ELISA test procedure | what is elisa assay?

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Elisa test procedure - This lecture explains about the elisa test principle and the elisa test procedure. The elisa test is to identify specific antigen or antibody to detect the presence of bacterial infection. Elisa test can detect the presence of antigen or antibody in patient specimen that help us to detect bacterial infection. Stay tuned to know answers to the following questions -
What is elisa test?
Elisa test procedure?
Elisa test principle?
Elisa test classification?
How elisa test is done?
What is elisa assay?
Elisa test is also known as the enzyme linked immunosorbent assay.
Stay tuned to know the elisa test explained in this video lecture.
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Thank you for watching the video lecture on elisa test procedure and the principle of elisa test.

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29 сен 2024

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Комментарии : 216

@zakiyasohana3037 9 месяцев назад

Legends are watching this video one day before exam 😅 btw....this video is very informative easy to understand helped me a lot. Thanks Shomu's biology

@shomusbiologyofficial 9 месяцев назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@simadassaikia8588 5 месяцев назад

Mera kal exam hain😂

@zakiyasohana3037 5 месяцев назад

@@simadassaikia8588 okay carry on 😄 I think you're from Assam 🤔

@simadassaikia8588 5 месяцев назад

@@zakiyasohana3037 Yes I am form Assam

@zakiyasohana3037 5 месяцев назад

@@simadassaikia8588 From which institute?

@27dodiyadhrupika Год назад

👌👌👌👌👌

@shomusbiologyofficial Год назад

Thank you

@deepapramod4472 3 года назад

👍👍

@shomusbiologyofficial 3 года назад

Thank you

@Taenowritesfictions Год назад

Thankyou so much sir... Tomorrow is my exam and I wasn't able to understand a single thing about this topic but you made it easier. Thankyou so much again. Your new subscriber 🌼🌼

@shomusbiologyofficial Год назад

You're welcome. Glad you liked it

@Taenowritesfictions Год назад

@@shomusbiologyofficial sir today I was able to write on it perfectly.Thankyou again sir. This video helped a lot and I recommended this to my friends too.

@b.a.i.s.h.y.a 11 месяцев назад

Same case with me

@yasminemahmoudrefaat7042 2 месяца назад

me too

@ebenezerandoh488 2 года назад

Hello! we're really thankful to you for all your brilliant videos about life science. you're just giving us the breakthrough into life science for free, we do appreciate that and will also support you to grow. Again, your explanation is so vivid, real in the sense that even a layman person could easily comprehend unlike others who will be just reading a downloaded ppt from google. continue.. your good work and may God be with you.

@kalaiselvi6151 3 года назад

If primary antibody has the affinity to bind to the well, then antigen vil also have the affinity to bind to the well n there is a chance of binding of secondary antibody to the antigen n gives us a colour in the end.

@gururajkalshetti198 2 года назад

Primary antibody don't have with Affinity to bind well but it is fixed in well

@padmajaofficial5863 2 года назад

Elisa plate or well is coated with blocking agent that prevents further binding or unnecessary binding

@nilutpaldas18 Год назад

You r the warrior, our saviour. I become familiar with a topic of which I don't even know a single word and able to write it completely in exam. Thank you my teacher. ❤❤

@shomusbiologyofficial Год назад

Glad you liked it

@kenrashi 3 года назад

Thank you sir. for making my ongoing MSC. So easy for me _💕

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@akankshabhatt3357 2 года назад

Thankyou so much for your videos, you've helped me study for the MCAT (medical entrance exam in the US). I genuinely have learned so much from your videos and no matter wherever I am meant to head into the future based on my results, your videos always inspire me to keep on learning and expand my horizon and breadth of knowledge. God bless:)

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts. I am Honored. Stay tuned for more help. All the best for future career

@debleenabhattacharjee6709 Месяц назад

THANKYOU SO MUCH you made it COMPACT and very EASY thankyou sir

@shomusbiologyofficial Месяц назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@kamrunandalfaz Год назад

Sir i can't find elisa or ria incase of hormone assay which is in my syllabus...one day left for my exam...please reply here that incase of hormone how can l use the word hormone in substitute for antigen antibody... Please sir give me reply... Nobody can tell me this answer... I am waiting sir🙏🏻🙏🏻🙏🏻🙏🏻🙏🏻

@shomusbiologyofficial Год назад

I am glad

@sabitasinku9548 2 года назад

Maine +3 apke video dekh kar khattam ki hai ,msc first year main hoon aur mujhe ye bhi pata hai k app ke vdo hinn mujhe pass kar sakti hai ,thank you sir 😁😁😁you are excellent 👍🏻👍🏻👍🏻👍🏻 learning from odisha

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@beenzumulalu1738 29 дней назад

This video is very helpful. Thank you @Shomu's biology 👏🏽

@shomusbiologyofficial 29 дней назад

You're welcome

@kamalakantakapat6768 8 месяцев назад

Now i have a confidence that i will be pass....Thank you sir 🙏

@shomusbiologyofficial 8 месяцев назад

All the best for future career

@zahidaessa2336 2 года назад

Sir kindly explain application.

