CRISPR Cas9 Screening

The Ahmed Lab: Northwestern Neurosurgery

Подписаться 5 тыс.

Просмотров 15 тыс.

50% 1

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021
This video provides an overview of setting up and conducting a large-scale CRISPR screen in the context of Glioblastoma (GBM). Although we focus on GBM here, the concepts are applicable to a wide range of scenarios. Our ongoing CRISPR work is performed as a collaboration between the Sonabend and Ahmed Labs at Northwestern Neurosurgery, supported by NIH/NINDS.
For a higher quality version - see expl.ai/WBLAHKC
Papers and figures referenced:
www.nature.com/articles/s4146...
www.cell.com/cell-reports/pdf...
www.computescotland.com/image...
www.cell.com/cell/pdf/S0092-8...
blog.addgene.org/plasmids-101...
www.thermofisher.com/us/en/ho...
www.axolbio.com/page/transfec...
www.mirusbio.com/applications...
pharmaceuticalintelligence.co...

Наука

Опубликовано:

30 июн 2024

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 34

@ksn4911 4 года назад

I was struggling to understand a paper related to CRISPR screening and this lecture was very helpful! Thank you!

@ahmed-lab 3 года назад

Great to hear!

@dianaprincess9458 2 года назад

Me too

@ranxinzhang4446 2 года назад

Great lecture! Love how she explained everything so clearly. Thank you!

@ctrlaltdelicious6072 4 месяца назад

Wow this was the most useful video on transfection/transduction/CRISPR I have ever seen and I am so thankful for content like this. I am preparing for a job interview :)

@vickyweechee 2 года назад

Thank you! Very great explanation, you help me a lot on understanding this.

@biggjones001 7 месяцев назад

I loved this totally! And a great voice to go with the explanation. Thanks

@hinaldhaduk7506 3 года назад

very informative, easy to understand. Appreciated for uploading it.

@botan3020 Год назад

A Spectacular Video! I learned a lot! Thank you!

@selamibaglamis6007 3 года назад

Great explanation, I learned so much. Thanks a lot :)

@us_visa_apply 7 месяцев назад

Thank you so much for such informative and easy-to-understand videos. Appreciate it a lot.

@nirmalapun3945 2 года назад

Amazing. Such a clear explanation. Thank you so much.

@mengzhao7911 3 года назад

Great explanation, I learned so much.

@meryemsinemuyar6233 2 года назад

the best explanation that I saw! Thank you

@dianaprincess9458 2 года назад

Thank you after I listened many videos, I couldn’t understand, I got very clear from this video.

@gokhanakman9785 Год назад

Very helpful and great explanation of the CRISPR Cas9 Screening!

@AnhVu_8891 Год назад

Thank you so much, OMG, I really appreciate your incredible lecture. It's very clear to understand about CRISPR-Cas 9.

@anjanitubati2193 Год назад

Thank you so much !! Great lecture with lot of details explained very neatly. Many doubts were cleared.

@shawsheryl5092 6 месяцев назад

So clear! Thanks!

@naimehrafatian3946 3 месяца назад

it is the best video of the technology I saw so far

@aysekaymaz6046 4 месяца назад

Great explanation! Very helpful

@salahadlat 3 года назад

This lecture is very helpful. Thank you very much

@liuliuqiu03 3 года назад

very useful! Thank you!

@xchan6905 2 года назад

Very useful and easy to understand.

@DhamminiEkanayakeMudiyanselage Год назад

Thank you very much a wonderful video

@saintlukman 2 года назад

Very useful and simplified

@George-rq1yp Год назад

Excellent!

@damialaoalao7137 Год назад

This was really good

@mamiburgac2032 2 года назад

First of all, thank you for your effort. I have a question about the numbers of the construct. Why there are 77,000 constructs, and how to determine these numbers? Could you basically explain, please?

@EDYAJ353 2 года назад

The human genome contains approximately 20 k genes (here they say 19 k), she also says that you want to have 4 sgRNAs for each gene just in case one doesn't work. So 19 k times 4 sgRNAs per gene is approximately 77k constructs. :)