Counting cells using a hemocytometer video protocol 

6:04 If my calculations are correct, if you have a viable cell count of 200,00 cells/mL in a total volume of 20 mL, then that should be 4,000,000 cells. If you then want to to seed 10,000 cells into the new flask (0.0025 or 0.25% of the total cells), you should transfer 0.05 mL (50 mcL) of the original sample into the new flask.

Wow, counting cell with inverter microscop. Please mention the type of hemocyometer ?

6.07/6:05: Formula= Desired number of cells to seed/Total number of cells=Volume to transfer/Volume of the cell suspension 10000/4000000=volume to transfer/20mL 10000/4000000*20=0.05mL which is equal to 50 microliter Further Total cells=4000000 and total volume is 20000ul so 1ul contains= 4000000/20000=200 which multiply by 50= 50*200=10000 cells

For anybody stressing over the calculations at the end, these are wrong.

ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-GCohewzhD3k.html, For the 2nd fomular, are you sure you want to use count1 to divide by 4, not count5 divided by 4?