The wireframe Queen heads are almost certainly a reference to the wireframe Sigma virus from Megaman X2 and X3. Same colours as the second palette in X2, same method of appearing as X3.
The wireframe effect Immediately made me think of X2 and 3. Maybe I had just played it recently, but it felt like there was a lot of inspiration taken from the games.
The hype 'tho, when I saw that before meeting Queen and when we first start chapter 2 with "DELTARUNE" popping up and then "Chapter 2" appearing below it with Queen's laugh... Toby is awesome, always making people lose their shit just because of his smart and effective design choices!
This freaked me the hell out, it's never happened to me before! To anyone reading, I think this occurs when you're reloading the game after saving in the room with the arcade machine after its been destroyed?