Biotechniques | Principles of SDS-PAGE (Protein Separation) 

i tumbs up for you can directly speak out the professional words!

First off: amazing explanation - thank you so much! Now for my question: for the SDS-PAGE after you run electrophoresis, at 11:34 why are the four different lanes showing less and less proteins as you move across? are you performing some sort of washing step? If that is the case, how is it done? And furthermore, if by lane 4 you can see your protein of interest, why do a western blot?

PERFECT

Very helpful

This is a helpfull vedio

super helpful, but does the sodium from sds do anything? like where does it go?