Deviations from Beer-Lambert law 

Non-linearity can be observed in the absorption curve due to deviations from Beer-Lambert law. These deviations arise from analysis of sample having higher concentrations of the analyte or else the may be related to spectral and chemical aspects. In this video, we will see three types of deviations.
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0:00 Introduction
0:10 What is Beer-Lambert law?
1:32 Deviations from Beer-Lambert law?
3:43 Refractive index (n)
4:54 Real Deviation
6:38 Spectral Deviation
9:57 Selection of monochromator
11:28 Chemical Deviation