This video is exactly what I needed, I'm piecing together a home lab and have tons of questions and this video helped with so many of them! Thank you!!!!
Hey Tony, I have been watching your videos a lot, it inspired me to start growing mushrooms for the kitchen at home. You are awesome at making things clear and easy to follow. Thank you.
You can get reusable glass Petri dishes and lids. The parafilm won't leave an adhesive residue and is easier to mold to shape. Have you tried using a bacterial inoculation loop tool for the samples to be cloned, instead of the scalpel? Thin wire loops and easy to get red hot in flame. Bunsen Burners?
Great primer! Im wanting to get started growing lions mane. I've got a small location purchased but it needs remodeling before i can get started.. I think i probably ought to practice at home while i remod the building. More videos like this are exactly what I need as I begin...
What makes you say the presto will need replacing? As a chef, I've several old pressure cookers, including an older model of the presto one - if you aren't abusing them, the only thing that should ever need replacing is the seal, and sometimes but rarely the safety pressure release valve. There isn't really even anything else to wear out...
As someone who cans and does tattoos( an autoclave is absolutely mandatory in tattooistry) both types of pressure vessel have a limited valve life and perform the same task. We really only need the medical grade one because of its size and ease of use; besides that they both provide ample pressure and life-time-use.
@@justinlangley9529 yeah... I did acknowledge that "the only thing that should need replacing is the seal" - ie: the gasket IS the seal when a gasket is used.
Hey mentioned it in a reply to someone on here. He said that the they are both good and that you could replace the seal on the presto and that the only reason he had to replace / upgrade his was because he let it run out of water a few times and eventually the 2 he did have warped over time due to letting it run out of water on accident.
Thanks for the informative video, i did not have interest in mushroom farming at all, but aim starting to like mushroom farming since it seems to be profitable when your doing it well. Aim working with one of the biggest mushroom farm in Israel of marina which is really beautiful farm but very automated. when i see the operation process that are going on with are very advanced technology aim even giving up to start a mushroom business farm. but since i started following your videos i feel like its possible to start it but the issue is what a starting capital should you have though i understand always varies according to the location. but i think you need to make some video that can show , the simple cost outlay for small scale farmers and also the various building options that we can opt for. once again thanks for your great ideas aim really impressed
Hey Tony! Thanks for all of the information and for putting these videos together. Where did you get that little metal stand that you use in front of your flow hood?
Your videos are so incredibly helpful! You're articulate and get to the point quickly, which I appreciate as an educator myself haha. I'm in the process of slowly getting the things I need for my new hobby but one thing I'm really struggling with is the use of so many single-use plastics. Would you consider making a video on how the casual/hobby grower might be able to cut down on the use of plastics while still advancing in the hobby? Thanks!
As far as petri dishes go you can purchase autoclavable plastic dishes which can be used many times over. Instead of polystyrene go for polypropylene or polycarbonate, these handle much higher heats as the cheap disposables melt in the autoclave. You can also try borosilicate glass dishes, but then you have to worry about them breaking, and they don't often fit together as nicely. Instead of plastic mushroom grow bags you could always go with the bottle method and reuse large glass mason jars. Hope this helps!
its not very often I'm impressed but this video really caused me to say wow I need to ask - have you checked out the website called gregs mushroom grower look for it on google
I wish I can post my lab video, I followed all your steps and list and build my lap. thank you so much for all the info... everyone complements it. I build the flow hood hepa as well from your technical drawing and works like a charm. no one thought as am female I can accomplish this but I guess I use my computer engineering skills to get this done!
@@tech1media842 Im saying that you could upload your videos (to your channel or somewhere else) and show what you're doing, its the best part of the mushroom community, it doesn't matter if some people somehow believed that you couldn't. you already proved them wrong, the videos are just the nails to their coffins. I'm Starting a urban farm from 0, so far I just got tons of written research some lions mane and oyster liquid culture on the fridge, and im waiting for my all american to arrive, ill start expanding mycelium in liquid culture and then I hope to upload some content about the workflow in the farm and that kind of things, just another drop in the bucket of knowledge
I have a gota bot of a hack for that home grower having trouble battling contams in the still air box tek realm. Get a 4'x6' table and a kiddie tent. Top it, insert still air box, Lysol that like it holds the plague and zip your torso up in the tent as it sets on the table. Makes double secure. Its no airflow hood but its only 20 bucks for about 90% better conditions in a micro enviornment that is highly mobile.
