Estimation of Blood Urea By DiacetylMonoxime (DAM) Method || Serum Urea Estimation

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Estimation of Blood Urea By DiacetylMonoxime (DAM) Method - This is the video on estimation of Blood Urea By DiacetylMonoxime (DAM) Method, Calculation of Blood Urea Nitrogen (BUN), discussion of various viva questions with demonstration on estimation of serum urea on colorimeter.
Methods for estimation of blood urea - 00:54 minutes
Principle of DiacetylMonoxime (DAM) Method - 01:29 minutes
Reagents for urea estimation - 02:03 minutes
Procedure for urea estimation - 02:34 minutes
Reference range of blood urea - 04:11 minutes
Blood urea nitrogen (BUN) - 04:24 minutes
Calculation of Blood urea nitrogen (BUN) from Blood Urea - 04:50 minutes
Interpretation of Blood urea/ Causes of Increased Blood Urea - 05:34 minutes
Urea Clearance Test - 07:09 minutes
Azotemia and Uremia - 07:48 minutes
Discussion of Viva questions - 08:21 minutes
Demonstration of Estimation of Blood Urea By Diacetyl-Monoxime (DAM) Method - 09:14 minutes
#Serumureaestimation #bloodureaestimation #DiacetylMonoxime (DAM) #Biochemistrypractical #Biochemistryvideos #renalfunctiontest #dmltpractical

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21 май 2020

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Комментарии : 57

@amitabenpatel93 4 года назад

Sir plz tell 1)why blood urea nitrogen Is a best indicator in USA than urea? 2)which is the best mathod among 3 those you have mentioned in this video? 3)and what difference can happen in blood urea and serum urea value?;

@Biochemistrybasics 4 года назад

1. Chemical formula of urea is ( NH2 )2 >C=O i.e. Molecular weight of urea is 60 while nitrogen in it is 28 . 60/28 = 2.14 , so the conversion is blood urea nitrogen × 2.14 = blood urea , a calculative value . All the automatic instruments used in laboratory measure BUN which is converted to the urea by multiplying 2.14. Plus other substances like NH3 can interfere with urea estimation so its better to give value in BUN. 2. Urease is best for the estimation of urea as its kinetic method. 3. Blood urea is the misnomer term which is used from the very beginning. Actually we are estimating urea in Serum/plasma. So no such difference between Blood urea and Serum urea. Hope it helps.

@amitabenpatel93 4 года назад

@@Biochemistrybasics thank u Sir

@Biochemistrybasics 4 года назад

🙏😊

@ayushiabhipsa7846 Год назад

Thank you so much sir for uploading this🙏🏻🙏🏻Its so so helpful for last minute exam revisions and hats off to your explanations 😊🙏🏻

@Biochemistrybasics Год назад

Glad you found it useful

@tsanjeshiu5176 2 года назад

Such a great video 👏 Thanks a lot

@Biochemistrybasics 2 года назад

My pleasure dear

@vineets.h8784 2 года назад

Lucidly explained sir thank you

@Biochemistrybasics 2 года назад

My pleasure dear

@mehulpanchal8127 4 года назад

It is good video and learn more about serum Urea. and technical knowledge for MLT students...

@Biochemistrybasics 4 года назад

😊🙏. Spread the word.

@madhuribhatt3068 4 года назад

best video sir,all doubt solve ...seen the video ,lots of thank you🙏☺

@Biochemistrybasics 4 года назад

🙏😊

@muneebbashir5027 4 года назад

Thankyou very much sir

@Biochemistrybasics 4 года назад

🙏😊

@amtkhandelwal 4 года назад

Love to listen you bro... ♥️

@Biochemistrybasics 4 года назад

🙏

@PremaSiriwardana-te4tq 28 дней назад

Great sir❤

@Biochemistrybasics 28 дней назад

@@PremaSiriwardana-te4tq thank you

@vaddesaikumar4690 3 года назад

Thank u sir thank you so much❤❤😊😊❤❤❤❤❤

@Biochemistrybasics 3 года назад

My pleasure ❤️😊

@vikramajgar9787 2 года назад

Sir ur awesome ....💐❣️

@Biochemistrybasics 2 года назад

You are super awesome 😊

@aartijha7049 Год назад

How to do calculation of same by graph mathod?

@akshayjouhary3897 Год назад

Good job

@Biochemistrybasics Год назад

Thanks a lot

@bablurockstar52 Месяц назад

Sir I am Anjali DMLT it's Azotemia is it's DMLT class or not

@NgocAnhNguyen-tm5hz 3 года назад

Wow

@koushikimekala5705 3 года назад

Sit what is the importance of 'blank ' and 'standard' in quantitative analysis.

@user-jr8yw2ks9p 3 месяца назад

Blank (B) is used for caliberation Value will come lower than other S and T Colour is lighter Standard (S) - used for identification Dark in colour compared to B Test (T) - used for finding unknown conc. Among these three T is darkest in colour

@ayeshajahan1291 4 года назад

Keep making such videos

@Biochemistrybasics 4 года назад

Definitely

@swarnavabiswas3827 3 года назад

How I will get this standard and Test solution for My laboratory. Please help me

@Biochemistrybasics 3 года назад

For preparing Urea Standard of 30 mg% dilute 30 mg of urea in 100 ml DW Same way for 25 mg%

@swarnavabiswas3827 3 года назад

@@Biochemistrybasics Thanks a lot Sir

@gulfwarveteran8528 4 года назад

👍

@jaanjabeen2636 2 года назад

@Biochemistrybasics 2 года назад

Most welcome dear

@poornakumari3394 Год назад

Plz video upload on estimation of blood glucose by folin Wu method

@Biochemistrybasics Год назад

Okay

@arati234 3 года назад

Normal urea level in serum and normal level of urea in urine . please say sir

@Biochemistrybasics 3 года назад

Normal urea level in urine is 8-20 mg/dl while normal blood urea is 15-45 mg/dl

@debanjanbhattacharyya2110 Год назад

Is urea reagent ready-made solution available commercially? Or you have made it conventionally?

@Biochemistrybasics Год назад

Ready made available

@debanjanbhattacharyya2110 Год назад

@@Biochemistrybasics Thank u very much...Sir.

@diljithkk1 4 года назад

Estimation of sgot and sgpt🙏🙏please

@Biochemistrybasics 4 года назад

Yes. Will upload it. 🙏😊

@cristina4452 3 года назад

Hello.My urea is 19...is that ok,?

@Biochemistrybasics 3 года назад

Yes it's normal

@ayeshajahan1291 4 года назад

Great work. But I'd like to point out that After decimal we don't say the figure, we say the digits. E.g. 2.14 is not two point fourteen. It is two point one four.

@Biochemistrybasics 4 года назад

Yes. 🙏

@ayeshajahan1291 4 года назад

@@Biochemistrybasics glad to help