thank you for the video , i usually never autoclave the media , specially not the selective. i test a small quantity for contamination and if it is okay i do not autoclave the , i just heat it for 10 muntes after the agar has totally melted and it does the job.
For sterility assurance, we do recommend autoclaving the media. If you have any questions or want to dive deeper into the "why" of it, feel free to reach out to our Technical Services team at TechnicalServices@HardyDiagnostics.com, they're an amazing team who'd be happy to explain more!
Thank you for your question. The hose is actually a temperature probe. As the video explains the probe can be placed directly into the media (which is in the Erlenmeyer flask aka “Erlen”). As the directions state: “Place the flask of boiling media into an autoclave to sterilize. If your autoclave is equipped with a temperature probe, the temperature probe can be placed directly into the media and the cycle parameters will be based on the readings of the probe.”
