man, I'm just trying to figure out how colors mix to produce different colors, like is it linked to turbulance and if so is it a chemical reaction, or an optical illusion.
@theglobalwarming6081
@theglobalwarming6081 3 года назад
The color is based on the pH of the fluid. I think they used one universal pH indicator for both samples. So, when they change color, they change due to a chemical reaction.
@kryogenica4759
@kryogenica4759 5 лет назад
Are the channels exposed to the open air on the bottom face?
@SE3Dedu
@SE3Dedu 5 лет назад
Yes we use tape to cover the channels so you have a seal to flow fluids through. Otherwise you can use a glass slide.
@kryogenica4759
@kryogenica4759 5 лет назад
@@SE3Dedu Thank you so much for the info! Will see if these can be implemented in a classroom.
@milascatadventures1011
@milascatadventures1011 5 лет назад
@@kryogenica4759 If you go to portal.se3d.com and sign up for a free account, you can find our print files and instructions there, under Additional Resources, Bioprinting Project Kits.
@a.n.9227
@a.n.9227 4 года назад
Hi can you send me a quotation of prices of the reagents you used? PDMS, Fpluronic, ... or even redirect me to someone who could do so. thanks a lot
@maxfreudenstein6892
@maxfreudenstein6892 3 года назад
The music gives me hope that som day our peple shall be free again
@visionbios6736
@visionbios6736 4 года назад
Any recommendations on how to attach the PDMS device onto a glass slide ? Any help is much appreciated
@mayasarilim3611
@mayasarilim3611 4 года назад
Just make sure both surfaces are clean and dry before attaching the glass slide onto the PDMS cast. Good luck!
@Amyhb69
@Amyhb69 3 года назад
Plasma
@sharadambardar9252
@sharadambardar9252 2 года назад
oxygen plasma treatment
@bhatt6873
@bhatt6873 2 года назад
You have to go for O2 plasma etching of the glass slide after which you bond it with the PDMS
@nebuchadnezzar47
@nebuchadnezzar47 4 года назад
why dont they just completly 3D print the end product in plastic? with channels built in?
@TheMrJuoji
@TheMrJuoji 4 года назад
depending on the kind, but fdm is out because of the porosity , i don't know for resin printing but i guess you'd need something chemically inert , as for slm/sls, it's probably feasible yet would be an expensive piece of equipment
@ignacioaguirrenoguez6218
@ignacioaguirrenoguez6218 Год назад
They are doing that nowadays
@amitasthana45
@amitasthana45 Год назад
can you please send us the dimension or STL file to me
@aslcatalyst416
@aslcatalyst416 5 лет назад
Hi is the CAD for this part available for download?
@SE3Dedu
@SE3Dedu 5 лет назад
Pls visit portal.se3d.com and sign up for free to access files, under Additional Resources, you will find it under Bioprinting Project Files
@baltakatei
@baltakatei 2 года назад
@@SE3Dedu lol, it's dead.
@zakyrayadhirh
@zakyrayadhirh 4 года назад
can I get a paper from your research?
@PrasannaKumar-zx7gr
@PrasannaKumar-zx7gr 5 лет назад
Really interesting
@lintong8703
@lintong8703 Год назад
Pluronic 127 for creating the mold / channels
@Muradsahar
@Muradsahar 3 года назад
Amazing!
@Farhan-co9he
@Farhan-co9he 2 года назад
impressive
@vivianaclaveria9123
@vivianaclaveria9123 4 года назад
This is not microfluidics. This is millifluidics. Nice video and technique anyway.
@SE3Dedu
@SE3Dedu 4 года назад
Yes you are absolutely correct. More for demo and educational purposes.
@pubgamedz
@pubgamedz 2 года назад
Whey i Can't download! 🛸
@PrasannaKumar-zx7gr
@PrasannaKumar-zx7gr 5 лет назад
👍
@Karpe_Deem
@Karpe_Deem 3 года назад
3:44 minutes for a fraction of a second of demonstration 😢
@erwinz5926
@erwinz5926 2 года назад
so- the result is green yellow!!!11! *scnr.* : )))
@maracachucho8701
@maracachucho8701 4 года назад
Not very "micro", though!
@miron__
@miron__ 5 лет назад
fix your audio
@wirehyperspace
@wirehyperspace 7 месяцев назад
Lego land
@georgen9755
@georgen9755 Год назад
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@georgen9755
@georgen9755 Год назад
bottom line .......................................... via ......whose e-mail ........... primary account ...............mailer ............... I don't need your washing machines .......... ... I don't use owners washing machine to wash my clothes ........
@mdgar6516
@mdgar6516 4 года назад
quit the music, it is irritating
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