Diagnosis of Syphilis - Types of tests, Principle, Uses, Short-comings 

Hi. With this video, we will discuss the various diagnostic tests for syphilis and be better prepared for treating our patients. This video is directed towards dermatologists and is not at all advisory for non-medical personnel.
Introduction - 01:50
Issues with diagnostic tests - 05:03
Diagnosis - 07:17
Dark Ground Illumination - 08:56
Dark Ground Illumination: Procedure - 13:16
Dark Ground Illumination: Pitfalls - 14:50
Direct Fluorescent AB - ABS - 16:27
Direct Fluorescent AB - ABS: Procedure - 19:40
Direct Fluorescent AB - ABS: Pitfalls - 21:22
Polymerase Chain Reaction - 22:32
Electron Microscopy - 24:36
Serological Tests - 25:22
Serology - 28:22
Wassermann Test - 32:50
VDRL - 35:35
VDRL: Procedure - 38:16
VDRL: Biological False Positive - 39:45
VDRL: Prozone Phenomenon - 42:14
Rapid Plasma Reagin - 48:35
Rapid Plasma Reagin: Procedure - 49:50
Rapid Plasma Reagin: False negatives and positives - 51:06
TRUST - 51:45
Treponemal Tests - 52:52
Enzyme Immuno Assay - 54:54
Chemiluminescence immunoassay - 56:41
TP Hemagglutination Assay - 58:23
TP Particle Agglutination Assay: Procedure - 01:00:53
Multiplex Flow Immunoassay - 01:01:42
Fluorescent antibody-Absorption - 01:03:06
Immunoblot Assay - 01:05:00
Point of Care Tests - 01:06:07
NAAT - 01:09:39
Reverse Sequence Algorithm - 01:10:33
Further reading - 01:15:68
End - 01:16:32
Good articles on Congenital Syphilis:
1. Chapter 36 - Infectious Syphilis. In: Sexually Transmitted Infections, 2ed. Pg-429, Gupta S, Kumar B. ISBN: 978-81-312-2809-8
2. Satyaputra F, Hendry S, Braddick M, Sivabalan P, Norton R. 2021. The laboratory diagnosis of syphilis. J Clin Microbiol 59:e00100-21.
3. Nayak S, Acharjya B. VDRL test and its interpretation. Indian J Dermatol. 2012 Jan;57(1):3-8. doi: 10.4103/0019-5154.92666. PMID: 22470199; PMCID: PMC3312652.
4. Lum B, Sergent SR. Rapid Plasma Reagin. 2023 Jul 25. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. PMID: 32491664.
5. Young H, Moyes A, Seagar L, McMillan A. Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis. J Clin Microbiol. 1998 Apr;36(4):913-7. doi: 10.1128/JCM.36.4.913-917.1998.
6. CDC URL: www.cdc.gov/std/treatment-gui...

Развлечения