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Total Yeast & Mold Count (Total Fungal Count)_A Complete Procedure (BAM, Ch-18) 

MicroChem's Experiments
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29 сен 2024

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Комментарии : 92   
@md.shahinsarkar1625
@md.shahinsarkar1625 Год назад
Good video
@shubhamsorate8918
@shubhamsorate8918 Год назад
Excellet video
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thank you very much!
@sonandpets1215
@sonandpets1215 2 года назад
I haven't received notifications for your posts in a while. I missed you, your team, and your videos! Thank you! Welcome back!
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
So glad to hear this. Actually we were so busy in our professional life. Now, we are back. Stay with us.
@davoudsalehi8386
@davoudsalehi8386 11 месяцев назад
Sehr gut 👍
@sibamdatta9723
@sibamdatta9723 10 месяцев назад
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
@nainamohamed6727
@nainamohamed6727 2 года назад
Is there any chemicals to prevent yeast and mould in packeged drinking water
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Yes. benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate are used to prevent the growth of yeast and mould
@willdrin2851
@willdrin2851 2 года назад
Can you use buffered peptone water instead of 0.1% peptone water?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
You can
@jkgou1
@jkgou1 11 дней назад
Would you please instruct us: The sample on each plate is 100μl(0.1ml) Do we need to multiply the final result with 10 for 1ml sample for this spread plate method ? We use 1 ml sample on each plate for pour plate method but we use 0.1 ml sample on each plate for spread plate method.
@sibamdatta9723
@sibamdatta9723 10 месяцев назад
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
@Mmababy24
@Mmababy24 4 месяца назад
37
@ammardilawar510
@ammardilawar510 Год назад
Thanks for the excellent video. I have few questions 1. What can we use instead of stomacher bags ? 2. How to prepare sample if we have swab samples or environmental samples ? 3. Formula if we have n3 or n4 also ?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
1. Large duran bottle 2. Dissolve the swab stick in 10ml buffer solution. After shaking and vortex, dilute if necessary. Then analyze. 3. First calculate average from 2 plates. In this way, make 2 values from 4 plates.
@firasabili5142
@firasabili5142 6 дней назад
Is this method same with ISO 16212:2017?
@sibamdatta9723
@sibamdatta9723 10 месяцев назад
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
@dennarkoe5928
@dennarkoe5928 3 месяца назад
26 degree for mould yeast with slowly incubation 5 day
@queeniedalisay9970
@queeniedalisay9970 2 года назад
Hi, is okay to use potato dextrose agar? with same procedure?
@kelvindanntobias5642
@kelvindanntobias5642 7 месяцев назад
Yes.
@sujonali7125
@sujonali7125 10 месяцев назад
Thanks for procedure
@justacommonman7000
@justacommonman7000 2 года назад
What is meant by BAM in the title?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Bacteriological Analytical Manual
@srikanthsri9442
@srikanthsri9442 2 года назад
Kindly upload the Enumeration technique of Bacillus Coagulans
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Noted
@sibamdatta9723
@sibamdatta9723 10 месяцев назад
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
@Haokip_Lunjapikai
@Haokip_Lunjapikai 5 дней назад
What are the other media that can use other than DRBC agar??
@MicroChemsExperiments
@MicroChemsExperiments 4 дня назад
You can also use "Yeast Extract Chloramphenicol Agar"
@Haokip_Lunjapikai
@Haokip_Lunjapikai 4 дня назад
@@MicroChemsExperiments thank you
@santoshrock1943
@santoshrock1943 9 месяцев назад
Can u please tell ........What is the formula for calculation if colony count in one successive dilution is less than 150?
@ruqiyakhamroon4434
@ruqiyakhamroon4434 Год назад
Can we use potato Dextrose Agar instead of DRBG Agar
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes you can but you need to add chloramphenicol as a supplement to make it more selective for Yeasts & Molds.
