Counting yeast cells with a hemocytometer
We will show you how to use a hemocytometer to count cells and share some tricks so that you can do it right. Various types of Hemocytometers exist. The most popular ones are Neubauer or Thoma Chambers (Neubauer improved chambers and Neubauer chambers).
For the exercise you will need the microscope, hemocytometer, its cover slip, ethanol, your sample and the pipette with tips.
Video includes the following parts:
PREPARING THE HEMOCYTOMETER
LOADING THE SAMPLE
THE COUNTING GRID & GENERAL COUNTING RULES
EXTRA RULES FOR YEAST COUNTING (including rules for counting budding cells and stained cells)
CALCULATING THE CONCENTRATION (including the basic formula to determine concentration)
GENERAL TIPS
CLEANING THE HEMOCYTOMETER
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Zac Roger Yeates
Kamilija Tekle Cizaite
3 окт 2024