@mubarrahamjad3246 2 года назад

Very well explained! I have seen many videos on elisa before but you explained in best way .. and finall I understood the concept of this topic. Thanx alot

@shomusbiologyofficial 2 года назад

You're welcome. Glad you like it when

@AnjaliSingh-mn5qc 2 года назад

Thankyou sir. I am ug student of Biotechnology and am doing my internship in medical College and continuing this topic. You explained very well sir I have understood the whole only in one hour. Thankyou plz keep up posting.

@shomusbiologyofficial 2 года назад

You're welcome. Glad you liked it

@satyajyotikanjilal491 Год назад

Thank you. Very nicely explained the complicated technique in such a simple way. Best Regards

@shomusbiologyofficial Год назад

Thank you so much for appreciating my efforts

@SG-ot4uc Год назад

You surpassed uni doctors by ur amazing explanation. Thank so much for ur efforts. Can you please download more videos for data analysis please 🙏🏻

@shomusbiologyofficial Год назад

Thank you

@BiobyNishit Год назад

sir please explain easy methods first and then go for complex methods,

@shomusbiologyofficial Год назад

All methods are already explained

@Sharvarthani 2 года назад

Such a wonderful explanation sir Thank you very much Sir ✨✨ Favourite teacher ever

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@poojakumaran6734 Год назад

My favourite channel where topic is explained perfectly in a simplified way...

@shomusbiologyofficial Год назад

Thank you so much for appreciating my efforts

@Rohitjhilikshorts 3 месяца назад

This video is very informative nd easy to understand

@shomusbiologyofficial 3 месяца назад

Thank you so much for appreciating my efforts

@Quesofoods 3 года назад

You are educating us with a best way thankyou so much sir👌👌

@chaibiscooot6715 3 года назад

Hello. Can you please do a video or notes on how to calculate the reagents of indirect ELISA. Please!

@shomusbiologyofficial 3 года назад

Okay I will try

@Wiltflower 5 месяцев назад

during washing the unbound substance is remove from the whells😊

@kulsumnishat1620 3 года назад

Sir you are a fabulous teacher.. Sir Please make a video on enzyme technologies too.

@shomusbiologyofficial 3 года назад

Sure. Thank you

@sowndaryana197 3 дня назад

Thank you sir 🌸

@shomusbiologyofficial 3 дня назад

You're welcome

@roshnibhatt2801 Год назад

Thankyou so much sir, I got very clear cut differences of all types of ELISA Test, very well explaination

@shomusbiologyofficial Год назад

Thank you

@Ravifddccvf Год назад

If you use wrong buffer or simple water than also kit will show accurate results

@ishfaqahmad9690 2 года назад

The Direct Elisa and Sandwich Elisa is same...Correct it

@aamaramuzaffar3565 3 месяца назад

Very helpful 😍 amazing

@shomusbiologyofficial 3 месяца назад

Thank you

@syeedayousufy Год назад

I have wached many videos but i have not got the concept, like always you make my doubts crystal clear thak you so much Sir.

@shomusbiologyofficial Год назад

Glad to hear that you're getting benefit from my lectures

@kalyanlohar476 2 года назад

Thank you sir... Very nice informative lecture sir. and very excellent lecture sir... Thank you 💞💞💞👍🙂

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@shreyanagpal1693 Год назад

You are indeed the best teacher!!!!!!!

@shomusbiologyofficial Год назад

Thank you

@rejoanakarimriana914 3 года назад

I m an undergraduate student of Biochemistry and of course so thankful to you for making our courses so easy❤ Eager to know more about your degrees and qualifications. Please share some with us so that we get motivated more. . Salam from Bangladesh ❤

@shomusbiologyofficial 3 года назад

Glad to hear that you're getting benefit from my lectures

@rejoanakarimriana914 3 года назад

You are absolutely great.. Best wishes ❤

@ridamasood9878 2 года назад

Much respect from pakistan Sir.

