Dehydrogenase Activity in Extracts of Chloroplasts (the light dependent reaction of photosynthesis) 

thanks for saving my life cause our teacher had no idea how to do this and taught us it completely wrong :)

Drinking game; take a shot every time she says 'okay'

Great video! Have to do something similar and write it up as part of a biology SKE but don't have chance to see the experiment live. This is a massive help and really well explained. Bit of an abrupt ending though...

So cool! It's like a science cooking show. Regards from UF

The best video on this thank you!!

Thank you so much this video was a live saver.

Thank you so much!! This video helped me a lot. My TAs are so confused and confusing. They always do mistakes, some of which are intolerable!!! Last experiment was about cellular fractionation, and they used 8000 g by mistake instead of the written 800g in the manual as the first centrifugation. About 95% of our experiments have failed. They even failed to do Gram staining properly. Let alone their broken English and intolerance to students' questions. They almost rarely understand the questions asked anyway, and even if they understand the question they most probably give very misleading and very wrong answers. I'm so jealous to know that some students have such good professors and TAs. Good lock guys and please appreciate what you have!

Really helpful

thank you!

I cannot seem to find the Note on how to make the Isolation Medium, can anybody direct me please. I do not know what the isolation medium is made up of.

Thanks for this. We did the practical but either had no colour change or just didn’t time it and our write up is due tomorrow.

Is it okay to use another buffer which is not phosphate as long as it has ph7? Our school somehow does not have phosphate buffer

What should the concentration of the DCIPIP be?

Thanks

For the isolation medium, can the sucrose and KCl be added in liquid form?

4:09 Why does centrifuging at high speeds cause chloroplasts to sediment as pellets? I thought they were part of the supernatant since they're lighter in mass and thus less dense

what statistical test would you use to analyse the results?

I thought the first pellet would be the nucleus and that you had to centrifuge again at a lower speed to get the chloroplast.. can you explain this to me please?

where is the note on preparation of isolation medium?

I had to do this experiment in Biology 101....given the complexity of the experiment that doesn’t seem right...

where is note on preparation of loading buffer ?

The result shows that red light gives higher rate of photosynthesis than blue light. I thought blue light has shorter wavelength and higher energy and should give higher rate? Please help...

where is preparation procedure for phosphate buffer

what is the isolation medium needed for?

Is this for a new spec ?

Hi, can I ask what speed is the centrifuge set at?

Give it. A try. Should be ok. Make sure your spinach is really fresh too.

Thanks

where is the note on preparation of isolation medium?

where is the note on preparation of isolation medium?