Pyrosequencing 

Hi, I am a great fan of yours as you are doing an awesome job and your videos really help me a lot. But still I have to make this comment so that a misunderstanding does not take place. In the video at 8:28 you said by mistake that ATP converts luciferin into luciferase which is not true. Rather ATP converts luciferin into oxyluciferin that gives luminescence in presence of luciferase enzyme. I know you must have a clear idea about that and you probably had a slip of tongue (because later you only said from luciferin, light is produced with the help of luciferase). So, if you could kindly mention the correct information in a pinned comment or in the description then I think no further confusion will take place. Cheers! And keep teaching us!

By far the most simplest video of pyrosequencing I've possibly ever seen. You make us students understand such complicated topics with such accuracy and ease. Thankyou so much. Keep Uploading Content Sir. You are legit a blessing to the science community

When you said "if there is no thymine, then adenine will not attach and light will not be produced" I almost fell in love with you, you've saved my day. I am forever grateful. Keep doing your amazing work and congrats for the studying you have done in order to reach this level of understanding.

Watching this video 4 years later in 2020 is a bliss. Finally was able to grasp the idea of Pyrosequencing in probably the simplest way possible.

We are blessed to attend such amazing lectures and clarify our concepts 😃👍

Really useful and simple explanations. Thank you and please keep uploading content! Greetings from Costa Rica

I just got 30/30 votation in nanobiotechnology exam because of your videos...first person i tought was you...thank you my friend.

Thank you so much bro. I live in Korea and I am a high school student studying to get admission to Seoul Medical School. Your lectures and tutorials are so helpful bro. Take care man, take care.

I AM A DOCTOR ….I LOVE YOUR VDOS….KEEP GROWING……GOD BLESS YOU

well done May Allah ease every thing for you as you are doing for me

last day preparation for my exam >>> goddamn useful'

Sir ,when u say if u like the video then click the like option then i think that how anyone cannot like your teaching video. If i forget to click the like option then it does nt mean at all that i dont like. Thank u sir bundle of thanks sir.

U will win many hearts of students. Shomu bhaiya ur amazing. I hope ur channel grows a lot within this year.

APS is adenosine 5' phosphosulfate, not ammonium persulphate.

Thank you!!! You are the only one who was able to explain this to me!

Thank you sooo so so so so so so so much you really saved me .. thank u from the deep of my heart 🥺🥺🥺🌍🌍🇩🇿

Thank you so much sir for such concept clearing videos!! blessed to have this channel.

Thank you so much you saved my life

You tha man Shomu. Thanks for helping out.

Your videos make my study very easy👍 good job and more knowledge and wisdom to you

Thank you so much for your explanation it's really clear and it helps me a lot

Sir this type of video gives more understanding rather than animated one...

thanks for that wonderful explaination

Shomu da very informative video. There is one slight mistake : APS is adenosine 5' phosphosulphate. I have one doubt what is the use of adenosine alpha thiophosphate ? Btw great video Ur follower from KOLKATA ♥️♥️

Hi brother you are an angel tq for your dedicated work. You are blessed with the talent of making even difficult topics understand in a easy way and it is registered in our mind. I pray for your health wealth and happiness. Tq so much

these lectures never disappoint..🎉😊

I understanded alot of topics from your videos, big thank you Dr.Shomu's.

best and the easiest I've seen so far

Amazing explanation.. finally I get it!

You are a legend, explain everything so clearly! Thank you so much, you are saving me in my exam!

Appreciate much for the clear and interesting explanation for pyrosequencing.Its helps more on my understanding!

Really good teaching holding you have sir I can understand difficult concepts also thank you sir.

Brilliant ❤️

U r the best sir ur videos are just awsum😇😇

Thanku shomu sir for ur valuable and truly knowledgeble lectures I love ur all lecture series...and they are worth attending!!! GOD BLESS UH!! Always be happy and teach us as u r teaching..always😍😍🥰 so much thanku from my side

love you bhai ..u are really great

Thank you so much MR Shomu. i gain lots info..very clear and easy to understand..Done subscribed.

