The difference between HPLC and UHPLC is significant in the analytical laboratory. HPLC has particles of 3-5 micron, while UHPLC has particles of 2 micron or less. UHPLC has smaller column dimensions, lower flow rates, higher backpressures, and narrower detection parameters. HPLC is commonly used in routine environments, while UHPLC is more common in research and development. UHPLC offers faster separations, better resolution, lower costs, and higher throughput. For example, a separation took 2.5 minutes using a column with 4.0 µm particles, while a separation took 1.5 minutes with 1.5 µm particles. The speed also allows for better solvent usage and disposal costs. Both HPLC and UHPLC are commonly used in routine environments, but their differences are not exclusive.
12 окт 2023
