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DNA sequencing methods 

Shomu's Biology
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DNA sequencing methods - this lecture explains Sangar sequencing method and Maxam Gilbert DNA sequencing method and next generation sequencing methods in brief.
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21 мар 2015

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Комментарии : 221   
@rrl9496
@rrl9496 3 года назад
I'm not sure why you go to such lengths to teach me these complicated topics for free, but I truly appreciate it...learned more in 15 minutes than I did in a semester of molecular biology!
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@asitdhir1204
@asitdhir1204 3 года назад
Where are you from @Macy sir
@Anlykabiofficial
@Anlykabiofficial 2 года назад
Free he is getting money from RU-vid
@Mettephobicemily
@Mettephobicemily 8 лет назад
It's amazing how a younger guy from around the world is teaching me stuff I couldn't learn from people that are right next to me. Thank you.
@brotherswinningbecauselove7857
Your trailer park neighbors probably know how to make bad ass bologna sandwiches though!
@akshitsoni6100
@akshitsoni6100 3 года назад
Keep up your work. I am an international student and a lot of my classmates are dependent on your videos for molecular genetics.
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts
@mahnoor4499
@mahnoor4499 3 года назад
Only shomu can teach such difficult topics in a very easy manner ...lots of likes from Pakistan 😍👌🤩🤩
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts
@bushrazafar7522
@bushrazafar7522 3 года назад
@@shomusbiologyofficial Thanks Sir for such great video we know every topic easily after watching your videos Sir you are very famous in our class lots of love from Pakistan 😊😊😊😊
@Ilemaurice687
@Ilemaurice687 2 года назад
@@shomusbiologyofficial hello plzz i really need that different methods used in the Analysis of DNA, including synthesis and sequencing
@sarahp.3163
@sarahp.3163 3 года назад
Could I ask you, out of pure curiosity, what is your level of study? (Masters, PhD...) . I have a Master's degree in a different, but related field and I couldn't imagine being able to not only having confidently absorbed all this amount of knowledge, but also being able to convey them in a clear and precise manner without hesitation
@sheeanagangadoo1852
@sheeanagangadoo1852 8 лет назад
Thank you, thank you, thank you... I have been looking for someone to explain to me all of the biological techniques for a long time and now finally found your channel and website - so grateful!
@shomusbiologyofficial
@shomusbiologyofficial 8 лет назад
Thank you very much.
@kashyapvekariya6887
@kashyapvekariya6887 5 лет назад
@@shomusbiologyofficial yeh
@snatachakraborty3124
@snatachakraborty3124 3 года назад
The 4 mins bridge amplification animation provided by Illumina was simplified into a less than a minute illustrateddemonstration by you! So clear
@Timabattah
@Timabattah 8 лет назад
Wow , just amazing, watching those videos reminds me of the true way and passion when one should teach biology. I haven t seen this while watching lecture in my college. Tahnk you so so much.
@alinasheikh7338
@alinasheikh7338 7 лет назад
thank u so much for being there during exams... it's very much beneficial for us
@dr.shipraagarwal2585
@dr.shipraagarwal2585 3 года назад
HELLO, I am pursuing MDS in periodontics, and i have to present one seminar on molecular diagnostic technique, so i didnt have a single clue about anything, because of your videos now i completely understood all the techniques. your videos are outstanding. superb. I am sure in future u gonna b someting great. good luck.
@SMAKA415
@SMAKA415 8 лет назад
Good job. I wanted a well overview only to review general sequencing methods and this was a good video to watch. Thanks.
@maansisrivastava541
@maansisrivastava541 3 года назад
I binge watch on shomus biology lectures than on any web series.
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Comment of the day
@Ammakodumai
@Ammakodumai 9 лет назад
i love allll your videos.. I am an Msc genetics and still cant understand some basic stuff but you explain very clearly. wish I could be your student..thank you soo much for this
@batkuparpohtam8070
@batkuparpohtam8070 4 года назад
Same here im msc student still culdnnt understand biotechnology properly.
@dr.pranjalikirde9665
@dr.pranjalikirde9665 5 лет назад
Ur just amaizng...i cnt tell hw much help m gettng frm ur vdos...thank u so much
@NurAlam-jd4yv
@NurAlam-jd4yv 8 лет назад
Wow.......... Carry on. Million of thanks for your great work.