Wow u r amazing sir i love your vedios and how u explain u so better than our prof Thnk u so much God pless u

THANKYOU so much youhave helped me to understand prosequencing very well

Thankyou sitr💙

You are doing great job bro. APS is Adenosine phosphosulfurase

Thank you for the lecture🤯🤯👏👏

thank you very much, it is simple and perfect.

thanks you very much!!!! can u explain why do we need an adaptor please ?

This is very nice. Great! I wanted to request for targeted next gene sequencing. please make videos on this for better understanding, your explanation is too good. Thank you so much for making us to understand the pyrosequencing.

Thank you very much .It is very clear explanation.

Thankyou so much sir 🙏😊 great explanation

please make video on genome finishing - gaps and their resolution would be very helpful! ❤

Thank u soo much sir. It means a lot...

APS doesnt stands for ammonium per sulphate but it stands for adenosine phospho sulphate. still your lectures are really helpful

thanks sir, helps a lot

explanation is awesome

❤you are a true legend sir! 🙌❤

Thank you soo much sir for helping us out to clear our doubts.Sir in the video you have by mistakenly told the fullform of APS as Amonium persulfate but it should be( Adenosine 5' -phosphate sulfate )

Good explaination

@Shomu da, apni na thakle exam gulo te udhhar hote partam na kokhonoi! Happy Teacher's Day, Sir! Bhalo thakben! :)

very clear explanation. thank you very much

Thank you!❤

Very nice and well explained lecture

Thankyou! Can you make a video on solid sequencing as well? Thanks in advance :)

Recommmended to every interested Student. 😍😍😍

Our professor should learn from you

This is amazing!!! One question, how can we know if it’s a heterozygous?

much useful .. thankyou

Very well explained👌

very convoluted lecture

This is what learning should be like: fun

amazing you really did a great job......

Really love you Dada...

Very well explained. I have query : In a well we have multiple fragments (having varied sequence), then how can we detect the sequencing because at the same time nucleotides are added at the multiple fragments ?

Thanks, man 👍.

Welldone sir....

Very well explained ! Only one thing .... APS is Adenosine 5' phosphosulfate !

thank you very much, it is simple and perfect..

hello. really liked thie video. can you please make such a video on Support oligo ligation and detection(SoLiD) technique too? that will be great.

Hi sir am big fan of yours because of your outstanding teaching skills.... but one thing here I want to say that, I think here APS means ADENOSINE PHOSPHOSULPHATE not ammonium per sulphate.

Dear sir, thank very much for your nice and informative lectures, we have a request if you could provide a lecture about RAD sequencing. it will be your kindness.

this was helpfull Sir

Nice explanation sir!

Plz make video for solid sequncing

Thank you so much! I spent so much time trying to understand this from my lecture notes.

Thank you sir

Nice information

very well explained..... thanks mate

how does the light allow you to determine which base was added

You are the best thank you so much

Thank you Suman sir. It was nicely explained. In this regard I would like to clarify that here APS is Adenosine-5'-phosphosulfate, not ammonium persulfate. Hence it reacts with PPi to produce ATP. However your video is very helpful.

thanku sir🙏

Excellent video...helped me a lot

wonderful 🥰🥰🥰🥰 love u so much 😍😍

I really like it bro. make a video about shotgun and clone contig sequencing

At one time we add many nucleotides of same type or one only in the wells?

Thankyou sir

Hello, it's a very interesting video, I have some questions, How are the percentages of methylation obtained by the equipment generated? that is, how are they generated? How do you get that percentage? I have looked for this information but I have only found the basis of the chemical reaction but not of obtaining the percentage, I hope you can help me solve my questions, thank you very much

thank u so much

Thank you ❤

thank you

Nice sir

APS (adenosine 5′-phosphosulfate) is used. Ammonium persulfate (APS) is used in SDS Page.

APS in this case is Adenosine Phosphosulfate,Sir.