One of my favorite AK lecture quotes "And what that means is...." Everything is always explain so thoroughly. Una de las citas que me gusta más es cuando él dice " Y lo que eso significa es.." Todo se explica con tanto claridad, lectores tan buenos.
you are a masterpiece man!!!! My favourite line is, " ........as we shall see in just a moment." Continue simplifying life during my studies. Thanx a lot, that was really helpful
Hey, I find your notes super helpful. I wish there was a way I could look back at them without watching the video again. Is there a way you can upload your notes on the board somehow?
Love your videos! I took molecular biology in college and I did not understand this particular pathway but it is becoming clearer now. just one thing though, GDP becomes GTP when epinephrine binds to 7tm, where does the phosphorus come from to bind to GDP? Is it just randomly "wandering" out in the cytoplasm and just happens to bind to the GDP making it GTP?
Isaac Murphy Awesome :) Yes, the GTP is randomly moving about the cytoplasm. Upon binding to the receptor, the epinephrine causes a conformational change that constricts the space that GDP is in and that squeezes it out. At the same time, it creates a space that can fit in the GTP, so as the GTP randomly collides with it, it feels a strong enough electromagnetic force that brings it in. So we exchange one guanyl nucleotide for another (We don't simply add a phosphoryl group).
It’s true that upon binding with GATP the alpha unit of G associated protein binds with Adenylate cyclase but the books state that the movement starts with displacement of adenylate cyclase but I get the impression that the alpha unit initiates the movement. Kindly clarify..
A single epinephrine can cause many G proteins to exchange GTP for GDP. This is not simultaneously is it? I imagine the signal molecule can unbind and go on to do the same thing on other receptors. Is this right?
