Welcome to Biology with animations! In this video, we explore the fascinating world of ELISA (Enzyme-Linked Immunosorbent Assay). Learn about the crucial steps involved in competitive ELISA, from plate coating to protein detection and quantification. Discover the significance of reagents like Tetramethylbenzidine (TMB), enzyme labels such as Horseradish Peroxidase (HRP).
🔬 Topics Covered:
Competitive ELISA Technique
Use of Conjugated Proteins
Washing and Blocking Steps
Detection with Chromogenic Substrates
Absorbance Measurements with a Spectrometer
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27 сен 2024
