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DNA extraction from Blood 

Centre for Proteomic & Genomic Research
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CPGR training video for DNA extraction from blood including workflows and protocols, highlighting instrumentation and consumables needed.

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4 май 2016

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Комментарии : 135   
@MJ-ye7dd
@MJ-ye7dd 3 года назад
The best ever, so professional and clear.
@samrakhan4408
@samrakhan4408 6 лет назад
So well explained!
@priyam8261
@priyam8261 6 лет назад
Thank you very much. Nicely explained
@evaberger1628
@evaberger1628 6 лет назад
So helpful! Thank you
@pseudolichiacoliaerogenes3451
Thank you for this amazing and well organized vid Professor!
@NazriB
@NazriB Год назад
Lies again? Project Artemis Blood DNA
@medicalacademy8135
@medicalacademy8135 3 года назад
excellent presentation many thanks
@datisalaee4693
@datisalaee4693 9 месяцев назад
Very helpful presentation. Thank you.
@mhdamins
@mhdamins 3 года назад
amazing thank you so much
@liveisnice9510
@liveisnice9510 6 лет назад
Thank you doctor
@erinwusiblessing9262
@erinwusiblessing9262 Год назад
Very helpful Thank you so much.
@natpethunai3964
@natpethunai3964 4 года назад
great job docter
@qaisariqbal6758
@qaisariqbal6758 2 года назад
Superb explanation
@user-lx5pu4je7m
@user-lx5pu4je7m 6 месяцев назад
Thank you so much 🥰
@noman12310
@noman12310 4 года назад
Thank you
@mouhamadoumoustaphadiop8024
@mouhamadoumoustaphadiop8024 2 года назад
I am very glad for this movie Thanks
@muhamadjameel1517
@muhamadjameel1517 6 лет назад
appreciating ,,, hafiz waheedullah
@r4rallinone64
@r4rallinone64 6 лет назад
Thanks you
@nandadeep999
@nandadeep999 Год назад
Very well explained. Please give little brief about its applications in pharmaceutical
@ms.kalico4489
@ms.kalico4489 Год назад
That was a very good video!
@ammarkareemrashidghareeb5762
@ammarkareemrashidghareeb5762 3 месяца назад
excellent video. thanks a lot.
@snehajpraj9100
@snehajpraj9100 6 лет назад
Thank you....
@deepalibhardwaj2696
@deepalibhardwaj2696 6 лет назад
your welcome
@hananeboukrid880
@hananeboukrid880 4 года назад
thanks
@EmmanuelAsiimwe-ey6fj
@EmmanuelAsiimwe-ey6fj Месяц назад
Well organised and presented
@harshkumaragrawal5278
@harshkumaragrawal5278 3 года назад
THANKYOU SO MUCH MAM I AM TRYING TO THE BEST PERFORMES
@DarlinMarleneVeVo
@DarlinMarleneVeVo 3 года назад
nice, thanks
@moamenmetwelly1898
@moamenmetwelly1898 6 лет назад
Great work👏👏
@user-bd4cv7ew9z
@user-bd4cv7ew9z 7 месяцев назад
Awesome
@hazlanshah
@hazlanshah 6 лет назад
Hi. May I know how do you create the animation in the video?
@hamidizouina4787
@hamidizouina4787 6 лет назад
MIS7 i am asking same question
@azlieabd
@azlieabd 3 года назад
I loveeee it.
@huntergaming9793
@huntergaming9793 2 года назад
I fell in love with you lady how well you explained it 😘😘
@moemoethecoolest
@moemoethecoolest Год назад
I don't know how I got here because I'm just a regular person but I still think this is cool. I want to study biology. I wish I could work in a lab but I'm very clumsy so I think I'll be a writer instead.
@parsasanjana7813
@parsasanjana7813 7 лет назад
Hello, when you draw blood from a volunteer, should the samples be kept in ice, in case, one has to travel from the hospital to the lab? ( to avoid DNA degradation). I am confused since I read direct contact of blood with ice causes damages WBC.
@priyankaflorina8411
@priyankaflorina8411 7 лет назад
you should keep it on the ice after 10-15 minutes prior to withdrawal.
