Hey, you are correct. We did mistake in the subtraction, though it is a silly mistake. We are sorry for our mistake. And thanks to you for the observation.
Does the stock solution necessarly have to be in repose for 3 days? or Can we heat it up to make the hydrolysis faster? How can we accelerate the reaction without using enzymes? :) thank you!
Hi. Nice video. I follow your method. But my sample titration reading is higher than blank titration reading. How to solve this issue? If we subtract blank and sample tiration volume, it will become negative.
I observed this method. i dont unterstand WHY during the sample titration you used the working standart solution as titrand used before IN BLANK TITRATION . In the method Lane Eynon THE TITRATION OF THE SAMPLE SOLUTION MUST BE DONE WITH THE SAMPLE SOLUTION AS TITRANT SO YOU HAVE USED THE WORKING STANDART SOLUTION(2.5MG/ML) TWICE. IS THAT CORRECT?
Thank you! Your nice voice is more clear without the loud music. Would it be a lot to ask not to play the music? Your videos are amazing, clear, descriptive and super helpful! You're very much appreciated!!
Hello., i tried your method and the color does not chnage nack to orange red after addition of 1% methylene blue. The blue color would not go away even after adding more than 26ml for working standard titration. Not sure what the issue is. Also, could you let me know your email to contact you directly
Hello. I hope you can answer me. I am developing a method to help me analyze the total sugars in molasses to implement it in the quality control laboratory of a company that produces Rum. I am a practicing student so that would be my contribution that I would leave to the company, I have looked for many methods but it must be a method that does not involve many reagents since I must work with the reagents they already have in the laboratory. Furthermore, being a company, its analyzes must be, so to speak, quick to carry out. This Lane-Eynon method seems appropriate to me because it is the first experiment I have seen that does not use many reagents and is quite simple to perform. If you have any suggestions on how to determine the total sugars in molasses, please I would appreciate your response.
Excuse me ! I want to know about the sugar standard , so why you choose sucrose for standard and Is it possible if prepared another sugar like (maltose fructose…)
Hello, Thanks for such a detailed and understanding video... Great work... This helped a lot.. It would be very very helpful if you could please share the official reference for this procedure along with calculation, as I have to include this in my thesis...
thank you for this detailed video..., the first thing to ask on preparing the heating plate, at what temperature the pre-heated plate should be maintained also can we use this method for identifying total sugar in wheat or rice straws?...if any recommendations, and also you used sucrose in the stock solution preparation which is non-reducing sugar why is that since we are determining a reducing sugar ?... thank you
1. No specific temperature for hot plate. Use the temperature enough to boil the solution. 2. You can use this method for food sample only. 3. Sucrose is converted into reducing sugar while kept for 3 days in HCl.
Thank you for your video , I have question what do you mean by the concentration of original sample 60% when you add 5-7ml of the honey sample , i didn’t get it
Expected sugar concentration is 60%. That's why we added 5-7ml sample. Because sample amount varry depending on the expected sugar concentration in your sample
Thanks for this video. Please if you say that this procedure is the same for estimating the dextrose equivalent, then why did you say it's the estimation of both reducing and non-reducing sugar? Dextrose is reducing only.
Hello, is it the same procedure for dextrose equivalent determination?? Or you have another procedure because i need to test the dextrose equivalent for invert sugar? Please advise
I have a problem with the analysis of the percentage of fat , the results of one sample are different when i test it twice, whats the matter?! I am using gerber method … thanks for helping
After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor
Hey there! I have a question: should it be possible to do this determination on a sirop which contains invert sugar (which is a mix between the same amount of glucose & fructose molecules)? The amount of invert sugar in the flask is unknown obviously, that's why I would want to do this experiment :). I do not know what the expected sugar concentration is atm, but I could calculate it because I already did a polarimetry on it. The sirop also has a light-brown color, if that even matters. Anyway, any help would be hugely appreciated!
@@MicroChemsExperiments I see. Thank youuu. Then how about For sucrose, it shall be dried for how many hours and at what temperature before doing stock standard solution?
Hello, thank you for the video it really helpful. I have a question about sample preparation, is it the procedure can be use to other method? Because i want to do phenol-sulfuric acid method with solid sample (biscuit). Thankyou!
Ok thanks. I tried your method, however my analyte is not as thick as yours. Hence it is difficult to see the colour change. Do you know the reason why?
@@huilynmak5928 Colour change depends on sugar concentration. Sample colour does not influence on the titration colour. Please perform standard titration and try to understand the color change properly. Then do the sample titration following my video strictly. You will get the appropriate results.
@@MicroChemsExperiments thanks! I have a few more questions to ask. What happens if I don’t get a complete bright orange colour after heating for 3 min? I got a bright orange ppt with tint of blue during my experiment. Is this method possible to determine sample with unknown %DE? Thanks in advance!
After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor
Thanks for this. Two questions quickly: 1. Is it best practise to use a measuring cylinder to measure the stock solution used to make the working standard solution or to use a pipette or at least a burette? 2. What's the reference method used?
After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor
After filtration of sample solution, we took 50 ml sample solution to 100ml volumetric flask. This 50ml is used in the calculation to find the dilution factor
@@MicroChemsExperiments sir I kwn this is lane eynon method but as per fssai manual is Too tough test but apne jo krvaya vo muje bht acha but iska written material kha se milega jiska reference de ske plzz urgent reply
