Hello Ma'am , actually i have one problem during squashing the root tips. the stain is moving out of the cells and i can't see any mitotic phase. please give me some suggestion.
1. Add 10cm^3 of 5% yeast suspension to three boiling tubes labelled A, B, C 2. Add 10cm^3 of 5% glucose solution to tube A, sucrose solution to tube B and maltose solution to tube C 3. Add three drops of Janus Green B indicator to each boiling tube and gently shake to mix the contents 4. Add a thin layer of liquid paraffin (2mm thick) to the top of each boiling tube 5. Leave the boiling tubes until the Janus Green B indicator has changed colour from blue to pink 6. Insert the bung in tube A and stand it in the water bath. The clip should be removed or open. Leave the tube to equilibriate for 5 minutes 7. Close the slip to seal the apparatus and mark the level of the meniscus 8. Use a ruler to mark another line 10mm higher than the miniscus 9. Start the stopclock and time how long it takes for the miniscus to move from its original positition to the 10mm line 10. Repeat 3 times 11. Work out rate of respiration of the different sugars by taking 1000 divided by the mean time for each sugar
for anyone doing aqa a level biology pmt.physicsandmathstutor.com/download/Biology/A-level/Notes/AQA/Practical-Skills/RP%2004%20-%20Investigating%20Cell%20Membrane%20Permeability.pdf heres the risk assesments and what not.