@noorpk
@noorpk 6 лет назад
You may use lysis buffer, 2X concentration, then you can keep the it for longer times
@khushboogautam6730
@khushboogautam6730 Год назад
Hii ma'am I have done as same all the procedure with my blood sample but my conc is very low like 5 and 7 ng/ul and one more thing some time ratio is above than dna means rna
@droneasmr
@droneasmr 4 года назад
This may be a stupid question, but is this is DNA extraction from the white blood cells of the blood component, right?
@lavanyamadhusoodanan3432
@lavanyamadhusoodanan3432 3 года назад
If I add proteinase k just the double amount..suppose 40ul instead of 20ul.. to blood during DNA extraction..then what happens?
@drproductiontv8593
@drproductiontv8593 2 года назад
GREAT
@Tepmodify
@Tepmodify Год назад
Good video.
@rizkard311
@rizkard311 Год назад
So is it okay if the blood sample was lysis in the EDTA tube?
@heshamalsayaghi1013
@heshamalsayaghi1013 5 лет назад
Dear:what mean Operation Profile (OR) . In blood Groub . O rhD Positive. Please tell me if you have experince about this.
@The_Eminence
@The_Eminence 3 года назад
very interesting
@shimashima8585
@shimashima8585 4 года назад
I like it i need a PDF of it.
@Somehackcopiedmyuser
@Somehackcopiedmyuser 2 года назад
My Centrifuge max spin is 7000 RPM, will this be sufficient for this protocol?
@tanmoysaha2490
@tanmoysaha2490 6 лет назад
nice.
@josephwhite4933
@josephwhite4933 5 лет назад
Am I able to just use a centerfuge
@patrickchibwe8716
@patrickchibwe8716 Год назад
Great
@zebafatima4462
@zebafatima4462 5 лет назад
Maam we use ssc(mixture of sodium acetate nd EDTA) buffer instead of proteinase k
@ahmetziyaaktas9179
@ahmetziyaaktas9179 6 месяцев назад
So nice
@Daniel_loung
@Daniel_loung 3 года назад
wow, i am so Passionate with this, but i cant do what i want because i have anxiety disorder, i always shaking hand during the experiment, i got rejected from other laboratory so many time, anyone can share experience or advice for me. Best regard .
@chemistryinstruments7156
@chemistryinstruments7156 3 года назад
Maybe you can see a neurologist first to get an idea what is going on. I had some problems with that also, some lab stuff needs some finesse and it makes me nervous sometimes. Drugs are an option too for anxiety. I'm sorry it may involve doctors which are expensive sometimes.
@markgearing
@markgearing 2 года назад
Identify what it is about this process that you’re passionate about. Is it working in medicine? Is it assisting in treatment? Is it process? Something else? Once you know what inspires you look for alternative roles with similar inspiration in which your anxiety isn’t detrimental to the outcome. Addressing your anxiety will be helpful in many aspects of life, but it’s a big world with many roles available. You don’t have to do that job; find one that you’re ready for. And remember, whatever you start with is just a stepping stone to your next role. You can work on reducing your anxiety and expand your skill base at the same time. Or one at a time. Whatever works for you.
@sunnymadar4056
@sunnymadar4056 Год назад
Learn art of living workshop do sky process with me also same thing happened but after practising sky technique I got selected and that is now thing of past , I got good job too 😌
@hrahselmuanchhana8359
@hrahselmuanchhana8359 Год назад
Smoke weed everyday
@nwaobigift9924
@nwaobigift9924 11 месяцев назад
Just try to be bold and focused. Don't be scared of making mistake. When your mind is comfortable and not scared, your hands won't shake. Pron you're afraid of the people in your surroundings. You have to work on that. Train yourself to not be scared of them. Because you're all learning and everyone makes mistake so please just try to first grow ur confidence.
@Tepmodify
@Tepmodify Год назад
Clot in column tube. Trableshooting
@yenantruong987
@yenantruong987 Месяц назад
Thanks for the creative video, kindly you please share the ingredients in the buffer AL, AW1, AW2
@paulidam6582
@paulidam6582 3 года назад
Nice
@harrison7952
@harrison7952 6 лет назад
How do i modify this protocol to extract DNA from dried blood spots?