@thanawatrattanathammethee9884
@thanawatrattanathammethee9884 8 лет назад
Billion of thanks guy !!You're awesome
@bikashdas1735
@bikashdas1735 5 лет назад
you are just understood me very clearly the fundamental of the DNA sequencing. As a msc biotechnology student this is very much helpful to me. Thank you so much and heats up to you.
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@sheikhnaseer4637
@sheikhnaseer4637 6 лет назад
one of the best and simple way of explaining........tnku sir....keep it up ...
@himanitrivedi1261
@himanitrivedi1261 7 лет назад
thanks a lot .... i waana to just quickly revision .. .. and its very useful.. keep it up
@vidhisambhvani8276
@vidhisambhvani8276 7 лет назад
oh man u r great...salute boss
@shama_parween
@shama_parween 3 года назад
if i am getting any problem in any sub or topic every topic is present on shomus biology in so easy way thank u so much 😊
@aparajitadutta1329
@aparajitadutta1329 6 лет назад
Love your lectures.. very informative!
@nourelhodaharidy2820
@nourelhodaharidy2820 8 лет назад
Thank you. It is very helpful overview.
@sreenivaspamisetty1757
@sreenivaspamisetty1757 3 года назад
Thank you ☺️ sir.......we are very blessed to have a wonderful teacher like you......🙏
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@Sunilkumar-oq7pr
@Sunilkumar-oq7pr 8 лет назад
Dear sir you are my faqvourite...and you are god for me.....great work sir i really vry vry glad
@lutfunchowdhury5711
@lutfunchowdhury5711 3 года назад
Thank you brother. It was convenient for very beginner's and too easy to run this information in my brain😇 Love from 🇧🇩 Keep growing, keep spreading knowledge we all wait and look forward for your videos.
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@paramveerpatil7451
@paramveerpatil7451 6 лет назад
Very good way of explaining.. opened up how I could think the ways now!! 😃😃
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+paramveer patil glad to hear that. Please subscribe and share
@T-Rexplorerr
@T-Rexplorerr 7 лет назад
YOUR VIDEOS ARE ULTIMATELY HELPFUL FOR ALL. THANK YOU SO MUCH .
@shomusbiologyofficial
@shomusbiologyofficial 7 лет назад
+priya dharishini thank you. Glad you liked my lectures
@sarichowdhary
@sarichowdhary 4 года назад
Thanks a lot. You are an amazing instructor, your learning videos really help a lot.
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Glad to hear that you're getting benefit from my lectures
@anitasingh7161
@anitasingh7161 6 лет назад
Thank u Sir its very useful lecture for my examination
@revanth3241
@revanth3241 Год назад
Literally saying, You are the RU-vid Radha Krishnan. Thank you for making Humans to Gain Knowledge.
@shomusbiologyofficial
@shomusbiologyofficial Год назад
I am honoured
@isissanjuan5027
@isissanjuan5027 4 года назад
I absolutely adore the way you share your knowledge
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Glad to hear that you're getting benefit from my lectures
@jaydeepdumadiya2464
@jaydeepdumadiya2464 3 года назад
I'm thankful sir.... God bless you...🙏🙏🙏🙏🙏🙏🙏🙏
@thatregulargirl9388
@thatregulargirl9388 2 года назад
Thank you Shomu bhaiya. You make studies easier and interesting at the same time.😇
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
You're welcome
@assistantprofessor8049
@assistantprofessor8049 7 лет назад
I like your video , its really helpful, please answer me how to add and how to select tag sequence for pcr primer
@senada5959
@senada5959 8 лет назад
A very informative video, very good
@manojkumar-vz4kw
@manojkumar-vz4kw 6 лет назад
Hello Shomu Biology. I am indeed very grateful the contents which you provide are excellent. Regarding sequencing I have a small doubt. Could you tell me exactly what is the possible role of dmso in normal pcr and also what is the role of betaine in sequencing pcr? I have been searching for this since very long time. It would be helpful for me if you can provide me with the answer. I hope my question is a valid one. Thank you in advance. Have a nice day ahead.
@nigeeacademy
@nigeeacademy 2 года назад
I appreciate you really, I used your video so many times, it is very smart and understandable.
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
Thank you so much for appreciating my efforts
@user-yn2vt6mi1s
@user-yn2vt6mi1s 8 лет назад
great work
@lanoanhduongthi3536
@lanoanhduongthi3536 8 лет назад
thank you sir good explain
@medicalangel2999
@medicalangel2999 9 лет назад
Thankkkk uuuu u r better thn my lecturer lol
@somosreeghosh8662
@somosreeghosh8662 6 лет назад
I will now be able to give my exam with ease...a big thanku..