Open air will work If your clean and in a area that has still air around you. Only need an expensive hood if your going to make a living out of it. You must have a Pressure cooker!
It's rare that i am surprized but this video certainly made me say wow I need to ask - have you looked at the resource called gregs mushroom grower just google for it
I have just common presure coker without manometer ... is ok ? If yes how much time should be used to sterilize my agar mixture and grains for micelium incoculation?
Hey Tony, thanks for the awesome videos. I need your help. In the UK we cannot easily get a pressure canner to pasteurise grain for making grain spawn. Any alternative ways to make pasteurise grain in bottles without using a pressure canner? Cheers James
Pressurizing just decreases the time requirement - no normal bacteria can survive prolonged boiling. If you use a large pot with a rack in the bottom to lift your jars/bottles off of the surface and boil continuously for 90 minutes you will sterilize the contents. Here is a link to a reputable source (a university-based center focused on home food preservation) that discusses sterilizing empty jars or jelly/jam filled jars with boiling water alone in 10 minutes. Grain is not going to transfer heat nearly that well and is both dirtier and a friendlier environment than high sugar/high acid jam, so it needs longer to be sure. nchfp.uga.edu/publications/nchfp/factsheets/sterilizing.html
@@joatmon8954 anything that could ever be remotely considered unsafe is illegal there. Pocket knives, dado stacks on table saws, fireworks, carrying tools in your vehicle without a licence, etc.
Hey i was just wondering how do i make my lab postive pressure in a 3 metre by 3 metre green house ? If its outside will there be more airborn contams if i was to have a fan blowing in fresh air whith vent for exhaust please somebody reply
I’m planning to build a lab within the next month or so that i hope to one day turn into a business. I have money set aside but not enough to get the best of everything all at once. I’m wondering if you’d recommend saving by getting a standard pressure cooker to start and then the electric all American later, or getting a smaller bonsai hood to start and upsizing later. I don’t have tools or DIY skill so building one is out. Whichever will reduce pain points in the fledgling lab will be what I go with. Any insight or advice will be appreciated!
the all american vs a presto is no difference other than brand. both will fit 9 jars both do the same job. yes one is cleaner more rigid and the perk of electric may be enticing to some for the all american.. but the presto will do you just fine. you can start with a SAB and it works fine but it is hard to keep up when having alot of production G2G is a pain in a SAB and grain to bulk is a pain as well but it does get the job done. your looking to about 1000$ for a complete flow hood brand new. if your not able to DIY your best bet is to look for auctions/lab liquidators and even schools. i would recommend starting with a 23qt presto and a SAB and see how much you like the hobby you will get contamination at first but once you hone in your skills it will work just as good as a flow hood. it took me personally a few months to not get contamination in any of my transfers. PS dont fall for the short cuts there is alot of misinformation in this hobby use a SAB or a real laminar flow hood don't waste your time with speculated theory like "box fan flow hoods" understand the principles of why the SAB and laminar flow hood work and you will be able to debunk the BS cheap corner cutters that waste money
Does anyone know where you get that lifted metal tray stand that sits in front of the laminar flow hood? I'm going to build a flow hood and would love to have something that elevates my material to ensure it has the proper clean air flow around it.
They will leak a little bit, you can always replaced the seal, but eventually they will get deformed a little bit. the first two I had to replace got warped because they ran out of water- that was my fault haha
Tony I put 8 cakes in an expensive fruiting chamber I bought recently. The humidity remains between 90 and 99 and the temperature remains at about 73 degrees. The lights are on twelve hours a day and they sit on the top rack of my fruiting chamber with an ionizer mist above them. On the floor there is a heater with lava rock above it and water to add humidity. I put these cakes in 15 days ago and although they have pinned and are free of contams they have not sent out any fruit. I can't post photos here but they look good. Is there anything I can do besides wait? Thanks for any suggestions you may be able to offer. Much appreciated. Love all your videos.