@kingofnuclearfallout39
@kingofnuclearfallout39 3 года назад
Go to some DIY video type in gene editing DIY it's easy just the typing part good luck
@amirulafiq1365
@amirulafiq1365 6 лет назад
nice
@leah8238
@leah8238 5 лет назад
What kit do you use?
@MJ-ye7dd
@MJ-ye7dd 3 года назад
I saw QIAamp DNA Blood Mini Kit
@sharifulislamhridoy4122
@sharifulislamhridoy4122 4 года назад
best
@erysaputra8995
@erysaputra8995 3 года назад
is this the same procedure used for extracting DNA of SARS-CoV-2 diagnosis with PCR?
@sumaiyamansoor7784
@sumaiyamansoor7784 2 года назад
No. The PCR test for SARS-COV2 requires RNA extraction from blood since the virus is an RNA virus it does not have any DNA.
@mehrunnisaarif2191
@mehrunnisaarif2191 5 лет назад
how to prevent the lysate in purification column from thickning?
@zapp1984
@zapp1984 Год назад
Anti coagulant in the draw tube. Should be already taken care of before you get the sample.
@nikita8927
@nikita8927 3 года назад
which method is this??
@lianaa8629
@lianaa8629 Год назад
What is the size of DNA ladder?
@nexigent
@nexigent 2 года назад
I want to look at my own dna from my blood how can i do that?
@Lifescience_aspirant
@Lifescience_aspirant 5 месяцев назад
Please make a video on DNA isolation from saliva samples
@sheereen-gy9ih
@sheereen-gy9ih 11 месяцев назад
How the specific gene or species chromosomes whats getting affected in human
@linamahmoud651
@linamahmoud651 5 лет назад
so isn't it written in the SOP ?? what is the benefit of watching this video '?
@user-rl8cc4qq9i
@user-rl8cc4qq9i Год назад
❤❤❤❤❤❤
@francestolentino9396
@francestolentino9396 4 года назад
"Running buffer in this case is 1 times TBE containing a 30 ????? is added to the tank" at 9:13
@kristtyguevara7497
@kristtyguevara7497 3 года назад
Ethidium bromide
@marwanj.r2513
@marwanj.r2513 3 года назад
Wow my alaah bless u 🤩🤩🤩🤩
@samanaguib5063
@samanaguib5063 3 года назад
im watching this for fun for no reason
@ayeshanawaz4206
@ayeshanawaz4206 5 лет назад
what are consumable in DNA extraction
@JoeMama-kg9lx
@JoeMama-kg9lx 4 года назад
Esha Nawaz the dna is, but make sure to let it dry
@headdragondavidaustinsimmo4025
@headdragondavidaustinsimmo4025 4 года назад
i need blood test
@apol8245
@apol8245 4 года назад
ay yo next time i bleed ima take some dna out: 🧬
@headdragondavidaustinsimmo4025
@headdragondavidaustinsimmo4025 4 года назад
in Bakersfield California there my grandfather's skull chopped in half worth millins is Samuel prophet reborn
@xcp4518
@xcp4518 3 года назад
Thanks David very cool
@sashamuller9743
@sashamuller9743 4 года назад
where can i learn genetics to be able to understand this?
@victoriarichard3468
@victoriarichard3468 3 года назад
Same with I, honestly seems I will save probably will be useful in future
@ImNotQualifiedToSayThisBut
@ImNotQualifiedToSayThisBut 2 года назад
Pharmacy, chemistry, biology, biochemistry etc. Enter university or learn from free resources online. Some universities offer their lectures for free only downside being you can't actually attend exams and graduate but for autodidactic learning there's enough resources.
@derek3052
@derek3052 3 года назад
How to extract DNA out of a bone Can we really extract DNA from bone or not can you please tell me
@TF2Scout..
