Thank you soooo much! This video is extremely helpful. Should mention that you have a nice voice and pronunciation, it's so important for a non-native speaker:)
Thank you so much. The way you explain by breaking everything down makes it so easy to understand. I hope you make more videos like this. Also, your way of articulation and slides are amazing and never bores me out.
I have been looking for a good source for gRNA design for several weeks. Only with this video, I could find the answer to almost all of my questions. Thanks a lot, Jacob. Great job. Would you please add a quick video on how to design gRNA for CRISPR/dCas9 system for gene expression regulation? Thanks
Great video man, my professor just isn't all there and no one in our course understood how to design a gRNA :/ With your Video I just get it now. Thanks for the clarity and especially the explanation how the algorithm works and how you could do it yourself. Such a great help thx again
Comprehensive details, the Best method of explanation. I am gonna subscribe and like your channel to support your generous efforts. Thanks btw for the time and effort you put into this video.
Thanks a lot Jacob... .. . Even few of my Postdoc Colleagues were not able to make me understand it like the way you did... .. . Really appreciate it a lot
Thank you. Please make more videos on techniques used in the field of Biotechnology. The way you explain procedures and processes is very easy to understand and follow. You, sir, are amazing.
Great video, thanks for taking the time to explain how it works, this is great help. I have one question, how should I proceed if I want to excise a potion of an intron and remove it completely? Thanks in advance.
Such a clear and informative video! I had a quick question. I am looking to knock out a gene cluster in bacteria. Would this procedure work in bacteria as well? Thank you very much 🙂
Hi Jacob, I am glad I found your video! It is really helpful. However, I still have one question: if you have these sticky ends between gRNA and scRNA, how do they assemble correctly?
A wonderful and informative video. I had one quick question, I'm looking into knockdown of a gene (INPP5D) in human genome but am wondering the main differences between the two options for homo sapiens provided by chopchop. If you could clarify the significance that would be very helpful. Thank you!
I found your video more than useful! Thanks a lot...and I have a question! there is a way to see in what exon is located targets sequences ? I mean, not manually checking every exon in the gene... If you work with a big gene, with lot of exons, it can be pretty annoying look for your target sequence just checking sequence along.
Thank you, the video is a great help; but what about the scaffold RNA? is it included in the plasmid in addition to Cas9 encoding gene? In this case, we do not design the plasmid, we purchase it from some company!
Hi Jacob! very helpful video. Thanks. Anyone has any idea about how to make a plasmid that induces double KO for 2 genes? Or better to say, how to make a double KO cell line.
