As always, the steps of Western blotting depend on your lab and protocol. Here, we look at the general theory of the Western blot and what it is used for in the lab.
Quite helpful. I was confused on the part of screening but i think this video puts it together nicely. So, it's a conjugated enzyme that finally binds to the secondary antibody. On binding, the enzyme converts the colourless substrate attached to it into a colourful insoluble product that sinks down to the nitrocellulose membrane emiting colour. Got it! 💪