@TF2Scout.. 3 года назад
We can
@derek3052
@derek3052 3 года назад
@@TF2Scout.. Oh ok thx
@IamAshokKumarSingh
@IamAshokKumarSingh 2 года назад
How I found dna fo onion plz make a video
@aliarshadali8019
@aliarshadali8019 5 лет назад
السلام وعلیکم ورحمتہ اللہ وبرکاتہ ھو جناب محترم کیا انسان کے جسم کے پسینے سے ڈی این اے ٹیسٹ سے جنس عمر نشوونما اور غذائیت زنا اور دوسری چیزوں کو با آسانی معلوم کیا جاسکتا شکریہ
@wingsofgalaxystarunlimited6160
@wingsofgalaxystarunlimited6160 3 года назад
ssoooo pro
@GursewakSingh-py4wi
@GursewakSingh-py4wi 2 года назад
how much it expensive process. please tell the price.
@Sharkyktc001
@Sharkyktc001 2 года назад
A qiamp DNA kit has enough reagents for 50 extractions, costs $259. So excluding the quality control steps and other consumables used, that's $5.18 per sample.
@gabrielavelandia526
@gabrielavelandia526 5 лет назад
No entendí
@pitou4108
@pitou4108 3 года назад
kumpliketed
@silviaalexandrecordeiro8400
@silviaalexandrecordeiro8400 2 года назад
🌹🌎🌍🌏🌹
@naimjelahastu3669
@naimjelahastu3669 3 года назад
is there any lab report for this hehe I'm just asking for friend
@ayeshaafzal8450
@ayeshaafzal8450 3 года назад
Yes
@zainabafzal9613
@zainabafzal9613 2 года назад
@@ayeshaafzal8450 can you please share that report with me. I urgently need it😬❤️
@zyyma6802
@zyyma6802 2 года назад
@@zainabafzal9613 same hereee
@zainabafzal9613
@zainabafzal9613 2 года назад
@@zyyma6802 I have it. I can send it to you. Just give me your email or something
@Arbulatov
@Arbulatov 6 лет назад
What can you do with extracted DNA?
@amortality999
@amortality999 5 лет назад
eat it and youll have the guys powers?
@akumar7298
@akumar7298 5 лет назад
Ratna singh
@sandeshbme
@sandeshbme 4 года назад
Extracted DNA is used in applications related to molecular diagnostics, Genetics, Forensics, Even in developing vaccines like for eg corona virus
@abdulezizmuhemmed6279
@abdulezizmuhemmed6279 3 года назад
u can use it for DNA fingerprinting and capture the criminals.
@ankitbarnwal4328
@ankitbarnwal4328 4 года назад
Wats this aw1 and aw2??
@Hey_reema
@Hey_reema 3 года назад
Wash 1 & wash 2
@ragavipriya08
@ragavipriya08 4 года назад
It will take 1yr ... 4me to learn this lengthy... Technique😆😂😂
@StevenJones-bl4py
@StevenJones-bl4py 11 месяцев назад
z-DNA @0:00
@sailplaneFan707
@sailplaneFan707 2 года назад
I am going to interrupt incidentally
@yusufucar8604
@yusufucar8604 4 года назад
Bru i study linguistics what the fuck on earth am i doing here at 3 am
@headdragondavidaustinsimmo4025
@headdragondavidaustinsimmo4025 4 года назад
doctor said 200.000 in 80s i seen it im telepathic i asked him to bring me and kids witnessed it 30 kids
@sharmeensarfraz8198
@sharmeensarfraz8198 Год назад
Why Again and again repeat same process 🥺🥵🥵
@AndruideAtario
@AndruideAtario 4 года назад
"please note that blood sample collection should always be performed by a --- plubottelist --- ?" What the fuck, not even 30 secs into the video and already fucked up.... :((((
@mominjaved7616
@mominjaved7616 2 года назад
Don't use music at background.. Its haram in Islam
@headdragondavidaustinsimmo4025
@headdragondavidaustinsimmo4025 4 года назад
im in gold beach Oregon i have b+ blood half DNA of man im adam yoseph Joseph Moses Ishmael Gidian im Jesus Bethlehem son of David Mike Simmons died Winston Oregon every storm and earthquakes happens the same day i was crucified0000 A.D. jews were forced to keep B.C. records along side A.D. tell my brother Jesus Nazareth came
@sourabhbabu2900
@sourabhbabu2900 Год назад
nice
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