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@pratanupatra4006 2 года назад

Thank You Sir So Much For The Video......You Explain So Well I am a BMLT 3RD year Student and Your Videos Helped Me ALot ....Keep Sharing More Videos 🤘🏻🙏🏻🥰❤️

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@vlogtomywelcome7979 Год назад

I can clear my masters because of these types of videos by you sir.. thank you so much

@shomusbiologyofficial Год назад

You're welcome

@subhendunayak Год назад

Thanks 🙏

@shomusbiologyofficial Год назад

You're welcome

@subhendunayak Год назад

@@shomusbiologyofficial i want lecture notes of this topic

@alinasayyed9593 11 месяцев назад

Thankyou so much... watching it 3 hours before paper/

@shomusbiologyofficial 11 месяцев назад

You're welcome

@divashah7920 Год назад

very very well explained sir thank u so much sir 👌👌🙏🙏🙏

@shomusbiologyofficial Год назад

You're welcome

@fullmoon4831 Год назад

❤❤❤❤

@harriniharri1687 Год назад

This Topic became so clear because of your lecture🙏🏻🙏🏻 Thank u so much sir ☺️

@shomusbiologyofficial Год назад

You're welcome

@StellaNoire 2 года назад

Thank you so much Shomu Ji- you are such a blessing!

@shomusbiologyofficial 2 года назад

You're welcome

@nehanegi6345 5 месяцев назад

In plants ELISA is different?

@Raj_02_07 8 месяцев назад

Thank you so much sir 🙏

@shomusbiologyofficial 8 месяцев назад

You're welcome

@eleven9701 Год назад

Can someone tell me what peroxide is it ?

@prajiktalonkhade1258 Год назад

Thank you so much sir

@shomusbiologyofficial Год назад

You're welcome

@sukanyarakshit9662 Месяц назад

Must watch

@shomusbiologyofficial Месяц назад

Thank you

@sangeethabaskar9560 Год назад

Best vedio ever 💥💥💥💥

@shomusbiologyofficial Год назад

Thank you

@FatimaSaadvlog 2 года назад

I requested you k mery exams hyn plz made video on types of biosensors and you highlighted my comment but don't made.... disappointed

@shomusbiologyofficial 2 года назад

It's not that simple. I have videos lined up.

@sanjaykumar-ew6mb Год назад

Please make video on ABO DESCREPANCY IN DETAIL IN HINDI PLEASE DEAR SIR ❤❤

@shomusbiologyofficial Год назад

Okay

@dhanrajpaulp6678 2 года назад

Hi shomu.hi shomu ❤️

@rameshsbiologyacademy1042 Год назад

Hi sir. Gd morning! Is secondary antibody which has linked with enzyme is similar to the primary antibody?

@shomusbiologyofficial Год назад

No. Secondary antibody binds to primary antibody and it has enzyme linked to it

@iqbalsteaching2272 Год назад

Amazing teaching sir! Sir can u tell that ELISA is specific to only proteins test or it can also be used to detect any microorganism? Im not associated with this just asking for gk!

@madpools_life Год назад

Why only detects protein ?

@pieroguastella9639 2 года назад

Thank you so much for your videos. I have just passed my biochemistry exam using your videos and i got top marks. You are the Best ❤️

@shomusbiologyofficial 2 года назад

You're welcome

@Michela-ng4is 2 года назад

Grandeee

@xaidytff4487 2 года назад

Thank you sir, it was so clear and understandable..❤❤❤❤

@shomusbiologyofficial 2 года назад

You're welcome

@JayAcharya-fz4kl Год назад

Sir ,I hardly request you to make one video on Immunological assay .

@shomusbiologyofficial Год назад

@sasisasi-gn7xk 3 года назад

Sir ..,we want optical immuno assay vedio

@shomusbiologyofficial 3 года назад

Okay

@risharajkhowa5060 Год назад

Sir, your way of teaching is just awesome 🙏

@shomusbiologyofficial Год назад

Thank you

@drvarshayadav5511 Год назад

sir wanted to know if the intensity of colour formed has anything to do with intensity of the disease?

@niravpatel8674 3 года назад

Sir... basic peptide of tissue .... in thic topic no any one video in youtube....so please made it

@tts9086 2 года назад

Sar Video Hindi banaa dijiye

@gshanmugam5552 Год назад

Why serum is preferred than blood in ELISA test

@haroprasadpaul 2 года назад

Thank you sir

@shomusbiologyofficial 2 года назад

You're welcome

@md.shirajurrahman9082 2 года назад

Great job done... Keep moving forward...