@ruqiyakhamroon4434
@ruqiyakhamroon4434 10 месяцев назад
R can we use potato Dextrose Agar w/chloramphenicol
@karthikpharmacist5271
@karthikpharmacist5271 Год назад
Nice teaching mam
@MicroChemsExperiments
@MicroChemsExperiments Год назад
It's my pleasure
@rakeshbodhe8460
@rakeshbodhe8460 2 года назад
Please do the video of residue free chlorine
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Noted
@christopherkambondo4806
@christopherkambondo4806 2 года назад
this I waited for long, thanks
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
I made it for you
@sona.r1662
@sona.r1662 7 месяцев назад
Why we use diluent instead of water
@QualityControlLab-d6s
@QualityControlLab-d6s 10 месяцев назад
one more issue media are not solidified
@manarasmr6946
@manarasmr6946 3 месяца назад
Well explained thanks
@dessienatalia1193
@dessienatalia1193 Год назад
Hello. Does pour plating method can be used for yeast and mold culture?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
In that case you have to use SDA media for pour plate
@olabisiolatundun3696
@olabisiolatundun3696 Год назад
Please can I use thesame technique and culture Media for pharmaceutical samples?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes. You can
@RubenNdosiba
@RubenNdosiba 8 месяцев назад
merci pour la video
@manojpheiroijam766
@manojpheiroijam766 2 года назад
Please upload video for Staphylococcus aureus and Enterobacteriaceae count
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Noted
@mumuhay2345
@mumuhay2345 Год назад
is there any video for bacterial count?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes. Link: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-IlTnLEgivts.html
@neethuaneethua941
@neethuaneethua941 Год назад
Can we use Butterfield phospate buffer instead of peptone water
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes you can
@neethuaneethua941
@neethuaneethua941 Год назад
​@@MicroChemsExperiments thank you ❤
@nainamohamed6727
@nainamohamed6727 2 года назад
How to control yeast and mould in packeged drinking water
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Add one of these chemicals benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate.
@nainamohamed6727
@nainamohamed6727 2 года назад
@@MicroChemsExperiments thank you for your kind reply one more doubt sir how much qty add per litre sir
@ruqiyakhamroon4434
@ruqiyakhamroon4434 Год назад
I haven't received any reply
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Sorry for late reply
@Ninxh678
@Ninxh678 Год назад
Hi! Great video. Just want to ask did the sample weight change from 25g to 50g?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
The question is not clear. We took 25g sample for testing
@Ninxh678
@Ninxh678 Год назад
@@MicroChemsExperiments because according to the FDA-BAM Chp 1 the sample weight stated to be 50g. So I just want to ask did the result for 25g different from 50g?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@Ninxh678 Result will not differ.
@Ninxh678
@Ninxh678 Год назад
@@MicroChemsExperiments Thank you!
@ayakhaled2474
@ayakhaled2474 7 месяцев назад
Incubate plates in upright or invert position
@MicroChemsExperiments
@MicroChemsExperiments 7 месяцев назад
Invert
@ayakhaled2474
@ayakhaled2474 7 месяцев назад
But in iso method 21527-2 is written: 9.2.3 Incubate the prepared plates aerobically, lids uppermost, in an upright position in the incubator at 25 °C ± 1 °C for 5 d to 7 d. If necessary, leave the agar plates to stand in diffuse daylight for 1 d to 2 d.
@sorngsinadane8447
@sorngsinadane8447 Год назад
Excuse me, can I get the reference of calculating formula?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Reference is given in the video title
@sorngsinadane8447
@sorngsinadane8447 Год назад
@@MicroChemsExperiments Many thanks to you. Keep this good work always!☺️
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@sorngsinadane8447 Thank you so much.
@arlhenpinera1884
@arlhenpinera1884 Год назад
Do you still need to add antibiotic solution in the media?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
No. No need to add antibiotics in DRBC agar media
@arlhenpinera1884
@arlhenpinera1884 Год назад
@@MicroChemsExperiments thank youu
@gousiagulzar7002
@gousiagulzar7002 Год назад
Can we use blending jar instead of stomacher machine
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Contamination can occur in blender jar. So you can shake by your hand....or you can use vortex mixer
@gousiagulzar7002
@gousiagulzar7002 Год назад
@@MicroChemsExperiments thank you
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@gousiagulzar7002 welcome
@mohdabrar2839
@mohdabrar2839 Год назад
thanks
@MicroChemsExperiments
@MicroChemsExperiments Год назад
You are welcome
@sambathsocheata1389
@sambathsocheata1389 Год назад
Can we use this method for determination of yeast and mold?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes
@sambathsocheata1389
@sambathsocheata1389 Год назад
For enumeration need 6 falcon tube then how about determination do we still use 6 falcon tube or just use 2 tube only ?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@sambathsocheata1389 you can use only 2 tubes. It depends on the expected concentration of yeast and mold in your sample
@sambathsocheata1389
@sambathsocheata1389 Год назад
Thank you ❤
@julytran861
@julytran861 2 года назад
👍
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Thanks
@sorngsinadane8447
@sorngsinadane8447 Год назад
Hi, I am wondering what is the purpose of labeling 2 trial plats? eg: DRBC10-2 / trail -1 and DRBC10-2 /trail -2. Thanks in advance.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Contamination can occur in every steps of any microbiological test. So double trial can increase your confidence level of the test.
@m.luqmanulhakim2672
@m.luqmanulhakim2672 2 года назад
what is the different about bacteria and yeast in Macroscopic ? I am still confused. Thank you
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Study more in Google resources
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