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
Glad to help you.
@yashjain1296
@yashjain1296 8 лет назад
i want to know about sanger method . btw nice video. clear voice and explanation is super. (y)
@sagacioussadia9679
@sagacioussadia9679 6 лет назад
highly grateful to You Shomu 😃😃😃😃
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+Sagacious Sadia thank you. Glad you liked my lectures
@_atifch
@_atifch 4 года назад
nice sir keep it up i am impressed by you worldly knowledge.
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Thank you. Glad you liked my lectures
@AileenDaw
@AileenDaw 5 лет назад
Thank you! You are very didatic!
@jothipriyanka
@jothipriyanka 7 лет назад
Good work!
@Serwada237
@Serwada237 3 месяца назад
MSc molecular biology and biotechnology this is so good 🎉
@shomusbiologyofficial
@shomusbiologyofficial 3 месяца назад
All the best
@subhamoysharma6046
@subhamoysharma6046 3 года назад
Sir you always clear my concepts ❣
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you
@viniekouamou8188
@viniekouamou8188 7 лет назад
great video. keep it up
@skmasudulislam8200
@skmasudulislam8200 6 лет назад
Sir please discuss on topic CODING DNA
@sciencywiency
@sciencywiency 5 лет назад
Thanks Shomu, your videos are super helpful:D
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
You're welcome
@TruthfulPromise
@TruthfulPromise 3 года назад
Very Clear explanation. Thank you.
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome
@divyavarshney6222
@divyavarshney6222 3 года назад
Thank you sir. Sir which software can be used for the identify the orientation of DNA sequence....plz tell me
@AinaSakeenah
@AinaSakeenah 3 года назад
Thank you. Like usual, your videos always help :)
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@fatimahbrekdar6368
@fatimahbrekdar6368 7 лет назад
thank you very much . I just want to ask ,do we consider real time seq as a NGS ?
@zimamalamirew4808
@zimamalamirew4808 7 лет назад
great man, thank you to teach me heartily
@shomusbiologyofficial
@shomusbiologyofficial 7 лет назад
+Zimam Alamirew thank you. Glad it helped
@chefl2421
@chefl2421 5 лет назад
Thank you sir.
@toppe9349
@toppe9349 7 лет назад
Very much informative. Thank you.
@shomusbiologyofficial
@shomusbiologyofficial 7 лет назад
+abir shahnama you're welcome
@avdheshsharma6346
@avdheshsharma6346 6 лет назад
i'mma Fan i Rhyme like an insistent man you explaining Best everything you can then I'll combine the DNA Strand to build something that will be a gift to every Human Alive so that i'll increase minor bit of chances in this universe for us to Survive, Thanks for making these Videos Have a Happy Life.
@Aurochhunter
@Aurochhunter 4 месяца назад
I learnt this stuff years ago, it seems like ancient history now, but it's not as complicated as it seems if you actualy take the tein to learn it properly.
@shomusbiologyofficial
@shomusbiologyofficial 4 месяца назад
Thank you
@manjansinghkhadiya2739
@manjansinghkhadiya2739 6 лет назад
thank you sir
@adamprabowo4556
@adamprabowo4556 4 года назад
Maa shaa Allah, Thanks sir
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
@sriradhabhattacharya
@sriradhabhattacharya Год назад
Thank you so much for the videos. Can you please let me know whether DGGE is a classified as next generation sequencing? Thank you
@shirinbegum6464
@shirinbegum6464 6 лет назад
Please upload, protein sequencing method, my exams are near
@mayukhroy5393
@mayukhroy5393 4 года назад
This was helpfull Sir
@Doctor_Boy-01
@Doctor_Boy-01 8 лет назад
Thank you
@ritu5602
@ritu5602 3 года назад
Thnkuuu sir for teaching us in this way 😍😊
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you
@Hemlatasingh923
@Hemlatasingh923 9 лет назад
thank you
@hanaahezam9109
@hanaahezam9109 3 года назад
Really amazing video...thanx alot
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@hanaahezam9109
@hanaahezam9109 3 года назад
Veeeeery beneficent
@sabaakhtar4680
@sabaakhtar4680 5 лет назад
Kindly make a video on Helicos Sequencing
@obulamsnehith4423
@obulamsnehith4423 3 года назад
Ur are just amazing keep going on
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts
@sahed87
@sahed87 5 лет назад
Sir please make a video on glucose uptake bioassay and calcium uptake bioassay
@alankritarani1498
@alankritarani1498 7 лет назад
quite helpful
@Ilemaurice687
@Ilemaurice687 2 года назад
Sir plz tell me different methods used in the Analysis of DNA, including synthesis and sequencing
@shahid.baloch092
@shahid.baloch092 5 лет назад
Thank you very much, Dear. Wish you good luck for your success. :)
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@kuronotsubaki
@kuronotsubaki 9 лет назад
Hello, very helpful video, but I have some questions. I'm a newcomer and my english is still not very good, so please, be patient. Method bac to bac is based on DNA replication by using bacteria plasmids and further usage of the shotgun method with sequencing. Is it right? Can you tell anything about IonTorrent method? Is it NGS or SMS? What id the difference between NGS and SMS?