@shomusbiologyofficial 2 года назад

Thank you

@rezoanulfahim8237 10 месяцев назад

much of love for you my dear brother

@shomusbiologyofficial 10 месяцев назад

Thank you

@sanjaykumar-ew6mb Год назад

❤❤

@shomusbiologyofficial Год назад

Thank you

@sampathkumar-wx9oo 2 года назад

Superb explanation sir....👏

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@dr.mohddanishstudyzaheer2558 3 месяца назад

Your qualifications my dear

@shomusbiologyofficial 3 месяца назад

Msc in Microbiology

@sijinmathew6299 3 года назад

Omg is this video for me👀Tomorrow i have immunology Exam🙂🌚... Plz Bless be My sir@shomus biology

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@sijinmathew6299 3 года назад

@@shomusbiologyofficial it's my pleasure sir... Really you doing great work♥️😍♥️

@nigamvyas4272 3 года назад

Can I write this on exam paper??? As it is....?

@iqrahassan3955 2 года назад

Thank you sir you are amazing 🤩

@shomusbiologyofficial 2 года назад

You're welcome

@meghna406 2 года назад

Thank you so much sir ☺️

@shomusbiologyofficial 2 года назад

You're welcome

@swathys650 2 года назад

Excellent video for elisa

@shomusbiologyofficial 2 года назад

Thank you

@harishdr885 2 года назад

Sir thank you so much

@shomusbiologyofficial 2 года назад

You're welcome

@KalyanisVegPlanet 3 года назад

PLEASE DO VIDEO ON GMO Testing, and why it important to test GMO in food sample

@shomusbiologyofficial 3 года назад

Okay

@esraasalim4646 2 года назад

What about capture Antibody ?

@zainabsiddique2222 Год назад

In the case of sandwich ELISA, we have a secondary antibody that can interact with a particular region of primary antibody then why after second washing and adding substrate it will not show color?

@kamrunandalfaz Год назад

Cause the substrate not individually added it is linked to the sec antibody

@nimramumtaz07 Год назад

Excellently explained ✨

@shomusbiologyofficial Год назад

Thank you

@Shirmila224 3 года назад

Can U explain Geneious De novo assembly and Contig mapping ??

@amnariaz6790 2 года назад

Does serum contain antigens!??

@Sweetweaponn 3 года назад

Sir please do make a video on how to prepare for GATE biotechnology 2021🙏🙏🙏

@shomusbiologyofficial 3 года назад

Okay

@dayanimitesh4786 2 года назад

Sir as you had told antibody- antibody don't bind to each other on time 12:56 in video. but in previous video of yours for topic Western blotting you told that antibody binds to antibody Fc region and shows color in solution.

@gururajkalshetti198 2 года назад

But the Fc region is already attached to well or may be both antibodies are not similar to bind each other ... As per my understanding i think that might be the reason ab doesn't bind to ab

@abhinavjain5076 3 года назад

Very well explained. I have one question why we skip washing step in direct Elisa because we are using enzyme linked antibody and in the next step have to add substrate , substrate because product by enzyme or if antibody is not linked to particular analyte but we have enzyme linked antibody and substrate in solution so is there any chance of colour production. I am so confused please explain

@shomusbiologyofficial 3 года назад

Watch the other varieties of elisa lectures

@mtk8771 3 года назад

Hi dear You didn't repeat your antibodies test after completion of two weeks to got vacinated Please repeat

@shomusbiologyofficial 3 года назад

Soon

@vishnukumart3371 3 года назад

Such a Lovely lecture 🤗

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@manaswinibal6594 2 года назад

Thank you sir

@shomusbiologyofficial 2 года назад

You're welcome

@poonamkoirala4466 3 года назад

Sir please make a video on ESBL and MBL

@tekalignzeleke7331 2 года назад

Where is the enzyme to add?

@lakshayasethi4013 3 года назад

Why will secondary antibody bind with antigen x when ag and ab follow lock and key mechanism ? when it has already bound with primary ab it shouldn’t bind with the other ab.

@aqsanaz1783 2 года назад

well, your question is very good but my point of view the answer is that although Ag Ab bind together so secondary Ab also follow the same lock and key model it does not follow any other method to bind with Ag, lock and key model does not say that one Ag Ab phenomenon always bind, does not matter how many Ag or Ab bind. Hope u got it

@vennila8787 3 года назад

Sir how to design the primary ab against ag x ?

@_silent_eyess 2 года назад

Hello sir can u pls create another video on flow cytometry ....this video is very helpful for me and for many students ...you are truly doing great job for students who have lots of doubts and with your educational video all doubts easily cleared

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts. Coming soon

@syedhaya5723 2 года назад

What an explanation !!! 🔥🔥🔥

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@syedhaya5723 2 года назад

@@shomusbiologyofficial I was wandering here and there to make myself understand the actual concept, went through a lot of videos but this one cleared my concept,, keep spreading quality knowledge 🤍🤍