It's really a great video and so clear explanation... This channel deserves to be followed and subscribed.. I hope you will make new videos always in genetics, genetic engineering, gene therapy and so on , thank you so much.
My uni lecturer had me thinking this was impossible to understand, you’ve made it so clear and simple in 11 minutes, thank you som ugh for all the work you put into these.
Actually i am a bit confused before watching your video as I studied from my coaching notes 😅 but after watched your video I feel blessed. You make this concept so easy peasy ❤️
Some of us have a difficult time understanding different accents but your English is very clear and almost every word was easy for me to understand. I've lost some of my hearing and had a difficult time hearing the word you used @1:14. I have little to no experience with DNA and more than likely the reason I did not understand that word. Your work is obviously helping others to understand better. I hope you continue to teach as you are very good at this. ❤
Thank you Priscilla 😊, the word was lesion that is an area of abnormal tissue. English is not my first language, maybe I pronounced it wrong. Thank you for your support 🙏
@@EasyPeasyLearning OK I get it now and thank you for responding. The word sounds like "le´zhun" according to dictionaries. English is my only language but I often get words wrong especially when it comes to the sciences. You've done a lot better than I would have!
Thank you for your comment, these are different sites to have more insight about the topic www.nature.com/articles/cr20081 blog.addgene.org/crispr-101-homology-directed-repair
Thank you for the detailed explanation. Just a query, from the cell's viewpoint, why not use homologous recombination as far as possible if it so less prone to loss of DNA? What would make a cell choose NHEJ?
Hi Prabal, in non-homologous pathway the part of DNA that can still be use as template will first follow the homologous recombination pathway but if the strand have mutations and can not be use as a template then it will shift itself on the non homologous pathway.
Hi Pratik, HR leads to accurate repair, while NHEJ is intrinsically mutagenic but NHEJ is a faster and more efficient DSB repair pathway than HR. So both pathways have there own benefits and disadvantages. The Reference Link for this statement is pubmed.ncbi.nlm.nih.gov/18675941/
This a great explanation thanks but really worrying having had 6 CT abdominal CT scan within 3 years due to digestive issues .... How could my DNA take that much of X ray and repair efficiently ? Really worried and will appreciate any answer
Hey thank you for this amazing lecture But I have a question If the break was in the 5% part that is not similar in the homologous chromosome what's gona happen then ??????
How often does dna double strand breaks in normal days (not by medical radiation/xray/ct scan)? How to know if a ct scan double strand break has been repaired correctly?
Hi Henry, all information provided on our videos is from books or proven articles. Which information do you think is provided incorrectly? We can look into the matter and correct if required.
@@EasyPeasyLearning By proven articles you mean peer-reviewed articles? a link to those would be excellent. Some information is misleading or not correct, for example, you mentioned DNA double-strand damage occurring primarily from radiation, of which the most common source is nuclear bombs.. source? I am sure that is not correct. The most well-known cause of DNA double breaks is radiation and chemotherapy, but also endogenous processes like replication. In addition to this, the common source of Ionizing radiation is radon gas or UV exposure, even some medical equipment... not a bomb. You never mention endogenous sources of DNA double-strand break coming from lymphocyte differentiation or replication errors. In addition to this, you mention Mre11 Rad50 and Nbs1 being just 3 proteins instead of being a heterodimer complex of M2R2N2. You also mention the 3' end resection overhang is 1000bp, and I would really like to see a source for that. In my experience end resection is highly variable. In addition to this, Ku proteins and MRN complex can funciton in both NHEJ and HR. The pathway choice is dependent on environment and cell stage... along with a couple other factors.
Hi Henry! Thank you for the feedback. We will looked into the matter and asked regarding about the information you gave. The answers are given below: Atomic bomb were suppose to be used as a reference of radiation here and should have not been mentioned as a primary cause. We will correct this information accordingly. The UV radiations that you mentioned is not mentioned in this video because this video is a second part of our previous video. We will correct this mistake and rename the videos as part 1 and part 2 The link to the previous video is: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-oRfK85gPAVA.html The 3' overhang value was not mentioned as a solid fact but was rather intended to be given as an approximation. We will edit that portion to include approx symbol to clear the misunderstanding. The ku proteins you mentioned are not included to keep the video short and simple. Thank you for your time and feedback.