@leakurtek5098
@leakurtek5098 7 лет назад
please give the actual link to the videos you are refering to, it would be very helpful. otherwise, you rock
@sancharidatta1014
@sancharidatta1014 6 лет назад
Sir can u make a video about Edman degradation???if it will be possible...thn plzzzz upload it as soon as possible...
@Worldtravel4
@Worldtravel4 3 года назад
explain the 4 different sequencing techniques in detail. and show where the primers bind for each technique can you help me
@sivathesingam8945
@sivathesingam8945 2 года назад
No Words To Say Sir...♥️♥️♥️🦋💫
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
You're welcome. Glad you like it
@sivathesingam8945
@sivathesingam8945 2 года назад
@@shomusbiologyofficial 😇🦋♥️💫
@sivathesingam8945
@sivathesingam8945 2 года назад
Thank You Sir...🙏♥️🦋💫
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
You're welcome
@sivathesingam8945
@sivathesingam8945 2 года назад
@@shomusbiologyofficial 😇♥️💫
@ZemelakEndeshaw
@ZemelakEndeshaw Год назад
goooooood man
@alpanamishra6620
@alpanamishra6620 6 лет назад
I had query related to parameters of megablast please help as soon as possible
@aishwaryasewatkar5280
@aishwaryasewatkar5280 10 месяцев назад
Nice explanation 😊
@shomusbiologyofficial
@shomusbiologyofficial 10 месяцев назад
Thank you
@uzoechisamuel
@uzoechisamuel 3 года назад
Thanks
@kavis3554
@kavis3554 6 лет назад
very usesful this topic
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+kavi S thank you.
@deryacelik7743
@deryacelik7743 4 года назад
Hey it was great. But I didn't see Massive Parallel Sequencing? Cn y put this to get information pls ?
@lamzaasim1252
@lamzaasim1252 6 лет назад
I m in class12 I wanna know since all human are genetically different so how can we get a complete nucleotide sequence for a human genome .is it certain kind of average .could plz ans it early
@gopikhisan1874
@gopikhisan1874 6 лет назад
what is the direct and indirect methods for analysis of cloned genes?
@garyraab9132
@garyraab9132 4 года назад
Good overview. You must be gifted with a continuous fast talking stream gene..! Unfortunately I must have the slow hearing and processing gene! Be it true that I cannot improve my malfunctioning processing without resorting to CRISPR technology?.🙀.
@kiwivds1706
@kiwivds1706 6 лет назад
can you please tell me where you find the accurancy and price per million bases? I would like to use it for an assignment but I need a good reference
@abumonsurdinar
@abumonsurdinar 5 лет назад
I am in doubt about the pricing.
@naznintalukdar5191
@naznintalukdar5191 3 года назад
What is the cooling region nucleotide sequence ?
@samuelmwafulirwa6668
@samuelmwafulirwa6668 8 лет назад
thanks
@plantprotectionscience3646
@plantprotectionscience3646 7 лет назад
nice bro
@masufatima3775
@masufatima3775 3 года назад
Amazing 😍🙌 sir
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you
@christinajoycy5989
@christinajoycy5989 4 года назад
Is DNA sequencing method and genome sequencing both same?
@johirislam8174
@johirislam8174 2 года назад
According to Next generation sequencing which one is considered as first generation and which one is second generation sequencing???
@parkavik9697
@parkavik9697 3 года назад
Sir pls make a video about genetic code with detailed information...
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Okay
@ayeshamalik7526
@ayeshamalik7526 2 года назад
Any one can please share the link of capillary method sanger sequencing
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