PUMA is a DIY open source portable 3D printed microscope with augmented reality, fluorescence, polarisation, dark ground, phase contrast, epi-illumination and other advanced features. Its illumination system (called 'Dominus') has many configurations and in this video I show you how to build and use the coaxial epi-illuminator for epi-fluorescence and reflected light microscopy including metallurgical analysis. I also demonstrate these features with many images taken with PUMA of a fluorescent test slide and a silicon chip die imaged up to the limit of standard optical microscopy resolution with oil immersion. I also show a comparison to a professional epi-illumination microscope - the Olympus BH2 BHT-ML metallurgical microscope.
CONTENTS
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00:08 Overview of epi-illumination microscopy
01:34 The PUMA epi-illumination system
06:35 Black holes and the Event Horizon
13:13 Types of photomicrography used in this video
15:26 Epi-fluorescence example: plant histology
19:03 Reflected light low power: PCB
20:53 Afocal photography: Silicon chip die with Olympus BH2 comparisons
22:24 Direct chip projection: Silicon chip die (PUMA only)
26:58 Building the Epi-illuminator
30:18 The clip lock and stay thumb wheel
34:10 Concluding remarks
SUPPORT PUMA
============
PUMA is an open source microscopy project. You can help support the project by:
1. Become a Patron: / pumamicroscope
2. Donate via PayPal: www.paypal.com...
3. Simply subscribing to this RU-vid channel, like, comment and share these videos.
4. Spread the word - post the news about PUMA and link to this RU-vid channel on your social media sites and any other outreach method (tell your friends, colleagues, students or teachers and lecturers about PUMA, for example).
5. Consider purchasing your optics and related supplies from our affiliated online optics store, OptArc.co.uk ( www.optarc.co.uk/ )
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PARTS AND TOOLS
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For more details see the 'Bill_of_Materials' on the PUMA GitHub page ( github.com/Tad... ) which also gives examples of where you can buy some of the more specialised items.
Models revised since the v1.0 release of PUMA:
Monocular
- Occular_Extn_CM - New model ocular tube for direct C-mount connector
- Monocular_tube_CM - New model direct C-mount connector.
Filterblock
- Side_Port_Separate_EpiStop - New model optional AR side port with baffles.
Models covered in previous videos (not repeated here):
LED and Lower Collector
• The LED Illuminator an...
Trans-Polarisation Illuminator
• The Trans-Polarisation...
Köhler Illuminator
• Köhler Illumination - ...
Condenser Upgrade
• The Abbe Condenser wit...
Beam Splitters and Advanced Filter Block
• Beam Splitters and the...
3D Printed Models (quantity)
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DI_Cnd_Adj_thumbwheel.stl (4)
DI_Cnd_gripper.stl (1)
DI_M3_Adjustment_ring.stl (1)
DI_Epi_attachment.stl (1)
DI_Epi_black_body.stl (1)
DI_Epi_condenser.stl (1)
DI_Epi_Cnd_Aperture_04.stl (1)
DI_Epi_Cnd_Aperture_10.stl (1)
DI_Epi_Cnd_Aperture_13.stl (1)
DI_LC_Adjust_collar.stl (1)
FB_Side_Port_Separate_EpiStop (1)
DI_Epi_pol.stl (1)
AR_Clip_lock.stl (1)
AR_Clip_c_adhesin.stl (1)
AR_Stay_thumbwheel.stl (1)
FB_Filter_F17_slider.stl (1) - for fluorescence only
FB_Filter_slider.stl (1) - for analyser
FB_Filter_collar.stl (1) - for analyser
Parts for the advanced filter block
Additional filter sliders and filter collars as required.
Protective caps as required.
Illuminator parts as required.
Non-Printed Parts and Tools
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M3_Hex_Head_Set_Screw_10mm_long (1 for each M3 thumbscrew)
M3_Full_Nut (1 for each M3 thumbscrew, 3 for each illuminator adjustment ring and 1 for the clip lock)
M4 Screw 14 mm long (1 - for the front of the focus plate to take the stay thumb wheel)
M4_Full_Nut (1)
M2_Allen_Bolt_Socket_Cap_DIN912_12mm_long (4) for epi-polariser
For fluorescein fluorescence:
Filter fluo yellow (510nm long pass 17 mm diameter 1.5 mm thick)
Filter fluo blue (450nm narrow band pass 14 mm diameter x 1 mm thick)
Allen keys (for the screws)
Tools for cleaning up 3D printed parts (e.g. craft knife, wire brush)
FOR FURTHER INFORMATION
=======================
1. Open access write-up about PUMA on the FreeCAD blog:
blog.freecad.o...
2. The official PUMA GitHub page where you can download the specs and source files to build or customise your own PUMA system:
github.com/Tad...
3. The scientific peer reviewed publication on PUMA that was published in 2021 in the Journal of Microscopy (a journal of the Royal Microscopical Society) available here:
pubmed.ncbi.nl...
onlinelibrary....
Thanks for your interest in the PUMA microscope system.
PJT 14/02/22
29 авг 2024