That was too good; however, I've gotta watch this video over and over again to understand how all these information brings together :D Thank you for making up my mind to study smarter
The codons CGT, CGA, CGC and CGG, all code for arginine; spontaneous deamination of 5-methylcytosine in these codons would result in one of these codons: TGT, TGC ( cysteine), TGA (STOP codon) or TGG pryptofan, instead of arginine. Interesting reading here: www.ncbi.nlm.nih.gov/pubmed/28523359
The probability of getting CG should be 12.5 no? I understand that getting exactly GC instead of CG is 6.25, but it shouldn't matter which one comes first C or G. I don't understand why you don't put that in the video, it would help understanding the low occurence even better. Am I correct? Just trying to understand... otherwise amazing video and really helped me with my paper.
I'm thankful for this video. I am interested in understanding why many cancers are caused by errors in the methylated X chromosome, rather than in the active, less methylated X chromossome, in a given female somatic cell. One of my hypothesis to try to explain it, would be the lack of access to polimerases, so proofreading wouldn't correct errors. I have also thought that, since the breakpoint regions that I read about* are indeed palindromic, as are segments of bacteriophages that are target by bacterial restriction enzymes, perhaps when the nuclear envelope of a cell is dismantled during mitosis, palindromic regions of cell DNA could lack CH3 groups and could be attacked by a restriction-like enzyme, in a similar fashion like bacterial restriction enzymes attack palindromic segments of bacteriophages. (* such breakpoints are implicated in leukemias).
Good morning, excuseme, one question please, the methilation of island GC with SAM integration diet 400 mg/today, or other methylating agents such as choline, pratically prevents the formation of cancer cells or increases them ? Thank you very much
Very clear explanation and I love the illustrative animation! Only the swirling colorful backgrounds I found a little bit irritating/distracting sometimes :) otherwise, amazing video!
What does percent GC mean? = guanine-cytosine content In molecular biology and genetics, GC-content (or guanine-cytosine content) is the percentage of nitrogenous bases in a DNA or RNA molecule that are either guanine (G) or cytosine (C).
Beautiful explanation.. Finally able to connect the dots.. Can you explain why is methlylation more frequent on adenine and cytosine than guanine and thymine?
Thanks for the video! I was just wondering why the methylation of cytosine bases, followed by the reamination to thymine doesn't lead to a decrease in the percentage of all cytosines in the genome (AC, TC, CC, GC, CA, CT? What is so special about the addition of the guanine on the 3' end of the cytosine?
I love how they always explain what are the bases that compose the DNA. What are the possibilities that you are looking for what a CpG island is without already knowing that?!