I do not, the filter was from AAF Flanders, they do the sell them online. The sterilizer came from Balkoswitch enterprises... Not sure if you can get them on Amazon or not.
Cheers for this, I been tryin to find out about "pics mushroom cultivation" for a while now, and I think this has helped. Ever heard of - Heenyan Mushroom Organifiction - (just google it ) ? Ive heard some unbelievable things about it and my friend got cool results with it.
@@jesusrojassi I personally have found that reading books pigeon holds your capabilities into only doing it one way which is not quite a good thing to do if you experiment try things outside of the box you'll find that a lot of the mycology books out there scare you into believing that if you don't do everything 100% sterile you will not be successful I can tell you 100% fact that that is not true
@@jesusrojassi did you study a particular type or were you just studying broadly and did you do it on your own or was it done through University the only reason I'm asking is I'm curious
Is that the only pressure cooker you use at a time? The 6 5lb electric one? I thought you were using some kind of larger drum sterilizer for like 30 to 40 bags at a time ..if so how many bags do you typically make a day? As far as sawdust bags . love your videos man:)
Yup! Just that sterilizer- we aren't growing fresh mushrooms commercially right now, just doing it for fun/content/experimentation ect. we work with other amazing growers for the mushrooms in our extracts.
@@FreshCapMushrooms oh ok.i thought you were growing more ..not necessarily commercial but like enough to sell at farmers markets n have money on the side. You should definitely make a vid on cheap steamed drum sterilizers. I find that bubbas barresl are too expensive although they are awesome.but out of many peoples price range ..there is cheaper ways I found they do in india and Africa.its awesome .
Use 70%. There are many studies from WHO and others showing 70% is better at disinfecting because the extra water in the solution is able to penetrate the cell walls of the bacteria allowing the alcohol a way into the cell. 90% can't get in the cell as well as the diluted 70%. Of course if you can only get 90, use that or dilute it yourself.
Thank you Tony for your Professional way about explaining and setting a Example for The people such as myself! When I am trying to find a way to Feed myself, Lord Willing couple children and Turn that ever present PROFILE of a lost really lost Soul! Only Looked at as a SHADOW of ways,gone for Ten Years Truth be Told..... Are the profit margins there not to be rich by no means But be the Honest Productive Member of the Community ,My area South Eastern United States of America the small Growers are at Farmers Markets once a week, But I got to work like the Amazing Mycelium and let it clean My Name Never killed any one. But not be thought of as that Beloved and Super Talented Sub Culture that Thanks to them and For Utub Gave me the Blessed things to Better myself and turn this spot seen by every one into something I'm self and Family can be Proud off! Its just that time to forget the Bros , And Be the Man in my area , Don't Forget the Mark of The Green Star That's been A Logo I've Always Wanted To let it do what it Wii do Leave the Mark Of the Man , We all got to get a plan and Try! That Stare In my Dream It Will Not let Me Down Thank you Ser!!
@@shreygupta4113 Might be a strain issue. I literally just cut a slice across the bag and it friuts right across. I might have a video on the process soon!
If you can, get parafilm. It's easier to work with, adheres better to moist surfaces, doesn't leave a sticky mess when you take it off, and won't get soaked and wick possibly contaminated moisture into the dish. But if you can't get any easily, or you're on a very tight budget - yes, micropore tape can absolutely work.
@@93FilmsandMedia got very inconsistent results with pressure cooker. agar in petri dishes sterelised in pressure cooker did much worse than sterelised in bottle and then poured into dishes. also always searching for alternative ways to do things.
hello I'm from Kazakhstan, last year I made your video "cold-water pasteurization". Thank you very much !!! Will you show me the way out of the fog? I'm sorry to use my slippers, help me make a request (I do not know what to do)
I’m having some serious trouble with my spawn at the moment, every single time I inoculate, a couple days later I’m getting cobweb mold, i did everything right with the teks I’ve followed, could my setting that I put the jars to colonize have stagnant air or something? Any help is appreciated greatly
Why can't you reuse petri dishes? Isn't it possible to just clean, sterilize & reuse... ...wait, let me guess, to sterilize means to pressure cook for which plastic doesn't has the necessary heat resistance
