A complete, step-by-step demonstration of the DNA Library Protocol, using the DNA Library prep kit (#56795) for CUT&RUN from Cell Signaling Technology.
• Visit the CST CUT&RUN Resource Center: cst-science.com/7sbx3x
• Follow along with written CUT&RUN protocols: cst-science.com/w6q77m
• DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795 (used in this video): cst-science.com/5sy20c
• Protocol video for the CST CUT&RUN kit: • CUT&RUN Protocol: A Vi...
• Overview animation: How CUT&RUN Profiles Chromatin • How CUT&RUN Profiles C...
This video demonstrates how to prepare a DNA library from CUT&RUN DNA obtained using the CUT&RUN protocol. Key protocol differences between CUT&RUN and ChIP-seq DNA library prep are highlighted.
Contents:
0:00 Introduction
3:20 I. End Preparation
5:05 II. Adaptor Ligation
6:57 III. Cleanup of Adaptor-ligated CUT&RUN DNA
9:45 IV. PCR Enrichment of Adaptor-ligated CUT&RUN DNA
11:24 V. Cleanup of PCR Amplification
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17 июл 2024
